Affinity-based capture and identification of protein effectors of the growth regulator ppGpp

The nucleotide ppGpp is a highly conserved regulatory molecule in prokaryotes that helps tune growth rate to nutrient availability. Despite decades of study, how ppGpp regulates growth remains poorly understood. Here, we develop and validate a capture-compound mass spectrometry approach that identifies >50 putative ppGpp targets in Escherichia coli. These targets control many key cellular processes and include 13 enzymes required for nucleotide synthesis. We demonstrate that ppGpp inhibits the de novo synthesis of all purine nucleotides by directly targeting the enzyme PurF. By solving a structure of PurF bound to ppGpp, we design a mutation that ablates ppGpp-based regulation, leading to a dysregulation of purine nucleotide synthesis following ppGpp accumulation. Collectively, our results provide new insights into ppGpp-based growth control and a nearly comprehensive set of targets for future exploration. The capture compounds developed will also now enable the rapid identification of ppGpp targets in any species, including pathogens.