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No evidence of involvement of E-cadherin in cell fate specification or the segregation of Epi and PrE in mouse blastocysts

During preimplantation mouse development stages, emerging pluripotent epiblast (Epi) and extraembryonic primitive endoderm (PrE) cells are first distributed in the blastocyst in a “salt-and-pepper” manner before they segregate into separate layers. As a result of segregation, PrE cells become locali...

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Autores principales: Filimonow, Katarzyna, Saiz, Nestor, Suwińska, Aneta, Wyszomirski, Tomasz, Grabarek, Joanna B., Ferretti, Elisabetta, Piliszek, Anna, Plusa, Berenika, Maleszewski, Marek
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6368326/
https://www.ncbi.nlm.nih.gov/pubmed/30735538
http://dx.doi.org/10.1371/journal.pone.0212109
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author Filimonow, Katarzyna
Saiz, Nestor
Suwińska, Aneta
Wyszomirski, Tomasz
Grabarek, Joanna B.
Ferretti, Elisabetta
Piliszek, Anna
Plusa, Berenika
Maleszewski, Marek
author_facet Filimonow, Katarzyna
Saiz, Nestor
Suwińska, Aneta
Wyszomirski, Tomasz
Grabarek, Joanna B.
Ferretti, Elisabetta
Piliszek, Anna
Plusa, Berenika
Maleszewski, Marek
author_sort Filimonow, Katarzyna
collection PubMed
description During preimplantation mouse development stages, emerging pluripotent epiblast (Epi) and extraembryonic primitive endoderm (PrE) cells are first distributed in the blastocyst in a “salt-and-pepper” manner before they segregate into separate layers. As a result of segregation, PrE cells become localised on the surface of the inner cell mass (ICM), and the Epi is enclosed by the PrE on one side and by the trophectoderm on the other. During later development, a subpopulation of PrE cells migrates away from the ICM and forms the parietal endoderm (PE), while cells remaining in contact with the Epi form the visceral endoderm (VE). Here, we asked: what are the mechanisms mediating Epi and PrE cell segregation and the subsequent VE vs PE specification? Differences in cell adhesion have been proposed; however, we demonstrate that the levels of plasma membrane-bound E-cadherin (CDH1, cadherin 1) in Epi and PrE cells only differ after the segregation of these lineages within the ICM. Moreover, manipulating E-cadherin levels did not affect lineage specification or segregation, thus failing to confirm its role during these processes. Rather, we report changes in E-cadherin localisation during later PrE-to-PE transition which are accompanied by the presence of Vimentin and Twist, supporting the hypothesis that an epithelial-to-mesenchymal transition process occurs in the mouse peri-implantation blastocyst.
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spelling pubmed-63683262019-02-22 No evidence of involvement of E-cadherin in cell fate specification or the segregation of Epi and PrE in mouse blastocysts Filimonow, Katarzyna Saiz, Nestor Suwińska, Aneta Wyszomirski, Tomasz Grabarek, Joanna B. Ferretti, Elisabetta Piliszek, Anna Plusa, Berenika Maleszewski, Marek PLoS One Research Article During preimplantation mouse development stages, emerging pluripotent epiblast (Epi) and extraembryonic primitive endoderm (PrE) cells are first distributed in the blastocyst in a “salt-and-pepper” manner before they segregate into separate layers. As a result of segregation, PrE cells become localised on the surface of the inner cell mass (ICM), and the Epi is enclosed by the PrE on one side and by the trophectoderm on the other. During later development, a subpopulation of PrE cells migrates away from the ICM and forms the parietal endoderm (PE), while cells remaining in contact with the Epi form the visceral endoderm (VE). Here, we asked: what are the mechanisms mediating Epi and PrE cell segregation and the subsequent VE vs PE specification? Differences in cell adhesion have been proposed; however, we demonstrate that the levels of plasma membrane-bound E-cadherin (CDH1, cadherin 1) in Epi and PrE cells only differ after the segregation of these lineages within the ICM. Moreover, manipulating E-cadherin levels did not affect lineage specification or segregation, thus failing to confirm its role during these processes. Rather, we report changes in E-cadherin localisation during later PrE-to-PE transition which are accompanied by the presence of Vimentin and Twist, supporting the hypothesis that an epithelial-to-mesenchymal transition process occurs in the mouse peri-implantation blastocyst. Public Library of Science 2019-02-08 /pmc/articles/PMC6368326/ /pubmed/30735538 http://dx.doi.org/10.1371/journal.pone.0212109 Text en © 2019 Filimonow et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Filimonow, Katarzyna
Saiz, Nestor
Suwińska, Aneta
Wyszomirski, Tomasz
Grabarek, Joanna B.
Ferretti, Elisabetta
Piliszek, Anna
Plusa, Berenika
Maleszewski, Marek
No evidence of involvement of E-cadherin in cell fate specification or the segregation of Epi and PrE in mouse blastocysts
title No evidence of involvement of E-cadherin in cell fate specification or the segregation of Epi and PrE in mouse blastocysts
title_full No evidence of involvement of E-cadherin in cell fate specification or the segregation of Epi and PrE in mouse blastocysts
title_fullStr No evidence of involvement of E-cadherin in cell fate specification or the segregation of Epi and PrE in mouse blastocysts
title_full_unstemmed No evidence of involvement of E-cadherin in cell fate specification or the segregation of Epi and PrE in mouse blastocysts
title_short No evidence of involvement of E-cadherin in cell fate specification or the segregation of Epi and PrE in mouse blastocysts
title_sort no evidence of involvement of e-cadherin in cell fate specification or the segregation of epi and pre in mouse blastocysts
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6368326/
https://www.ncbi.nlm.nih.gov/pubmed/30735538
http://dx.doi.org/10.1371/journal.pone.0212109
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