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Single stranded (ss)RNA-mediated antiviral response against infectious laryngotracheitis virus infection
BACKGROUND: Single stranded ribonucleic acid (ssRNA) binds to toll-like receptor (TLR)7 leading to recruitment of immune cells and production of pro-inflammatory cytokines, which has been shown in mammals. In chickens, synthetic ssRNA analog, resiquimod, has been shown to elicit antiviral response a...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6368756/ https://www.ncbi.nlm.nih.gov/pubmed/30736730 http://dx.doi.org/10.1186/s12866-019-1398-6 |
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author | Abdul-Cader, Mohamed Sarjoon De Silva Senapathi, Upasama Ahmed-Hassan, Hanaa Sharif, Shayan Abdul-Careem, Mohamed Faizal |
author_facet | Abdul-Cader, Mohamed Sarjoon De Silva Senapathi, Upasama Ahmed-Hassan, Hanaa Sharif, Shayan Abdul-Careem, Mohamed Faizal |
author_sort | Abdul-Cader, Mohamed Sarjoon |
collection | PubMed |
description | BACKGROUND: Single stranded ribonucleic acid (ssRNA) binds to toll-like receptor (TLR)7 leading to recruitment of immune cells and production of pro-inflammatory cytokines, which has been shown in mammals. In chickens, synthetic ssRNA analog, resiquimod, has been shown to elicit antiviral response against infectious bursal disease virus infection. The objective of this study was to determine the innate host responses activated by the pre-hatch in ovo administration of resiquimod against infectious laryngotracheitis virus (ILTV) infection in chickens post-hatch. RESULTS: First, we observed that in ovo treatment of resiquimod at embryo day (ED) 18 increases macrophage recruitment in respiratory and gastrointestinal tissues of chicken day 1 post-hatch in addition to interleukin (IL)-1β in lungs. Second, we observed that in ovo treatment of resiquimod reduces ILTV cloacal shedding at 7 days post-infection (dpi) when challenged at day 1 post-hatch coinciding with higher macrophage recruitment. In vitro, we found that resiquimod enhances production of nitric oxide (NO) and IL-1β and not type 1 interferon (IFN) activity in avian macrophages. Although, the antiviral response against ILTV is associated with the enhanced innate immune response, it is not dependent on any of the innate immune mediators observed as has been shown in vitro using avian macrophage. CONCLUSION: This study provides insights into the mechanisms of antiviral response mediated by resiquimod, particularly against ILTV infection in chicken. |
format | Online Article Text |
id | pubmed-6368756 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-63687562019-02-15 Single stranded (ss)RNA-mediated antiviral response against infectious laryngotracheitis virus infection Abdul-Cader, Mohamed Sarjoon De Silva Senapathi, Upasama Ahmed-Hassan, Hanaa Sharif, Shayan Abdul-Careem, Mohamed Faizal BMC Microbiol Research Article BACKGROUND: Single stranded ribonucleic acid (ssRNA) binds to toll-like receptor (TLR)7 leading to recruitment of immune cells and production of pro-inflammatory cytokines, which has been shown in mammals. In chickens, synthetic ssRNA analog, resiquimod, has been shown to elicit antiviral response against infectious bursal disease virus infection. The objective of this study was to determine the innate host responses activated by the pre-hatch in ovo administration of resiquimod against infectious laryngotracheitis virus (ILTV) infection in chickens post-hatch. RESULTS: First, we observed that in ovo treatment of resiquimod at embryo day (ED) 18 increases macrophage recruitment in respiratory and gastrointestinal tissues of chicken day 1 post-hatch in addition to interleukin (IL)-1β in lungs. Second, we observed that in ovo treatment of resiquimod reduces ILTV cloacal shedding at 7 days post-infection (dpi) when challenged at day 1 post-hatch coinciding with higher macrophage recruitment. In vitro, we found that resiquimod enhances production of nitric oxide (NO) and IL-1β and not type 1 interferon (IFN) activity in avian macrophages. Although, the antiviral response against ILTV is associated with the enhanced innate immune response, it is not dependent on any of the innate immune mediators observed as has been shown in vitro using avian macrophage. CONCLUSION: This study provides insights into the mechanisms of antiviral response mediated by resiquimod, particularly against ILTV infection in chicken. BioMed Central 2019-02-08 /pmc/articles/PMC6368756/ /pubmed/30736730 http://dx.doi.org/10.1186/s12866-019-1398-6 Text en © The Author(s). 2019 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Research Article Abdul-Cader, Mohamed Sarjoon De Silva Senapathi, Upasama Ahmed-Hassan, Hanaa Sharif, Shayan Abdul-Careem, Mohamed Faizal Single stranded (ss)RNA-mediated antiviral response against infectious laryngotracheitis virus infection |
title | Single stranded (ss)RNA-mediated antiviral response against infectious laryngotracheitis virus infection |
title_full | Single stranded (ss)RNA-mediated antiviral response against infectious laryngotracheitis virus infection |
title_fullStr | Single stranded (ss)RNA-mediated antiviral response against infectious laryngotracheitis virus infection |
title_full_unstemmed | Single stranded (ss)RNA-mediated antiviral response against infectious laryngotracheitis virus infection |
title_short | Single stranded (ss)RNA-mediated antiviral response against infectious laryngotracheitis virus infection |
title_sort | single stranded (ss)rna-mediated antiviral response against infectious laryngotracheitis virus infection |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6368756/ https://www.ncbi.nlm.nih.gov/pubmed/30736730 http://dx.doi.org/10.1186/s12866-019-1398-6 |
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