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Integrator is a key component of human telomerase RNA biogenesis

Telomeres are special DNA-protein structures that are located at the ends of linear eukaryotic chromosomes. The telomere length determines the proliferation potential of cells. Telomerase is a key component of the telomere length maintenance system. While telomerase is inactive in the majority of so...

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Autores principales: Rubtsova, M. P., Vasilkova, D. P., Moshareva, M. A., Malyavko, A. N., Meerson, M. B., Zatsepin, T. S., Naraykina, Y. V., Beletsky, A. V., Ravin, N. V., Dontsova, O. A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6368637/
https://www.ncbi.nlm.nih.gov/pubmed/30737432
http://dx.doi.org/10.1038/s41598-018-38297-6
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author Rubtsova, M. P.
Vasilkova, D. P.
Moshareva, M. A.
Malyavko, A. N.
Meerson, M. B.
Zatsepin, T. S.
Naraykina, Y. V.
Beletsky, A. V.
Ravin, N. V.
Dontsova, O. A.
author_facet Rubtsova, M. P.
Vasilkova, D. P.
Moshareva, M. A.
Malyavko, A. N.
Meerson, M. B.
Zatsepin, T. S.
Naraykina, Y. V.
Beletsky, A. V.
Ravin, N. V.
Dontsova, O. A.
author_sort Rubtsova, M. P.
collection PubMed
description Telomeres are special DNA-protein structures that are located at the ends of linear eukaryotic chromosomes. The telomere length determines the proliferation potential of cells. Telomerase is a key component of the telomere length maintenance system. While telomerase is inactive in the majority of somatic cells, its activity determines the clonogenic potential of stem cells as a resource for tissue and organism regeneration. Reactivation of telomerase occurs during the process of immortalization in the majority of cancer cells. Telomerase is a ribonucleoprotein that contains telomerase reverse transcriptase and telomerase RNA components. The RNA processing mechanism of telomerase involves exosome trimming or degradation of the primary precursor. Recent data provide evidence that the competition between the processing and decay of telomerase RNA may regulate the amount of RNA at the physiological level. We show that termination of human telomerase RNA transcription is dependent on its promoter, which engages with the multisubunit complex Integrator to interact with RNA polymerase II and terminate transcription of the human telomerase RNA gene followed by further processing.
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spelling pubmed-63686372019-02-14 Integrator is a key component of human telomerase RNA biogenesis Rubtsova, M. P. Vasilkova, D. P. Moshareva, M. A. Malyavko, A. N. Meerson, M. B. Zatsepin, T. S. Naraykina, Y. V. Beletsky, A. V. Ravin, N. V. Dontsova, O. A. Sci Rep Article Telomeres are special DNA-protein structures that are located at the ends of linear eukaryotic chromosomes. The telomere length determines the proliferation potential of cells. Telomerase is a key component of the telomere length maintenance system. While telomerase is inactive in the majority of somatic cells, its activity determines the clonogenic potential of stem cells as a resource for tissue and organism regeneration. Reactivation of telomerase occurs during the process of immortalization in the majority of cancer cells. Telomerase is a ribonucleoprotein that contains telomerase reverse transcriptase and telomerase RNA components. The RNA processing mechanism of telomerase involves exosome trimming or degradation of the primary precursor. Recent data provide evidence that the competition between the processing and decay of telomerase RNA may regulate the amount of RNA at the physiological level. We show that termination of human telomerase RNA transcription is dependent on its promoter, which engages with the multisubunit complex Integrator to interact with RNA polymerase II and terminate transcription of the human telomerase RNA gene followed by further processing. Nature Publishing Group UK 2019-02-08 /pmc/articles/PMC6368637/ /pubmed/30737432 http://dx.doi.org/10.1038/s41598-018-38297-6 Text en © The Author(s) 2019 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Rubtsova, M. P.
Vasilkova, D. P.
Moshareva, M. A.
Malyavko, A. N.
Meerson, M. B.
Zatsepin, T. S.
Naraykina, Y. V.
Beletsky, A. V.
Ravin, N. V.
Dontsova, O. A.
Integrator is a key component of human telomerase RNA biogenesis
title Integrator is a key component of human telomerase RNA biogenesis
title_full Integrator is a key component of human telomerase RNA biogenesis
title_fullStr Integrator is a key component of human telomerase RNA biogenesis
title_full_unstemmed Integrator is a key component of human telomerase RNA biogenesis
title_short Integrator is a key component of human telomerase RNA biogenesis
title_sort integrator is a key component of human telomerase rna biogenesis
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6368637/
https://www.ncbi.nlm.nih.gov/pubmed/30737432
http://dx.doi.org/10.1038/s41598-018-38297-6
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