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Effects of Lipopolysaccharide on Human First Trimester Villous Cytotrophoblast Cell Function In Vitro(1)

It has been shown that adverse obstetrical outcomes such as pre-eclampsia and intrauterine growth retardation correlate with maternal infection. In this study, we investigated mechanisms involved in infection-associated abnormalities in cytotrophoblast function. Primary human first trimester cytotro...

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Autores principales: Li, Liping, Tu, Jiaoqin, Jiang, Yao, Zhou, Jie, Yabe, Shinichiro, Schust, Danny J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6366481/
https://www.ncbi.nlm.nih.gov/pubmed/26700943
http://dx.doi.org/10.1095/biolreprod.115.134627
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author Li, Liping
Tu, Jiaoqin
Jiang, Yao
Zhou, Jie
Yabe, Shinichiro
Schust, Danny J.
author_facet Li, Liping
Tu, Jiaoqin
Jiang, Yao
Zhou, Jie
Yabe, Shinichiro
Schust, Danny J.
author_sort Li, Liping
collection PubMed
description It has been shown that adverse obstetrical outcomes such as pre-eclampsia and intrauterine growth retardation correlate with maternal infection. In this study, we investigated mechanisms involved in infection-associated abnormalities in cytotrophoblast function. Primary human first trimester cytotrophoblast cells were isolated and treated with lipopolysaccharide (LPS). Levels of the cytokines and chemokines were measured and cytotrophoblast invasion was investigated. In addition, first trimester decidual macrophages were isolated and treated with the conditioned medium from LPS-treated cytotrophoblast cells, and macrophage migration was assessed. Coculturing decidual macrophages with cytotrophoblast cells was conducted to investigate macrophage costimulatory molecule and receptor expression and intracellular cytokine production. We found that LPS exposure increased cytotrophoblast production of pro-inflammatory cytokines tumor necrosis factor (TNF)-alpha, interleukin (IL)-1beta and IL-6, and chemokines IL-8, macrophage inflammatory protein (MIP)-1alpha, and CXCL12 in a dose-dependent manner. In addition, LPS decreased cytotrophoblast invasion, and its effect was Toll-like receptor 4 (TLR4)-dependent and partly TNF-alpha-dependent. Conditioned medium from LPS-stimulated cytotrophoblast cells increased decidual macrophage migration and this effect was partly TLR4-dependent. Furthermore, coculturing decidual macrophages with LPS-exposed cytotrophoblast cells up-regulated macrophage CD80 and CD86 expression and intracellular TNF-alpha and IL-12p40 production, while down-regulating macrophage CD206 and CD209 expression and intracellular IL-10 secretion. LPS-stimulated macrophages also inhibited cytotrophoblast invasion. In conclusion, our results indicate that LPS increases the production of a subset of proinflammatory cytokines and chemokines by human first trimester cytotrophoblast cells, decreases cytotrophoblast invasion, and alters the cross talk between cytotrophoblast cells and decidual macrophages.
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spelling pubmed-63664812019-02-15 Effects of Lipopolysaccharide on Human First Trimester Villous Cytotrophoblast Cell Function In Vitro(1) Li, Liping Tu, Jiaoqin Jiang, Yao Zhou, Jie Yabe, Shinichiro Schust, Danny J. Biol Reprod Pregnancy It has been shown that adverse obstetrical outcomes such as pre-eclampsia and intrauterine growth retardation correlate with maternal infection. In this study, we investigated mechanisms involved in infection-associated abnormalities in cytotrophoblast function. Primary human first trimester cytotrophoblast cells were isolated and treated with lipopolysaccharide (LPS). Levels of the cytokines and chemokines were measured and cytotrophoblast invasion was investigated. In addition, first trimester decidual macrophages were isolated and treated with the conditioned medium from LPS-treated cytotrophoblast cells, and macrophage migration was assessed. Coculturing decidual macrophages with cytotrophoblast cells was conducted to investigate macrophage costimulatory molecule and receptor expression and intracellular cytokine production. We found that LPS exposure increased cytotrophoblast production of pro-inflammatory cytokines tumor necrosis factor (TNF)-alpha, interleukin (IL)-1beta and IL-6, and chemokines IL-8, macrophage inflammatory protein (MIP)-1alpha, and CXCL12 in a dose-dependent manner. In addition, LPS decreased cytotrophoblast invasion, and its effect was Toll-like receptor 4 (TLR4)-dependent and partly TNF-alpha-dependent. Conditioned medium from LPS-stimulated cytotrophoblast cells increased decidual macrophage migration and this effect was partly TLR4-dependent. Furthermore, coculturing decidual macrophages with LPS-exposed cytotrophoblast cells up-regulated macrophage CD80 and CD86 expression and intracellular TNF-alpha and IL-12p40 production, while down-regulating macrophage CD206 and CD209 expression and intracellular IL-10 secretion. LPS-stimulated macrophages also inhibited cytotrophoblast invasion. In conclusion, our results indicate that LPS increases the production of a subset of proinflammatory cytokines and chemokines by human first trimester cytotrophoblast cells, decreases cytotrophoblast invasion, and alters the cross talk between cytotrophoblast cells and decidual macrophages. Oxford University Press 2016-02-01 /pmc/articles/PMC6366481/ /pubmed/26700943 http://dx.doi.org/10.1095/biolreprod.115.134627 Text en © 2016 by the Society for the Study of Reproduction, Inc. This article is available under a Creative Commons License 4.0 (Attribution-Non-Commercial), as described at http://creativecommons.org/licenses/by-nc/4.0.
spellingShingle Pregnancy
Li, Liping
Tu, Jiaoqin
Jiang, Yao
Zhou, Jie
Yabe, Shinichiro
Schust, Danny J.
Effects of Lipopolysaccharide on Human First Trimester Villous Cytotrophoblast Cell Function In Vitro(1)
title Effects of Lipopolysaccharide on Human First Trimester Villous Cytotrophoblast Cell Function In Vitro(1)
title_full Effects of Lipopolysaccharide on Human First Trimester Villous Cytotrophoblast Cell Function In Vitro(1)
title_fullStr Effects of Lipopolysaccharide on Human First Trimester Villous Cytotrophoblast Cell Function In Vitro(1)
title_full_unstemmed Effects of Lipopolysaccharide on Human First Trimester Villous Cytotrophoblast Cell Function In Vitro(1)
title_short Effects of Lipopolysaccharide on Human First Trimester Villous Cytotrophoblast Cell Function In Vitro(1)
title_sort effects of lipopolysaccharide on human first trimester villous cytotrophoblast cell function in vitro(1)
topic Pregnancy
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6366481/
https://www.ncbi.nlm.nih.gov/pubmed/26700943
http://dx.doi.org/10.1095/biolreprod.115.134627
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