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Determination of donepezil in spiked rabbit plasma by high-performance liquid chromatography with fluorescence detection
The aim of this paper is to develop sensitive, accurate, reproducible and robust RP-HPLC with fluorescence detection for estimation of donepezil (DZ) in rabbit plasma using silodosin as the internal standard (IS). The prepared samples were quantified on reversed phase column Luna C(18(2)) (150 × 4.6...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Royal Society
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6366186/ https://www.ncbi.nlm.nih.gov/pubmed/30800388 http://dx.doi.org/10.1098/rsos.181476 |
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author | Mohamed, Fardous A. Khashaba, Pakinaz Y. Shahin, Reem Y. El-Wekil, Mohamed M. |
author_facet | Mohamed, Fardous A. Khashaba, Pakinaz Y. Shahin, Reem Y. El-Wekil, Mohamed M. |
author_sort | Mohamed, Fardous A. |
collection | PubMed |
description | The aim of this paper is to develop sensitive, accurate, reproducible and robust RP-HPLC with fluorescence detection for estimation of donepezil (DZ) in rabbit plasma using silodosin as the internal standard (IS). The prepared samples were quantified on reversed phase column Luna C(18(2)) (150 × 4.6 mm i.d., 5 µm particle size) operated at room temperature using the mobile phase consisting of methanol: 0.1% acetic acid (50 : 50, v/v) at a flow rate of 1 ml min(−1). The method was fully validated according to bioanalytical validation guidelines of FDA in terms of system suitability, selectivity, sensitivity, precision and stability. It was found that the increase in peak areas followed the increase of DZ concentration in the range of 2.56–200.00 ng ml(−1) with LOD of 0.85 ng ml(−1). The method was successfully applied for the determination of DZ in rabbit plasma using manual shaking dispersive liquid–liquid microextraction. |
format | Online Article Text |
id | pubmed-6366186 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | The Royal Society |
record_format | MEDLINE/PubMed |
spelling | pubmed-63661862019-02-22 Determination of donepezil in spiked rabbit plasma by high-performance liquid chromatography with fluorescence detection Mohamed, Fardous A. Khashaba, Pakinaz Y. Shahin, Reem Y. El-Wekil, Mohamed M. R Soc Open Sci Chemistry The aim of this paper is to develop sensitive, accurate, reproducible and robust RP-HPLC with fluorescence detection for estimation of donepezil (DZ) in rabbit plasma using silodosin as the internal standard (IS). The prepared samples were quantified on reversed phase column Luna C(18(2)) (150 × 4.6 mm i.d., 5 µm particle size) operated at room temperature using the mobile phase consisting of methanol: 0.1% acetic acid (50 : 50, v/v) at a flow rate of 1 ml min(−1). The method was fully validated according to bioanalytical validation guidelines of FDA in terms of system suitability, selectivity, sensitivity, precision and stability. It was found that the increase in peak areas followed the increase of DZ concentration in the range of 2.56–200.00 ng ml(−1) with LOD of 0.85 ng ml(−1). The method was successfully applied for the determination of DZ in rabbit plasma using manual shaking dispersive liquid–liquid microextraction. The Royal Society 2019-01-16 /pmc/articles/PMC6366186/ /pubmed/30800388 http://dx.doi.org/10.1098/rsos.181476 Text en © 2019 The Authors. http://creativecommons.org/licenses/by/4.0/ Published by the Royal Society under the terms of the Creative Commons Attribution License http://creativecommons.org/licenses/by/4.0/, which permits unrestricted use, provided the original author and source are credited. |
spellingShingle | Chemistry Mohamed, Fardous A. Khashaba, Pakinaz Y. Shahin, Reem Y. El-Wekil, Mohamed M. Determination of donepezil in spiked rabbit plasma by high-performance liquid chromatography with fluorescence detection |
title | Determination of donepezil in spiked rabbit plasma by high-performance liquid chromatography with fluorescence detection |
title_full | Determination of donepezil in spiked rabbit plasma by high-performance liquid chromatography with fluorescence detection |
title_fullStr | Determination of donepezil in spiked rabbit plasma by high-performance liquid chromatography with fluorescence detection |
title_full_unstemmed | Determination of donepezil in spiked rabbit plasma by high-performance liquid chromatography with fluorescence detection |
title_short | Determination of donepezil in spiked rabbit plasma by high-performance liquid chromatography with fluorescence detection |
title_sort | determination of donepezil in spiked rabbit plasma by high-performance liquid chromatography with fluorescence detection |
topic | Chemistry |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6366186/ https://www.ncbi.nlm.nih.gov/pubmed/30800388 http://dx.doi.org/10.1098/rsos.181476 |
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