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Protective immunity by an engineered DNA vaccine for Mayaro virus
Mayaro virus (MAYV) of the genus alphavirus is a mosquito-transmitted emerging infectious disease that causes an acute febrile illness, rash, headaches, and nausea that may turn into incapacitating, persistent arthralgias in some victims. Since its discovery in Trinidad in 1954, cases of MAYV infect...
Autores principales: | , , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6366747/ https://www.ncbi.nlm.nih.gov/pubmed/30730897 http://dx.doi.org/10.1371/journal.pntd.0007042 |
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author | Choi, Hyeree Kudchodkar, Sagar B. Reuschel, Emma L. Asija, Kanika Borole, Piyush Ho, Michelle Wojtak, Krzysztof Reed, Charles Ramos, Stephanie Bopp, Nathen E. Aguilar, Patricia V. Weaver, Scott C. Kim, J. Joseph Humeau, Laurent Tebas, Pablo Weiner, David B. Muthumani, Kar |
author_facet | Choi, Hyeree Kudchodkar, Sagar B. Reuschel, Emma L. Asija, Kanika Borole, Piyush Ho, Michelle Wojtak, Krzysztof Reed, Charles Ramos, Stephanie Bopp, Nathen E. Aguilar, Patricia V. Weaver, Scott C. Kim, J. Joseph Humeau, Laurent Tebas, Pablo Weiner, David B. Muthumani, Kar |
author_sort | Choi, Hyeree |
collection | PubMed |
description | Mayaro virus (MAYV) of the genus alphavirus is a mosquito-transmitted emerging infectious disease that causes an acute febrile illness, rash, headaches, and nausea that may turn into incapacitating, persistent arthralgias in some victims. Since its discovery in Trinidad in 1954, cases of MAYV infection have largely been confined there and to the northern countries of South America, but recently, MAYV cases have been reported in some island nations in the Caribbean Sea. Accompanying these reports is evidence that new vectors, including Aedes spp. mosquitos, recently implicated in the global spread of Zika and chikungunya viruses, are competent for MAYV transmission, which, if true, could facilitate the spread of MAYV beyond its current range. Despite its status as an emerging virus, there are no licensed vaccines to prevent MAYV infection nor therapeutics to treat it. Here, we describe the development and testing of a novel DNA vaccine, scMAYV-E, that encodes a synthetically-designed consensus MAYV envelope sequence. In vivo electroporation-enhanced immunization of mice with this vaccine induced potent humoral responses including neutralizing antibodies as well as robust T-cell responses to multiple epitopes in the MAYV envelope. Importantly, these scMAYV-E-induced immune responses protected susceptible mice from morbidity and mortality following a MAYV challenge. |
format | Online Article Text |
id | pubmed-6366747 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-63667472019-02-22 Protective immunity by an engineered DNA vaccine for Mayaro virus Choi, Hyeree Kudchodkar, Sagar B. Reuschel, Emma L. Asija, Kanika Borole, Piyush Ho, Michelle Wojtak, Krzysztof Reed, Charles Ramos, Stephanie Bopp, Nathen E. Aguilar, Patricia V. Weaver, Scott C. Kim, J. Joseph Humeau, Laurent Tebas, Pablo Weiner, David B. Muthumani, Kar PLoS Negl Trop Dis Research Article Mayaro virus (MAYV) of the genus alphavirus is a mosquito-transmitted emerging infectious disease that causes an acute febrile illness, rash, headaches, and nausea that may turn into incapacitating, persistent arthralgias in some victims. Since its discovery in Trinidad in 1954, cases of MAYV infection have largely been confined there and to the northern countries of South America, but recently, MAYV cases have been reported in some island nations in the Caribbean Sea. Accompanying these reports is evidence that new vectors, including Aedes spp. mosquitos, recently implicated in the global spread of Zika and chikungunya viruses, are competent for MAYV transmission, which, if true, could facilitate the spread of MAYV beyond its current range. Despite its status as an emerging virus, there are no licensed vaccines to prevent MAYV infection nor therapeutics to treat it. Here, we describe the development and testing of a novel DNA vaccine, scMAYV-E, that encodes a synthetically-designed consensus MAYV envelope sequence. In vivo electroporation-enhanced immunization of mice with this vaccine induced potent humoral responses including neutralizing antibodies as well as robust T-cell responses to multiple epitopes in the MAYV envelope. Importantly, these scMAYV-E-induced immune responses protected susceptible mice from morbidity and mortality following a MAYV challenge. Public Library of Science 2019-02-07 /pmc/articles/PMC6366747/ /pubmed/30730897 http://dx.doi.org/10.1371/journal.pntd.0007042 Text en © 2019 Choi et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Choi, Hyeree Kudchodkar, Sagar B. Reuschel, Emma L. Asija, Kanika Borole, Piyush Ho, Michelle Wojtak, Krzysztof Reed, Charles Ramos, Stephanie Bopp, Nathen E. Aguilar, Patricia V. Weaver, Scott C. Kim, J. Joseph Humeau, Laurent Tebas, Pablo Weiner, David B. Muthumani, Kar Protective immunity by an engineered DNA vaccine for Mayaro virus |
title | Protective immunity by an engineered DNA vaccine for Mayaro virus |
title_full | Protective immunity by an engineered DNA vaccine for Mayaro virus |
title_fullStr | Protective immunity by an engineered DNA vaccine for Mayaro virus |
title_full_unstemmed | Protective immunity by an engineered DNA vaccine for Mayaro virus |
title_short | Protective immunity by an engineered DNA vaccine for Mayaro virus |
title_sort | protective immunity by an engineered dna vaccine for mayaro virus |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6366747/ https://www.ncbi.nlm.nih.gov/pubmed/30730897 http://dx.doi.org/10.1371/journal.pntd.0007042 |
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