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miR-29a ameliorates ischemic injury of astrocytes in vitro by targeting the water channel protein aquaporin 4
Ischemic stroke is the main cause of brain injury and results in a high rate of morbidity, disability and mortality. In the present study, we aimed to determine whether miR-29a played a protective role in oxygen glucose deprivation (OGD) injury via regulation of the water channel protein aquaporin 4...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
D.A. Spandidos
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6365700/ https://www.ncbi.nlm.nih.gov/pubmed/30628716 http://dx.doi.org/10.3892/or.2019.6961 |
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author | Zheng, Yueying Pan, Caifei Chen, Manli Pei, Aijie Xie, Liwei Zhu, Shengmei |
author_facet | Zheng, Yueying Pan, Caifei Chen, Manli Pei, Aijie Xie, Liwei Zhu, Shengmei |
author_sort | Zheng, Yueying |
collection | PubMed |
description | Ischemic stroke is the main cause of brain injury and results in a high rate of morbidity, disability and mortality. In the present study, we aimed to determine whether miR-29a played a protective role in oxygen glucose deprivation (OGD) injury via regulation of the water channel protein aquaporin 4 (AQP4). Real-time PCR and western blotting were used to assess miR-29a levels and AQP4 protein levels, respectively. Apoptosis was detected by flow cytometry, and lactate dehydrogenase (LDH) was determined by enzyme-linked immunosorbent assay (ELISA). Overexpression of miR-29a was significantly downregulated in OGD-induced primary astrocytes, and transfection with a miR-29a mimic decreased LDH release and apoptosis, and improved cell health in OGD-induced astrocytes. AQP4 was the target of miR-29a, which suppressed AQP4 expression, and knockdown of AQP4 mitigated OGD-induced astrocyte injury. Furthermore, miR-29a regulated AQP4 expression in OGD-induced astrocytes. AQP4 exacerbated astrocyte injury following ischemic stroke, and knockdown of AQP4 protected OGD/RX-induced primary cultured astrocytes against injury. The effect of miR-29a inhibitor on primary astrocytes was lost following AQP4 knockdown. These findings indicated that miR-29a prevented astrocyte injury in vitro by inhibiting AQP4. Thus, miR-29a may protect primary cultured astrocytes after OGD-induced injury by targeting AQP4, and may be a potential therapeutic target for ischemic injury of astrocytes. |
format | Online Article Text |
id | pubmed-6365700 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | D.A. Spandidos |
record_format | MEDLINE/PubMed |
spelling | pubmed-63657002019-02-23 miR-29a ameliorates ischemic injury of astrocytes in vitro by targeting the water channel protein aquaporin 4 Zheng, Yueying Pan, Caifei Chen, Manli Pei, Aijie Xie, Liwei Zhu, Shengmei Oncol Rep Articles Ischemic stroke is the main cause of brain injury and results in a high rate of morbidity, disability and mortality. In the present study, we aimed to determine whether miR-29a played a protective role in oxygen glucose deprivation (OGD) injury via regulation of the water channel protein aquaporin 4 (AQP4). Real-time PCR and western blotting were used to assess miR-29a levels and AQP4 protein levels, respectively. Apoptosis was detected by flow cytometry, and lactate dehydrogenase (LDH) was determined by enzyme-linked immunosorbent assay (ELISA). Overexpression of miR-29a was significantly downregulated in OGD-induced primary astrocytes, and transfection with a miR-29a mimic decreased LDH release and apoptosis, and improved cell health in OGD-induced astrocytes. AQP4 was the target of miR-29a, which suppressed AQP4 expression, and knockdown of AQP4 mitigated OGD-induced astrocyte injury. Furthermore, miR-29a regulated AQP4 expression in OGD-induced astrocytes. AQP4 exacerbated astrocyte injury following ischemic stroke, and knockdown of AQP4 protected OGD/RX-induced primary cultured astrocytes against injury. The effect of miR-29a inhibitor on primary astrocytes was lost following AQP4 knockdown. These findings indicated that miR-29a prevented astrocyte injury in vitro by inhibiting AQP4. Thus, miR-29a may protect primary cultured astrocytes after OGD-induced injury by targeting AQP4, and may be a potential therapeutic target for ischemic injury of astrocytes. D.A. Spandidos 2019-03 2019-01-09 /pmc/articles/PMC6365700/ /pubmed/30628716 http://dx.doi.org/10.3892/or.2019.6961 Text en Copyright: © Zheng et al. This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made. |
spellingShingle | Articles Zheng, Yueying Pan, Caifei Chen, Manli Pei, Aijie Xie, Liwei Zhu, Shengmei miR-29a ameliorates ischemic injury of astrocytes in vitro by targeting the water channel protein aquaporin 4 |
title | miR-29a ameliorates ischemic injury of astrocytes in vitro by targeting the water channel protein aquaporin 4 |
title_full | miR-29a ameliorates ischemic injury of astrocytes in vitro by targeting the water channel protein aquaporin 4 |
title_fullStr | miR-29a ameliorates ischemic injury of astrocytes in vitro by targeting the water channel protein aquaporin 4 |
title_full_unstemmed | miR-29a ameliorates ischemic injury of astrocytes in vitro by targeting the water channel protein aquaporin 4 |
title_short | miR-29a ameliorates ischemic injury of astrocytes in vitro by targeting the water channel protein aquaporin 4 |
title_sort | mir-29a ameliorates ischemic injury of astrocytes in vitro by targeting the water channel protein aquaporin 4 |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6365700/ https://www.ncbi.nlm.nih.gov/pubmed/30628716 http://dx.doi.org/10.3892/or.2019.6961 |
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