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Cold Induced Antisense Transcription of FLOWERING LOCUS C in Distant Grasses
Functional conservation of RNAs between different species is a key argument for their importance. While few long non-coding RNAs are conserved at the sequence level, many long non-coding RNAs have been identified that only share a position relative to other genes. It remains largely unknown whether...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Frontiers Media S.A.
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6367677/ https://www.ncbi.nlm.nih.gov/pubmed/30774642 http://dx.doi.org/10.3389/fpls.2019.00072 |
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author | Jiao, Fuchao Pahwa, Kanchan Manning, Murray Dochy, Niklas Geuten, Koen |
author_facet | Jiao, Fuchao Pahwa, Kanchan Manning, Murray Dochy, Niklas Geuten, Koen |
author_sort | Jiao, Fuchao |
collection | PubMed |
description | Functional conservation of RNAs between different species is a key argument for their importance. While few long non-coding RNAs are conserved at the sequence level, many long non-coding RNAs have been identified that only share a position relative to other genes. It remains largely unknown whether and how these lncRNAs are conserved beyond their position. In Arabidopsis thaliana, the lncRNA COOLAIR is transcribed antisense from FLOWERING LOCUS C (FLC) in response to cold. Despite relatively low sequence similarity, the COOLAIR expression pattern and in vitro RNA secondary structure are highly conserved across the family Brassicaceae, which originated some 50 mya. It is unclear, however, whether COOLAIR functions in distantly related species such as monocots, which diverged some 150 mya. Here, we identified antisense lncRNAs from FLC homologs in various monocot species that share no sequence similarity with A. thaliana COOLAIR. Yet similar to COOLAIR, we found that BdODDSOC1 antisense (BdCOOLAIR1) and BdODDSOC2 antisense (BdCOOLAIR2) are induced by cold in a Brachypodium distachyon winter accession. Across B. distachyon accessions, the sequences of BdCOOLAIR1 and BdCOOLAIR2 are less conserved than exons but more conserved than flanking regions, suggesting a function for the transcript itself. Knock down of the BdODDSOC2 non-overlapping BdCOOLAIR2 transcript did not show a morphological phenotype, but did result in significantly higher BdODDSOC2 expression during cold, indicating that BdCOOLAIR2 performs a role in cis in the rate of BdODDSOC2 silencing. This functional similarity between eudicot and monocot species reveals ancient conservation or convergent evolution of FLC antisense transcription. Either scenario supports its functional importance. |
format | Online Article Text |
id | pubmed-6367677 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-63676772019-02-15 Cold Induced Antisense Transcription of FLOWERING LOCUS C in Distant Grasses Jiao, Fuchao Pahwa, Kanchan Manning, Murray Dochy, Niklas Geuten, Koen Front Plant Sci Plant Science Functional conservation of RNAs between different species is a key argument for their importance. While few long non-coding RNAs are conserved at the sequence level, many long non-coding RNAs have been identified that only share a position relative to other genes. It remains largely unknown whether and how these lncRNAs are conserved beyond their position. In Arabidopsis thaliana, the lncRNA COOLAIR is transcribed antisense from FLOWERING LOCUS C (FLC) in response to cold. Despite relatively low sequence similarity, the COOLAIR expression pattern and in vitro RNA secondary structure are highly conserved across the family Brassicaceae, which originated some 50 mya. It is unclear, however, whether COOLAIR functions in distantly related species such as monocots, which diverged some 150 mya. Here, we identified antisense lncRNAs from FLC homologs in various monocot species that share no sequence similarity with A. thaliana COOLAIR. Yet similar to COOLAIR, we found that BdODDSOC1 antisense (BdCOOLAIR1) and BdODDSOC2 antisense (BdCOOLAIR2) are induced by cold in a Brachypodium distachyon winter accession. Across B. distachyon accessions, the sequences of BdCOOLAIR1 and BdCOOLAIR2 are less conserved than exons but more conserved than flanking regions, suggesting a function for the transcript itself. Knock down of the BdODDSOC2 non-overlapping BdCOOLAIR2 transcript did not show a morphological phenotype, but did result in significantly higher BdODDSOC2 expression during cold, indicating that BdCOOLAIR2 performs a role in cis in the rate of BdODDSOC2 silencing. This functional similarity between eudicot and monocot species reveals ancient conservation or convergent evolution of FLC antisense transcription. Either scenario supports its functional importance. Frontiers Media S.A. 2019-02-01 /pmc/articles/PMC6367677/ /pubmed/30774642 http://dx.doi.org/10.3389/fpls.2019.00072 Text en Copyright © 2019 Jiao, Pahwa, Manning, Dochy and Geuten. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Plant Science Jiao, Fuchao Pahwa, Kanchan Manning, Murray Dochy, Niklas Geuten, Koen Cold Induced Antisense Transcription of FLOWERING LOCUS C in Distant Grasses |
title | Cold Induced Antisense Transcription of FLOWERING LOCUS C in Distant Grasses |
title_full | Cold Induced Antisense Transcription of FLOWERING LOCUS C in Distant Grasses |
title_fullStr | Cold Induced Antisense Transcription of FLOWERING LOCUS C in Distant Grasses |
title_full_unstemmed | Cold Induced Antisense Transcription of FLOWERING LOCUS C in Distant Grasses |
title_short | Cold Induced Antisense Transcription of FLOWERING LOCUS C in Distant Grasses |
title_sort | cold induced antisense transcription of flowering locus c in distant grasses |
topic | Plant Science |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6367677/ https://www.ncbi.nlm.nih.gov/pubmed/30774642 http://dx.doi.org/10.3389/fpls.2019.00072 |
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