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MicroRNA-3653 inhibits the growth and metastasis of hepatocellular carcinoma by inhibiting ITGB1
microRNAs (miRNAs) play critical roles in hepatocellular carcinoma (HCC). However, the expression and biological function of miR-3653 in HCC remain unknown. The present study demonstrated that miR-3653 expression was significantly decreased in HCC tissues and cells using qRT-PCR. A decreased miR-365...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
D.A. Spandidos
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6365710/ https://www.ncbi.nlm.nih.gov/pubmed/30664185 http://dx.doi.org/10.3892/or.2019.6971 |
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author | Zhang, Lijuan Zhang, Tao Deng, Zerun Sun, Lihua |
author_facet | Zhang, Lijuan Zhang, Tao Deng, Zerun Sun, Lihua |
author_sort | Zhang, Lijuan |
collection | PubMed |
description | microRNAs (miRNAs) play critical roles in hepatocellular carcinoma (HCC). However, the expression and biological function of miR-3653 in HCC remain unknown. The present study demonstrated that miR-3653 expression was significantly decreased in HCC tissues and cells using qRT-PCR. A decreased miR-3653 level was associated with unfavorable clinical features and poor prognosis of HCC patients. MTT, BrdU, Transwell and western blot assays showed that miR-3653 overexpression inhibited the growth, migration, invasion and epithelial-mesenchymal transition (EMT) of HCCLM3 cells while its knockdown promoted the growth and metastatic ability of Hep3B cells. In vivo experiments showed that miR-3653 overexpression inhibited the subcutaneous and the lung metastasis of HCCLM3 cells in nude mice. Mechanistically, integrin-β1 (ITGB1) was identified to be the downstream target of miR-3653 in HCC. ITGB1 overexpression reversed the inhibitory effects of miR-3653 on the growth, metastasis and EMT of HCCLM3 cells. |
format | Online Article Text |
id | pubmed-6365710 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | D.A. Spandidos |
record_format | MEDLINE/PubMed |
spelling | pubmed-63657102019-02-23 MicroRNA-3653 inhibits the growth and metastasis of hepatocellular carcinoma by inhibiting ITGB1 Zhang, Lijuan Zhang, Tao Deng, Zerun Sun, Lihua Oncol Rep Articles microRNAs (miRNAs) play critical roles in hepatocellular carcinoma (HCC). However, the expression and biological function of miR-3653 in HCC remain unknown. The present study demonstrated that miR-3653 expression was significantly decreased in HCC tissues and cells using qRT-PCR. A decreased miR-3653 level was associated with unfavorable clinical features and poor prognosis of HCC patients. MTT, BrdU, Transwell and western blot assays showed that miR-3653 overexpression inhibited the growth, migration, invasion and epithelial-mesenchymal transition (EMT) of HCCLM3 cells while its knockdown promoted the growth and metastatic ability of Hep3B cells. In vivo experiments showed that miR-3653 overexpression inhibited the subcutaneous and the lung metastasis of HCCLM3 cells in nude mice. Mechanistically, integrin-β1 (ITGB1) was identified to be the downstream target of miR-3653 in HCC. ITGB1 overexpression reversed the inhibitory effects of miR-3653 on the growth, metastasis and EMT of HCCLM3 cells. D.A. Spandidos 2019-03 2019-01-16 /pmc/articles/PMC6365710/ /pubmed/30664185 http://dx.doi.org/10.3892/or.2019.6971 Text en Copyright: © Zhang et al. This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made. |
spellingShingle | Articles Zhang, Lijuan Zhang, Tao Deng, Zerun Sun, Lihua MicroRNA-3653 inhibits the growth and metastasis of hepatocellular carcinoma by inhibiting ITGB1 |
title | MicroRNA-3653 inhibits the growth and metastasis of hepatocellular carcinoma by inhibiting ITGB1 |
title_full | MicroRNA-3653 inhibits the growth and metastasis of hepatocellular carcinoma by inhibiting ITGB1 |
title_fullStr | MicroRNA-3653 inhibits the growth and metastasis of hepatocellular carcinoma by inhibiting ITGB1 |
title_full_unstemmed | MicroRNA-3653 inhibits the growth and metastasis of hepatocellular carcinoma by inhibiting ITGB1 |
title_short | MicroRNA-3653 inhibits the growth and metastasis of hepatocellular carcinoma by inhibiting ITGB1 |
title_sort | microrna-3653 inhibits the growth and metastasis of hepatocellular carcinoma by inhibiting itgb1 |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6365710/ https://www.ncbi.nlm.nih.gov/pubmed/30664185 http://dx.doi.org/10.3892/or.2019.6971 |
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