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RRM2 is a potential prognostic biomarker with functional significance in glioma

Glioma is one of the most common brain tumors, suggesting the importance of investigating the molecular mechanism of gliomas. We studied the roles of Ribonucleotide Reductase Regulatory Subunit M2 (RRM2) in glioma. Expressions of RRM2 are higher in glioma tissues evidenced by TCGA data, western blot...

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Detalles Bibliográficos
Autores principales: Sun, Hongzhi, Yang, Bingya, Zhang, Hao, Song, Jingwei, Zhang, Yenan, Xing, Jicheng, Yang, Zhihui, Wei, Changyong, Xu, Tuoye, Yu, Zhennan, Xu, Zhipeng, Hou, Min, Ji, Minjun, Zhang, Yansong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Ivyspring International Publisher 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6367584/
https://www.ncbi.nlm.nih.gov/pubmed/30745840
http://dx.doi.org/10.7150/ijbs.30114
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author Sun, Hongzhi
Yang, Bingya
Zhang, Hao
Song, Jingwei
Zhang, Yenan
Xing, Jicheng
Yang, Zhihui
Wei, Changyong
Xu, Tuoye
Yu, Zhennan
Xu, Zhipeng
Hou, Min
Ji, Minjun
Zhang, Yansong
author_facet Sun, Hongzhi
Yang, Bingya
Zhang, Hao
Song, Jingwei
Zhang, Yenan
Xing, Jicheng
Yang, Zhihui
Wei, Changyong
Xu, Tuoye
Yu, Zhennan
Xu, Zhipeng
Hou, Min
Ji, Minjun
Zhang, Yansong
author_sort Sun, Hongzhi
collection PubMed
description Glioma is one of the most common brain tumors, suggesting the importance of investigating the molecular mechanism of gliomas. We studied the roles of Ribonucleotide Reductase Regulatory Subunit M2 (RRM2) in glioma. Expressions of RRM2 are higher in glioma tissues evidenced by TCGA data, western blot and immunohistochemistry. RRM2 is negatively correlated with glioma patient's survival. RNA-seq showed that genes involved in apoptosis, proliferation, cell adhesion and negative regulation of signaling were up-regulated upon RNAi-mediated knock-down of RRM2. Cell phenotypes specific for stably knocking down RRM2 were determined using stable transfection in vitro. In an in vivo model, knock-down of RRM2 inhibited tumor growth and caused suppression of AKT and ERK1/2 signalings. Interfering RRM2 also down-regulated the expression of cyclin A, cyclin B1, cyclin D1, Vimentin, and N-cadherin, and elevated E-cadherin expression. Moreover, overexpression of RRM2 failed to increase the expression of cyclin B1, cyclin D1, and N-cadherin when phosphorylation of AKT and ERK1/2 was suppressed by LY294002 or PD98059. These findings indicated that RRM2 is a positive regulator of glioma progression which contributes to the migration and proliferation of glioma cells through ERK1/2 and AKT signalings and might be a novel prognostic indicator for glioma patients.
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spelling pubmed-63675842019-02-11 RRM2 is a potential prognostic biomarker with functional significance in glioma Sun, Hongzhi Yang, Bingya Zhang, Hao Song, Jingwei Zhang, Yenan Xing, Jicheng Yang, Zhihui Wei, Changyong Xu, Tuoye Yu, Zhennan Xu, Zhipeng Hou, Min Ji, Minjun Zhang, Yansong Int J Biol Sci Research Paper Glioma is one of the most common brain tumors, suggesting the importance of investigating the molecular mechanism of gliomas. We studied the roles of Ribonucleotide Reductase Regulatory Subunit M2 (RRM2) in glioma. Expressions of RRM2 are higher in glioma tissues evidenced by TCGA data, western blot and immunohistochemistry. RRM2 is negatively correlated with glioma patient's survival. RNA-seq showed that genes involved in apoptosis, proliferation, cell adhesion and negative regulation of signaling were up-regulated upon RNAi-mediated knock-down of RRM2. Cell phenotypes specific for stably knocking down RRM2 were determined using stable transfection in vitro. In an in vivo model, knock-down of RRM2 inhibited tumor growth and caused suppression of AKT and ERK1/2 signalings. Interfering RRM2 also down-regulated the expression of cyclin A, cyclin B1, cyclin D1, Vimentin, and N-cadherin, and elevated E-cadherin expression. Moreover, overexpression of RRM2 failed to increase the expression of cyclin B1, cyclin D1, and N-cadherin when phosphorylation of AKT and ERK1/2 was suppressed by LY294002 or PD98059. These findings indicated that RRM2 is a positive regulator of glioma progression which contributes to the migration and proliferation of glioma cells through ERK1/2 and AKT signalings and might be a novel prognostic indicator for glioma patients. Ivyspring International Publisher 2019-01-01 /pmc/articles/PMC6367584/ /pubmed/30745840 http://dx.doi.org/10.7150/ijbs.30114 Text en © Ivyspring International Publisher This is an open access article distributed under the terms of the Creative Commons Attribution (CC BY-NC) license (https://creativecommons.org/licenses/by-nc/4.0/). See http://ivyspring.com/terms for full terms and conditions.
spellingShingle Research Paper
Sun, Hongzhi
Yang, Bingya
Zhang, Hao
Song, Jingwei
Zhang, Yenan
Xing, Jicheng
Yang, Zhihui
Wei, Changyong
Xu, Tuoye
Yu, Zhennan
Xu, Zhipeng
Hou, Min
Ji, Minjun
Zhang, Yansong
RRM2 is a potential prognostic biomarker with functional significance in glioma
title RRM2 is a potential prognostic biomarker with functional significance in glioma
title_full RRM2 is a potential prognostic biomarker with functional significance in glioma
title_fullStr RRM2 is a potential prognostic biomarker with functional significance in glioma
title_full_unstemmed RRM2 is a potential prognostic biomarker with functional significance in glioma
title_short RRM2 is a potential prognostic biomarker with functional significance in glioma
title_sort rrm2 is a potential prognostic biomarker with functional significance in glioma
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6367584/
https://www.ncbi.nlm.nih.gov/pubmed/30745840
http://dx.doi.org/10.7150/ijbs.30114
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