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Selection of Reference Genes for Expression Analysis in Chinese Medicinal Herb Huperzia serrata
Huperzine A (HupA) is a powerful and selective inhibitor of acetylcholinesterase. It has attracted widespread attention endangering the ultimate plant sources of Lycopodiaceae family. In this study, we used Huperzia serrata, extensively used in Traditional Chinese medicine (TCM), a slow growing vasc...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Frontiers Media S.A.
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6367274/ https://www.ncbi.nlm.nih.gov/pubmed/30774594 http://dx.doi.org/10.3389/fphar.2019.00044 |
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author | Yang, Mengquan Wu, Shiwen You, Wenjing Jaisi, Amit Xiao, Youli |
author_facet | Yang, Mengquan Wu, Shiwen You, Wenjing Jaisi, Amit Xiao, Youli |
author_sort | Yang, Mengquan |
collection | PubMed |
description | Huperzine A (HupA) is a powerful and selective inhibitor of acetylcholinesterase. It has attracted widespread attention endangering the ultimate plant sources of Lycopodiaceae family. In this study, we used Huperzia serrata, extensively used in Traditional Chinese medicine (TCM), a slow growing vascular plant as the model plant of the Lycopodiaceae family to develop and validate the reference genes. We aim to use gene expression platform to understand the gene expression of different tissues and developmental stages of this medicinal herb. Eight candidate reference genes were selected based on RNA-seq data and evaluated with qRT-PCR. The expression of L/ODC and cytochrome P450s genes known for their involvement in lycopodium alkaloid biosynthesis, were also studied to validate the selected reference genes. The most stable genes were TBP, GAPDH, and their combination (TBP + GAPDH). We report for the first time the reference gene of H. serrata’s different tissues which would provide important insights into understanding their biological functions comparing other Lycopodiaceae plants and facilitate a good biopharming approach. |
format | Online Article Text |
id | pubmed-6367274 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-63672742019-02-15 Selection of Reference Genes for Expression Analysis in Chinese Medicinal Herb Huperzia serrata Yang, Mengquan Wu, Shiwen You, Wenjing Jaisi, Amit Xiao, Youli Front Pharmacol Pharmacology Huperzine A (HupA) is a powerful and selective inhibitor of acetylcholinesterase. It has attracted widespread attention endangering the ultimate plant sources of Lycopodiaceae family. In this study, we used Huperzia serrata, extensively used in Traditional Chinese medicine (TCM), a slow growing vascular plant as the model plant of the Lycopodiaceae family to develop and validate the reference genes. We aim to use gene expression platform to understand the gene expression of different tissues and developmental stages of this medicinal herb. Eight candidate reference genes were selected based on RNA-seq data and evaluated with qRT-PCR. The expression of L/ODC and cytochrome P450s genes known for their involvement in lycopodium alkaloid biosynthesis, were also studied to validate the selected reference genes. The most stable genes were TBP, GAPDH, and their combination (TBP + GAPDH). We report for the first time the reference gene of H. serrata’s different tissues which would provide important insights into understanding their biological functions comparing other Lycopodiaceae plants and facilitate a good biopharming approach. Frontiers Media S.A. 2019-02-01 /pmc/articles/PMC6367274/ /pubmed/30774594 http://dx.doi.org/10.3389/fphar.2019.00044 Text en Copyright © 2019 Yang, Wu, You, Jaisi and Xiao. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Pharmacology Yang, Mengquan Wu, Shiwen You, Wenjing Jaisi, Amit Xiao, Youli Selection of Reference Genes for Expression Analysis in Chinese Medicinal Herb Huperzia serrata |
title | Selection of Reference Genes for Expression Analysis in Chinese Medicinal Herb Huperzia serrata |
title_full | Selection of Reference Genes for Expression Analysis in Chinese Medicinal Herb Huperzia serrata |
title_fullStr | Selection of Reference Genes for Expression Analysis in Chinese Medicinal Herb Huperzia serrata |
title_full_unstemmed | Selection of Reference Genes for Expression Analysis in Chinese Medicinal Herb Huperzia serrata |
title_short | Selection of Reference Genes for Expression Analysis in Chinese Medicinal Herb Huperzia serrata |
title_sort | selection of reference genes for expression analysis in chinese medicinal herb huperzia serrata |
topic | Pharmacology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6367274/ https://www.ncbi.nlm.nih.gov/pubmed/30774594 http://dx.doi.org/10.3389/fphar.2019.00044 |
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