Baicalin induces apoptosis in SW480 cells through downregulation of the SP1 transcription factor

Colorectal cancer occurs throughout the world but is most common in developed countries. Cancer progression is believed to be driven by genetic mutations in this complex condition. Risk factors for developing colorectal cancer include a genetic family history, long-term ulcerative colitis, and colon...

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Autores principales: Ma, Wenkang, Liu, Xueyuan, Du, Wei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Lippincott Williams & Wilkins 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6365257/
https://www.ncbi.nlm.nih.gov/pubmed/30362980
http://dx.doi.org/10.1097/CAD.0000000000000708
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author Ma, Wenkang
Liu, Xueyuan
Du, Wei
author_facet Ma, Wenkang
Liu, Xueyuan
Du, Wei
author_sort Ma, Wenkang
collection PubMed
description Colorectal cancer occurs throughout the world but is most common in developed countries. Cancer progression is believed to be driven by genetic mutations in this complex condition. Risk factors for developing colorectal cancer include a genetic family history, long-term ulcerative colitis, and colonic polyps. The use of baicalin has been reported to be clinically efficacious against colon tumors in Asian countries despite an unclear mechanism of action. Several cancers have been found to be biologically dependent on the specificity protein 1 (sp1) transcription factor family. We hypothesized that baicalin may exert its chemotherapeutic effects by sp1 downregulation. Using the SW480 human colorectal cancer cell line, we investigated the physiological properties of baicalin. Our experiments were designed toward clarifying three goals: (a) to determine the mRNA expression profile of transcription factors in colorectal cancer patients using a microarray-based analysis; (b) to determine the effects of baicalin on the sp1 transcription factor with western blotting and reporter cell assays; and (c) to contrast the effects of mithramycin-A (an sp1 transcription factor inhibitor) and baicalin using western blotting and reporter cell assays. Both baicalin and mithramycin-A downregulated sp1 expression, attenuated SW480 cell proliferation, and increased cell apoptosis. Baicalin inhibited sp1 expression and led to SW480 apoptosis, thus clarifying the effect of this traditional Chinese medicine compound in the treatment of colon cancer.
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spelling pubmed-63652572019-02-20 Baicalin induces apoptosis in SW480 cells through downregulation of the SP1 transcription factor Ma, Wenkang Liu, Xueyuan Du, Wei Anticancer Drugs Preclinical Reports Colorectal cancer occurs throughout the world but is most common in developed countries. Cancer progression is believed to be driven by genetic mutations in this complex condition. Risk factors for developing colorectal cancer include a genetic family history, long-term ulcerative colitis, and colonic polyps. The use of baicalin has been reported to be clinically efficacious against colon tumors in Asian countries despite an unclear mechanism of action. Several cancers have been found to be biologically dependent on the specificity protein 1 (sp1) transcription factor family. We hypothesized that baicalin may exert its chemotherapeutic effects by sp1 downregulation. Using the SW480 human colorectal cancer cell line, we investigated the physiological properties of baicalin. Our experiments were designed toward clarifying three goals: (a) to determine the mRNA expression profile of transcription factors in colorectal cancer patients using a microarray-based analysis; (b) to determine the effects of baicalin on the sp1 transcription factor with western blotting and reporter cell assays; and (c) to contrast the effects of mithramycin-A (an sp1 transcription factor inhibitor) and baicalin using western blotting and reporter cell assays. Both baicalin and mithramycin-A downregulated sp1 expression, attenuated SW480 cell proliferation, and increased cell apoptosis. Baicalin inhibited sp1 expression and led to SW480 apoptosis, thus clarifying the effect of this traditional Chinese medicine compound in the treatment of colon cancer. Lippincott Williams & Wilkins 2019-02 2019-01-11 /pmc/articles/PMC6365257/ /pubmed/30362980 http://dx.doi.org/10.1097/CAD.0000000000000708 Text en Copyright © 2018 The Author(s). Published by Wolters Kluwer Health, Inc. This is an open-access article distributed under the terms of the Creative Commons Attribution-Non Commercial-No Derivatives License 4.0 (http://creativecommons.org/licenses/by-nc-nd/4.0/) (CCBY-NC-ND), where it is permissible to download and share the work provided it is properly cited. The work cannot be changed in any way or used commercially without permission from the journal. http://creativecommons.org/licenses/by-nc-nd/4.0/
spellingShingle Preclinical Reports
Ma, Wenkang
Liu, Xueyuan
Du, Wei
Baicalin induces apoptosis in SW480 cells through downregulation of the SP1 transcription factor
title Baicalin induces apoptosis in SW480 cells through downregulation of the SP1 transcription factor
title_full Baicalin induces apoptosis in SW480 cells through downregulation of the SP1 transcription factor
title_fullStr Baicalin induces apoptosis in SW480 cells through downregulation of the SP1 transcription factor
title_full_unstemmed Baicalin induces apoptosis in SW480 cells through downregulation of the SP1 transcription factor
title_short Baicalin induces apoptosis in SW480 cells through downregulation of the SP1 transcription factor
title_sort baicalin induces apoptosis in sw480 cells through downregulation of the sp1 transcription factor
topic Preclinical Reports
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6365257/
https://www.ncbi.nlm.nih.gov/pubmed/30362980
http://dx.doi.org/10.1097/CAD.0000000000000708
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