Effect of low storage temperature on pollen viability of fifteen herbaceous peonies
In order to develop rich new varieties of peony flowers and solve the problems associated with non-synchronised flowering, a study was conducted to investigate pollen viability of fifteen herbaceous peony cultivars. Pollen was stored for more than one year under four storage conditions (4 °C, −4 °C,...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6365399/ https://www.ncbi.nlm.nih.gov/pubmed/30775270 http://dx.doi.org/10.1016/j.btre.2019.e00309 |
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author | Du, Guangcong Xu, Jinguang Gao, Chengrong Lu, Jie Li, Qian Du, Jie Lv, Mengwen Sun, Xia |
author_facet | Du, Guangcong Xu, Jinguang Gao, Chengrong Lu, Jie Li, Qian Du, Jie Lv, Mengwen Sun, Xia |
author_sort | Du, Guangcong |
collection | PubMed |
description | In order to develop rich new varieties of peony flowers and solve the problems associated with non-synchronised flowering, a study was conducted to investigate pollen viability of fifteen herbaceous peony cultivars. Pollen was stored for more than one year under four storage conditions (4 °C, −4 °C, −20 °C, and −76 °C). In vitro germination and the I(2)-KI staining method were used to test pollen viability. For all cultivars, pollen stored at −76 °C showed a significantly slower rate of viability reduction when compared to all the other storage conditions. The differential results obtained using different pollen viability assays confirmed that the in vitro germination test showed lower viability compared to I(2)-KI staining method. The pollen tube length grew longer as storage temperature rose such as ‘Hongfeng’. From the present study, it has been proved that the I(2)-KI staining method is more convenient and reliable than the in vitro germination test. Herbaceous peony pollen can be stored at 4 °C for hand-pollination among cultivars having non-synchronised flowering in a season, and that different storage temperature is suited to different herbaceous peony pollen for more than one year. |
format | Online Article Text |
id | pubmed-6365399 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-63653992019-02-15 Effect of low storage temperature on pollen viability of fifteen herbaceous peonies Du, Guangcong Xu, Jinguang Gao, Chengrong Lu, Jie Li, Qian Du, Jie Lv, Mengwen Sun, Xia Biotechnol Rep (Amst) Article In order to develop rich new varieties of peony flowers and solve the problems associated with non-synchronised flowering, a study was conducted to investigate pollen viability of fifteen herbaceous peony cultivars. Pollen was stored for more than one year under four storage conditions (4 °C, −4 °C, −20 °C, and −76 °C). In vitro germination and the I(2)-KI staining method were used to test pollen viability. For all cultivars, pollen stored at −76 °C showed a significantly slower rate of viability reduction when compared to all the other storage conditions. The differential results obtained using different pollen viability assays confirmed that the in vitro germination test showed lower viability compared to I(2)-KI staining method. The pollen tube length grew longer as storage temperature rose such as ‘Hongfeng’. From the present study, it has been proved that the I(2)-KI staining method is more convenient and reliable than the in vitro germination test. Herbaceous peony pollen can be stored at 4 °C for hand-pollination among cultivars having non-synchronised flowering in a season, and that different storage temperature is suited to different herbaceous peony pollen for more than one year. Elsevier 2019-01-17 /pmc/articles/PMC6365399/ /pubmed/30775270 http://dx.doi.org/10.1016/j.btre.2019.e00309 Text en © 2019 The Authors http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Article Du, Guangcong Xu, Jinguang Gao, Chengrong Lu, Jie Li, Qian Du, Jie Lv, Mengwen Sun, Xia Effect of low storage temperature on pollen viability of fifteen herbaceous peonies |
title | Effect of low storage temperature on pollen viability of fifteen herbaceous peonies |
title_full | Effect of low storage temperature on pollen viability of fifteen herbaceous peonies |
title_fullStr | Effect of low storage temperature on pollen viability of fifteen herbaceous peonies |
title_full_unstemmed | Effect of low storage temperature on pollen viability of fifteen herbaceous peonies |
title_short | Effect of low storage temperature on pollen viability of fifteen herbaceous peonies |
title_sort | effect of low storage temperature on pollen viability of fifteen herbaceous peonies |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6365399/ https://www.ncbi.nlm.nih.gov/pubmed/30775270 http://dx.doi.org/10.1016/j.btre.2019.e00309 |
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