Plasmodium falciparum histidine-rich protein (PfHRP2 and 3) diversity in Western and Coastal Kenya

Plasmodium falciparum histidine-rich proteins 2 (PfHRP2) based RDTs are advocated in falciparum malaria-endemic regions, particularly when quality microscopy is not available. However, diversity and any deletion in the pfhrp2 and pfhrp3 genes can affect the performance of PfHRP2-based RDTs. A total...

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Autores principales: Nderu, David, Kimani, Francis, Thiong’o, Kelvin, Karanja, Evaline, Akinyi, Maureen, Too, Edwin, Chege, William, Nambati, Eva, Meyer, Christian G., Velavan, Thirumalaisamy P.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2019
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6368535/
https://www.ncbi.nlm.nih.gov/pubmed/30737461
http://dx.doi.org/10.1038/s41598-018-38175-1
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author Nderu, David
Kimani, Francis
Thiong’o, Kelvin
Karanja, Evaline
Akinyi, Maureen
Too, Edwin
Chege, William
Nambati, Eva
Meyer, Christian G.
Velavan, Thirumalaisamy P.
author_facet Nderu, David
Kimani, Francis
Thiong’o, Kelvin
Karanja, Evaline
Akinyi, Maureen
Too, Edwin
Chege, William
Nambati, Eva
Meyer, Christian G.
Velavan, Thirumalaisamy P.
author_sort Nderu, David
collection PubMed
description Plasmodium falciparum histidine-rich proteins 2 (PfHRP2) based RDTs are advocated in falciparum malaria-endemic regions, particularly when quality microscopy is not available. However, diversity and any deletion in the pfhrp2 and pfhrp3 genes can affect the performance of PfHRP2-based RDTs. A total of 400 samples collected from uncomplicated malaria cases from Kenya were investigated for the amino acid repeat profiles in exon 2 of pfhrp2 and pfhrp3 genes. In addition, PfHRP2 levels were measured in 96 individuals with uncomplicated malaria. We observed a unique distribution pattern of amino acid repeats both in the PfHRP2 and PfHRP3. 228 PfHRP2 and 124 PfHRP3 different amino acid sequences were identified. Of this, 214 (94%) PfHRP2 and 81 (65%) PfHRP3 amino acid sequences occurred only once. Thirty-nine new PfHRP2 and 20 new PfHRP3 amino acid repeat types were identified. PfHRP2 levels were not correlated with parasitemia or the number of PfHRP2 repeat types. This study shows the variability of PfHRP2, PfHRP3 and PfHRP2 concentration among uncomplicated malaria cases. These findings will be useful to understand the performance of PfHRP2-based RDTs in Kenya.
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spelling pubmed-63685352019-02-14 Plasmodium falciparum histidine-rich protein (PfHRP2 and 3) diversity in Western and Coastal Kenya Nderu, David Kimani, Francis Thiong’o, Kelvin Karanja, Evaline Akinyi, Maureen Too, Edwin Chege, William Nambati, Eva Meyer, Christian G. Velavan, Thirumalaisamy P. Sci Rep Article Plasmodium falciparum histidine-rich proteins 2 (PfHRP2) based RDTs are advocated in falciparum malaria-endemic regions, particularly when quality microscopy is not available. However, diversity and any deletion in the pfhrp2 and pfhrp3 genes can affect the performance of PfHRP2-based RDTs. A total of 400 samples collected from uncomplicated malaria cases from Kenya were investigated for the amino acid repeat profiles in exon 2 of pfhrp2 and pfhrp3 genes. In addition, PfHRP2 levels were measured in 96 individuals with uncomplicated malaria. We observed a unique distribution pattern of amino acid repeats both in the PfHRP2 and PfHRP3. 228 PfHRP2 and 124 PfHRP3 different amino acid sequences were identified. Of this, 214 (94%) PfHRP2 and 81 (65%) PfHRP3 amino acid sequences occurred only once. Thirty-nine new PfHRP2 and 20 new PfHRP3 amino acid repeat types were identified. PfHRP2 levels were not correlated with parasitemia or the number of PfHRP2 repeat types. This study shows the variability of PfHRP2, PfHRP3 and PfHRP2 concentration among uncomplicated malaria cases. These findings will be useful to understand the performance of PfHRP2-based RDTs in Kenya. Nature Publishing Group UK 2019-02-08 /pmc/articles/PMC6368535/ /pubmed/30737461 http://dx.doi.org/10.1038/s41598-018-38175-1 Text en © The Author(s) 2019 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Nderu, David
Kimani, Francis
Thiong’o, Kelvin
Karanja, Evaline
Akinyi, Maureen
Too, Edwin
Chege, William
Nambati, Eva
Meyer, Christian G.
Velavan, Thirumalaisamy P.
Plasmodium falciparum histidine-rich protein (PfHRP2 and 3) diversity in Western and Coastal Kenya
title Plasmodium falciparum histidine-rich protein (PfHRP2 and 3) diversity in Western and Coastal Kenya
title_full Plasmodium falciparum histidine-rich protein (PfHRP2 and 3) diversity in Western and Coastal Kenya
title_fullStr Plasmodium falciparum histidine-rich protein (PfHRP2 and 3) diversity in Western and Coastal Kenya
title_full_unstemmed Plasmodium falciparum histidine-rich protein (PfHRP2 and 3) diversity in Western and Coastal Kenya
title_short Plasmodium falciparum histidine-rich protein (PfHRP2 and 3) diversity in Western and Coastal Kenya
title_sort plasmodium falciparum histidine-rich protein (pfhrp2 and 3) diversity in western and coastal kenya
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6368535/
https://www.ncbi.nlm.nih.gov/pubmed/30737461
http://dx.doi.org/10.1038/s41598-018-38175-1
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