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8-Isoprostane in chronic periodontitis and type II diabetes: Exploring the link

Background. Reactive oxygen species (ROS) are associated with the pathogenesis of inflammatory diseases and have a direct or indirect role in tissue damage constituting oxidative stress. ROS are also involved in impairment of β-cell function during development of diabetes, which leads to genetic abl...

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Autores principales: Koregol, Arati C, Kalburgi, Nagaraj B, kanniappa sadasivan, Sireesha, warad, shivaraj, kamat wagh, Apoorva, Thomas, Tivin, sinha, Priya
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Tabriz University of Medical Sciences 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6368953/
https://www.ncbi.nlm.nih.gov/pubmed/30774790
http://dx.doi.org/10.15171/joddd.2018.039
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author Koregol, Arati C
Kalburgi, Nagaraj B
kanniappa sadasivan, Sireesha
warad, shivaraj
kamat wagh, Apoorva
Thomas, Tivin
sinha, Priya
author_facet Koregol, Arati C
Kalburgi, Nagaraj B
kanniappa sadasivan, Sireesha
warad, shivaraj
kamat wagh, Apoorva
Thomas, Tivin
sinha, Priya
author_sort Koregol, Arati C
collection PubMed
description Background. Reactive oxygen species (ROS) are associated with the pathogenesis of inflammatory diseases and have a direct or indirect role in tissue damage constituting oxidative stress. ROS are also involved in impairment of β-cell function during development of diabetes, which leads to genetic ablation of KATP channels, triggering up-regulation of antioxidant enzymes. Several markers of lipid peroxidation, protein oxidation and DNA damage induced by ROS can be measured. Over the last decade, isoprostanes have been considered as the best markers of lipid peroxidation. The aim of this study was to determine the presence of 8-isoprostane in healthy, chronic periodontitis and chronic periodontitis subjects with type II diabetes and to find the correlation between 8-isoprostane levels among groups and with clinical parameters like gingival index, probing depth and clinical attachment levels. Methods. Ninety subjects were selected and divided into 3 groups: healthy, chronic periodontitis and chronic periodontitis subjects with type II diabetes (n=30 each). Saliva was collected from these subjects after obtaining consent and analyzed for 8-isoprostane levels using ELISA kit. Statistical analysis was performed using Kruskal-Wallis test, Mann-Whitney U test and Spearman’s correlation coefficient (P<0.001). Results. Statistically significant difference was found in the levels of 8-isoprostane between healthy, chronic periodontitis and chronic periodontitis subjects with type II diabetes and with all clinical parameters. Conclusion. 8-isoprostane can be considered as a pathophysiological marker to measure oxidative stress in periodontal diseases.
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spelling pubmed-63689532019-02-15 8-Isoprostane in chronic periodontitis and type II diabetes: Exploring the link Koregol, Arati C Kalburgi, Nagaraj B kanniappa sadasivan, Sireesha warad, shivaraj kamat wagh, Apoorva Thomas, Tivin sinha, Priya J Dent Res Dent Clin Dent Prospects Original Article Background. Reactive oxygen species (ROS) are associated with the pathogenesis of inflammatory diseases and have a direct or indirect role in tissue damage constituting oxidative stress. ROS are also involved in impairment of β-cell function during development of diabetes, which leads to genetic ablation of KATP channels, triggering up-regulation of antioxidant enzymes. Several markers of lipid peroxidation, protein oxidation and DNA damage induced by ROS can be measured. Over the last decade, isoprostanes have been considered as the best markers of lipid peroxidation. The aim of this study was to determine the presence of 8-isoprostane in healthy, chronic periodontitis and chronic periodontitis subjects with type II diabetes and to find the correlation between 8-isoprostane levels among groups and with clinical parameters like gingival index, probing depth and clinical attachment levels. Methods. Ninety subjects were selected and divided into 3 groups: healthy, chronic periodontitis and chronic periodontitis subjects with type II diabetes (n=30 each). Saliva was collected from these subjects after obtaining consent and analyzed for 8-isoprostane levels using ELISA kit. Statistical analysis was performed using Kruskal-Wallis test, Mann-Whitney U test and Spearman’s correlation coefficient (P<0.001). Results. Statistically significant difference was found in the levels of 8-isoprostane between healthy, chronic periodontitis and chronic periodontitis subjects with type II diabetes and with all clinical parameters. Conclusion. 8-isoprostane can be considered as a pathophysiological marker to measure oxidative stress in periodontal diseases. Tabriz University of Medical Sciences 2018 2018-12-19 /pmc/articles/PMC6368953/ /pubmed/30774790 http://dx.doi.org/10.15171/joddd.2018.039 Text en © 2018 Koregol et al. http://creativecommons.org/licenses/by/4.0/ This is an Open Access article published and distributed by Tabriz University of Medical Sciences under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Koregol, Arati C
Kalburgi, Nagaraj B
kanniappa sadasivan, Sireesha
warad, shivaraj
kamat wagh, Apoorva
Thomas, Tivin
sinha, Priya
8-Isoprostane in chronic periodontitis and type II diabetes: Exploring the link
title 8-Isoprostane in chronic periodontitis and type II diabetes: Exploring the link
title_full 8-Isoprostane in chronic periodontitis and type II diabetes: Exploring the link
title_fullStr 8-Isoprostane in chronic periodontitis and type II diabetes: Exploring the link
title_full_unstemmed 8-Isoprostane in chronic periodontitis and type II diabetes: Exploring the link
title_short 8-Isoprostane in chronic periodontitis and type II diabetes: Exploring the link
title_sort 8-isoprostane in chronic periodontitis and type ii diabetes: exploring the link
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6368953/
https://www.ncbi.nlm.nih.gov/pubmed/30774790
http://dx.doi.org/10.15171/joddd.2018.039
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