Combined quantification of intracellular (phospho-)proteins and transcriptomics from fixed single cells

Environmental stimuli often lead to heterogeneous cellular responses and transcriptional output. We developed single-cell RNA and Immunodetection (RAID) to allow combined analysis of the transcriptome and intracellular (phospho-)proteins from fixed single cells. RAID successfully recapitulated diffe...

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Detalles Bibliográficos
Autores principales: Gerlach, Jan. P., van Buggenum, Jessie A. G., Tanis, Sabine E. J., Hogeweg, Mark, Heuts, Branco M. H., Muraro, Mauro J., Elze, Lisa, Rivello, Francesca, Rakszewska, Agata, van Oudenaarden, Alexander, Huck, Wilhelm T. S., Stunnenberg, Hendrik G., Mulder, Klaas W.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2019
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6365588/
https://www.ncbi.nlm.nih.gov/pubmed/30728416
http://dx.doi.org/10.1038/s41598-018-37977-7
Descripción
Sumario:Environmental stimuli often lead to heterogeneous cellular responses and transcriptional output. We developed single-cell RNA and Immunodetection (RAID) to allow combined analysis of the transcriptome and intracellular (phospho-)proteins from fixed single cells. RAID successfully recapitulated differentiation-state changes at the protein and mRNA level in human keratinocytes. Furthermore, we show that differentiated keratinocytes that retain high phosphorylated FAK levels, a feature associated with stem cells, also express a selection of stem cell associated transcripts. Our data demonstrates that RAID allows investigation of heterogeneous cellular responses to environmental signals at the mRNA and phospho-proteome level.

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