Cargando…
The molecular characterization of fixed inversions breakpoints unveils the ancestral character of the Drosophila guanche chromosomal arrangements
Cytological studies revealed that the number of chromosomes and their organization varies across species. The increasing availability of whole genome sequences of multiple species across specific phylogenies has confirmed and greatly extended these cytological observations. In the Drosophila genus,...
Autores principales: | , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2019
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6368638/ https://www.ncbi.nlm.nih.gov/pubmed/30737415 http://dx.doi.org/10.1038/s41598-018-37121-5 |
_version_ | 1783394026256334848 |
---|---|
author | Orengo, Dorcas J. Puerma, Eva Aguadé, Montserrat |
author_facet | Orengo, Dorcas J. Puerma, Eva Aguadé, Montserrat |
author_sort | Orengo, Dorcas J. |
collection | PubMed |
description | Cytological studies revealed that the number of chromosomes and their organization varies across species. The increasing availability of whole genome sequences of multiple species across specific phylogenies has confirmed and greatly extended these cytological observations. In the Drosophila genus, the ancestral karyotype consists of five rod-like acrocentric chromosomes (Muller elements A to E) and one dot-like chromosome (element F), each exhibiting a generally conserved gene content. Chromosomal fusions and paracentric inversions are thus the major contributors, respectively, to chromosome number variation among species and to gene order variation within chromosomal element. The subobscura cluster of Drosophila consists in three species that retain the genus ancestral karyotype and differ by a reduced number of fixed inversions. Here, we have used cytological information and the D. guanche genome sequence to identify and molecularly characterize the breakpoints of inversions that became fixed since the D. guanche-D. subobscura split. Our results have led us to propose a modified version of the D. guanche cytological map of its X chromosome, and to establish that (i) most inversions became fixed in the D. subobscura lineage and (ii) the order in which the four X chromosome overlapping inversions occurred and became fixed. |
format | Online Article Text |
id | pubmed-6368638 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-63686382019-02-14 The molecular characterization of fixed inversions breakpoints unveils the ancestral character of the Drosophila guanche chromosomal arrangements Orengo, Dorcas J. Puerma, Eva Aguadé, Montserrat Sci Rep Article Cytological studies revealed that the number of chromosomes and their organization varies across species. The increasing availability of whole genome sequences of multiple species across specific phylogenies has confirmed and greatly extended these cytological observations. In the Drosophila genus, the ancestral karyotype consists of five rod-like acrocentric chromosomes (Muller elements A to E) and one dot-like chromosome (element F), each exhibiting a generally conserved gene content. Chromosomal fusions and paracentric inversions are thus the major contributors, respectively, to chromosome number variation among species and to gene order variation within chromosomal element. The subobscura cluster of Drosophila consists in three species that retain the genus ancestral karyotype and differ by a reduced number of fixed inversions. Here, we have used cytological information and the D. guanche genome sequence to identify and molecularly characterize the breakpoints of inversions that became fixed since the D. guanche-D. subobscura split. Our results have led us to propose a modified version of the D. guanche cytological map of its X chromosome, and to establish that (i) most inversions became fixed in the D. subobscura lineage and (ii) the order in which the four X chromosome overlapping inversions occurred and became fixed. Nature Publishing Group UK 2019-02-08 /pmc/articles/PMC6368638/ /pubmed/30737415 http://dx.doi.org/10.1038/s41598-018-37121-5 Text en © The Author(s) 2019 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Orengo, Dorcas J. Puerma, Eva Aguadé, Montserrat The molecular characterization of fixed inversions breakpoints unveils the ancestral character of the Drosophila guanche chromosomal arrangements |
title | The molecular characterization of fixed inversions breakpoints unveils the ancestral character of the Drosophila guanche chromosomal arrangements |
title_full | The molecular characterization of fixed inversions breakpoints unveils the ancestral character of the Drosophila guanche chromosomal arrangements |
title_fullStr | The molecular characterization of fixed inversions breakpoints unveils the ancestral character of the Drosophila guanche chromosomal arrangements |
title_full_unstemmed | The molecular characterization of fixed inversions breakpoints unveils the ancestral character of the Drosophila guanche chromosomal arrangements |
title_short | The molecular characterization of fixed inversions breakpoints unveils the ancestral character of the Drosophila guanche chromosomal arrangements |
title_sort | molecular characterization of fixed inversions breakpoints unveils the ancestral character of the drosophila guanche chromosomal arrangements |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6368638/ https://www.ncbi.nlm.nih.gov/pubmed/30737415 http://dx.doi.org/10.1038/s41598-018-37121-5 |
work_keys_str_mv | AT orengodorcasj themolecularcharacterizationoffixedinversionsbreakpointsunveilstheancestralcharacterofthedrosophilaguanchechromosomalarrangements AT puermaeva themolecularcharacterizationoffixedinversionsbreakpointsunveilstheancestralcharacterofthedrosophilaguanchechromosomalarrangements AT aguademontserrat themolecularcharacterizationoffixedinversionsbreakpointsunveilstheancestralcharacterofthedrosophilaguanchechromosomalarrangements AT orengodorcasj molecularcharacterizationoffixedinversionsbreakpointsunveilstheancestralcharacterofthedrosophilaguanchechromosomalarrangements AT puermaeva molecularcharacterizationoffixedinversionsbreakpointsunveilstheancestralcharacterofthedrosophilaguanchechromosomalarrangements AT aguademontserrat molecularcharacterizationoffixedinversionsbreakpointsunveilstheancestralcharacterofthedrosophilaguanchechromosomalarrangements |