Effect of low storage temperature on pollen viability of fifteen herbaceous peonies

In order to develop rich new varieties of peony flowers and solve the problems associated with non-synchronised flowering, a study was conducted to investigate pollen viability of fifteen herbaceous peony cultivars. Pollen was stored for more than one year under four storage conditions (4 °C, −4 °C,...

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Autores principales: Du, Guangcong, Xu, Jinguang, Gao, Chengrong, Lu, Jie, Li, Qian, Du, Jie, Lv, Mengwen, Sun, Xia
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6365399/
https://www.ncbi.nlm.nih.gov/pubmed/30775270
http://dx.doi.org/10.1016/j.btre.2019.e00309
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author Du, Guangcong
Xu, Jinguang
Gao, Chengrong
Lu, Jie
Li, Qian
Du, Jie
Lv, Mengwen
Sun, Xia
author_facet Du, Guangcong
Xu, Jinguang
Gao, Chengrong
Lu, Jie
Li, Qian
Du, Jie
Lv, Mengwen
Sun, Xia
author_sort Du, Guangcong
collection PubMed
description In order to develop rich new varieties of peony flowers and solve the problems associated with non-synchronised flowering, a study was conducted to investigate pollen viability of fifteen herbaceous peony cultivars. Pollen was stored for more than one year under four storage conditions (4 °C, −4 °C, −20 °C, and −76 °C). In vitro germination and the I(2)-KI staining method were used to test pollen viability. For all cultivars, pollen stored at −76 °C showed a significantly slower rate of viability reduction when compared to all the other storage conditions. The differential results obtained using different pollen viability assays confirmed that the in vitro germination test showed lower viability compared to I(2)-KI staining method. The pollen tube length grew longer as storage temperature rose such as ‘Hongfeng’. From the present study, it has been proved that the I(2)-KI staining method is more convenient and reliable than the in vitro germination test. Herbaceous peony pollen can be stored at 4 °C for hand-pollination among cultivars having non-synchronised flowering in a season, and that different storage temperature is suited to different herbaceous peony pollen for more than one year.
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spelling pubmed-63653992019-02-15 Effect of low storage temperature on pollen viability of fifteen herbaceous peonies Du, Guangcong Xu, Jinguang Gao, Chengrong Lu, Jie Li, Qian Du, Jie Lv, Mengwen Sun, Xia Biotechnol Rep (Amst) Article In order to develop rich new varieties of peony flowers and solve the problems associated with non-synchronised flowering, a study was conducted to investigate pollen viability of fifteen herbaceous peony cultivars. Pollen was stored for more than one year under four storage conditions (4 °C, −4 °C, −20 °C, and −76 °C). In vitro germination and the I(2)-KI staining method were used to test pollen viability. For all cultivars, pollen stored at −76 °C showed a significantly slower rate of viability reduction when compared to all the other storage conditions. The differential results obtained using different pollen viability assays confirmed that the in vitro germination test showed lower viability compared to I(2)-KI staining method. The pollen tube length grew longer as storage temperature rose such as ‘Hongfeng’. From the present study, it has been proved that the I(2)-KI staining method is more convenient and reliable than the in vitro germination test. Herbaceous peony pollen can be stored at 4 °C for hand-pollination among cultivars having non-synchronised flowering in a season, and that different storage temperature is suited to different herbaceous peony pollen for more than one year. Elsevier 2019-01-17 /pmc/articles/PMC6365399/ /pubmed/30775270 http://dx.doi.org/10.1016/j.btre.2019.e00309 Text en © 2019 The Authors http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Article
Du, Guangcong
Xu, Jinguang
Gao, Chengrong
Lu, Jie
Li, Qian
Du, Jie
Lv, Mengwen
Sun, Xia
Effect of low storage temperature on pollen viability of fifteen herbaceous peonies
title Effect of low storage temperature on pollen viability of fifteen herbaceous peonies
title_full Effect of low storage temperature on pollen viability of fifteen herbaceous peonies
title_fullStr Effect of low storage temperature on pollen viability of fifteen herbaceous peonies
title_full_unstemmed Effect of low storage temperature on pollen viability of fifteen herbaceous peonies
title_short Effect of low storage temperature on pollen viability of fifteen herbaceous peonies
title_sort effect of low storage temperature on pollen viability of fifteen herbaceous peonies
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6365399/
https://www.ncbi.nlm.nih.gov/pubmed/30775270
http://dx.doi.org/10.1016/j.btre.2019.e00309
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