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Angiogenic Properties of Menstrual Stem Cells Are Impaired in Women with a History of Preeclampsia

Preeclampsia is a pregnancy-specific disorder defined by the new onset of hypertension and proteinuria after 20 weeks of gestation. Although its precise etiology is still unknown, there is evidence suggesting that it may be a consequence of impaired decidual and stromal cell function. Recently, a st...

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Autores principales: Varas-Godoy, Manuel, Acuña-Gallardo, Stephanie, Venegas-Duarte, Sebastian, Hill, Charlotte, Caceres-Verschae, Albano, Realini, Ornella, Monteiro, Lara J., Zavala, Gabriela, Khoury, Maroun, Romero, Roberto, Rice, Gregory, Illanes, Sebastian E.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6369467/
https://www.ncbi.nlm.nih.gov/pubmed/30809262
http://dx.doi.org/10.1155/2019/1916542
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author Varas-Godoy, Manuel
Acuña-Gallardo, Stephanie
Venegas-Duarte, Sebastian
Hill, Charlotte
Caceres-Verschae, Albano
Realini, Ornella
Monteiro, Lara J.
Zavala, Gabriela
Khoury, Maroun
Romero, Roberto
Rice, Gregory
Illanes, Sebastian E.
author_facet Varas-Godoy, Manuel
Acuña-Gallardo, Stephanie
Venegas-Duarte, Sebastian
Hill, Charlotte
Caceres-Verschae, Albano
Realini, Ornella
Monteiro, Lara J.
Zavala, Gabriela
Khoury, Maroun
Romero, Roberto
Rice, Gregory
Illanes, Sebastian E.
author_sort Varas-Godoy, Manuel
collection PubMed
description Preeclampsia is a pregnancy-specific disorder defined by the new onset of hypertension and proteinuria after 20 weeks of gestation. Although its precise etiology is still unknown, there is evidence suggesting that it may be a consequence of impaired decidual and stromal cell function. Recently, a stem cell population derived from endometrial tissue and isolated from menstrual effluent called menstrual stem cells (MenSCs) has been identified. MenSCs exhibit important angiogenic and inflammatory properties that may contribute to both normal and pathological complications of implantation and placentation, including preeclampsia. We hypothesized that the angiogenic and inflammatory activity of MenSCs is altered in women who have a past history of preeclampsia and that this phenotype persists postpartum. The primary outcome measures were stromal progenitor cell formation, in vitro induction of endothelial tube formation, and release of proinflammatory cytokines. MenSCs obtained from women with a previous normal or preeclamptic pregnancy displayed similar phenotypic characteristics, tri-differentiation capacity, and proliferation. MenSCs derived from women who had preeclampsia on their previous pregnancy had reduced angiogenic capacity (~30% fewer junctions and nodes, p < 0.05) and stromal progenitor cell formation (<50% measured at a serial dilution of 1 : 10.000, p < 0.05) when compared to controls. In vitro, MenSCs obtained from patients with a history of preeclampsia expressed less endoglin and secreted less VEGF but more IL-6 than controls did. These data are consistent with the hypothesis that the angiogenic and inflammatory properties of MenSCs of women with a previous pregnancy complicated by preeclampsia have reduced angiogenic capacity and are more proinflammatory than those of MenSCs of women with a previous normal pregnancy. This altered phenotype of MenSCs observed following preeclampsia could either be present before the development of the pathology, predisposing the endometrial milieu to and consequently leading to limited vascular remodeling, or be a consequence of preeclampsia itself. The former may afford opportunity for targeted therapeutic intervention; the latter, a putative biomarker for future risk of pregnancy complications.
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spelling pubmed-63694672019-02-26 Angiogenic Properties of Menstrual Stem Cells Are Impaired in Women with a History of Preeclampsia Varas-Godoy, Manuel Acuña-Gallardo, Stephanie Venegas-Duarte, Sebastian Hill, Charlotte Caceres-Verschae, Albano Realini, Ornella Monteiro, Lara J. Zavala, Gabriela Khoury, Maroun Romero, Roberto Rice, Gregory Illanes, Sebastian E. Stem Cells Int Research Article Preeclampsia is a pregnancy-specific disorder defined by the new onset of hypertension and proteinuria after 20 weeks of gestation. Although its precise etiology is still unknown, there is evidence suggesting that it may be a consequence of impaired decidual and stromal cell function. Recently, a stem cell population derived from endometrial tissue and isolated from menstrual effluent called menstrual stem cells (MenSCs) has been identified. MenSCs exhibit important angiogenic and inflammatory properties that may contribute to both normal and pathological complications of implantation and placentation, including preeclampsia. We hypothesized that the angiogenic and inflammatory activity of MenSCs is altered in women who have a past history of preeclampsia and that this phenotype persists postpartum. The primary outcome measures were stromal progenitor cell formation, in vitro induction of endothelial tube formation, and release of proinflammatory cytokines. MenSCs obtained from women with a previous normal or preeclamptic pregnancy displayed similar phenotypic characteristics, tri-differentiation capacity, and proliferation. MenSCs derived from women who had preeclampsia on their previous pregnancy had reduced angiogenic capacity (~30% fewer junctions and nodes, p < 0.05) and stromal progenitor cell formation (<50% measured at a serial dilution of 1 : 10.000, p < 0.05) when compared to controls. In vitro, MenSCs obtained from patients with a history of preeclampsia expressed less endoglin and secreted less VEGF but more IL-6 than controls did. These data are consistent with the hypothesis that the angiogenic and inflammatory properties of MenSCs of women with a previous pregnancy complicated by preeclampsia have reduced angiogenic capacity and are more proinflammatory than those of MenSCs of women with a previous normal pregnancy. This altered phenotype of MenSCs observed following preeclampsia could either be present before the development of the pathology, predisposing the endometrial milieu to and consequently leading to limited vascular remodeling, or be a consequence of preeclampsia itself. The former may afford opportunity for targeted therapeutic intervention; the latter, a putative biomarker for future risk of pregnancy complications. Hindawi 2019-01-27 /pmc/articles/PMC6369467/ /pubmed/30809262 http://dx.doi.org/10.1155/2019/1916542 Text en Copyright © 2019 Manuel Varas-Godoy et al. http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Varas-Godoy, Manuel
Acuña-Gallardo, Stephanie
Venegas-Duarte, Sebastian
Hill, Charlotte
Caceres-Verschae, Albano
Realini, Ornella
Monteiro, Lara J.
Zavala, Gabriela
Khoury, Maroun
Romero, Roberto
Rice, Gregory
Illanes, Sebastian E.
Angiogenic Properties of Menstrual Stem Cells Are Impaired in Women with a History of Preeclampsia
title Angiogenic Properties of Menstrual Stem Cells Are Impaired in Women with a History of Preeclampsia
title_full Angiogenic Properties of Menstrual Stem Cells Are Impaired in Women with a History of Preeclampsia
title_fullStr Angiogenic Properties of Menstrual Stem Cells Are Impaired in Women with a History of Preeclampsia
title_full_unstemmed Angiogenic Properties of Menstrual Stem Cells Are Impaired in Women with a History of Preeclampsia
title_short Angiogenic Properties of Menstrual Stem Cells Are Impaired in Women with a History of Preeclampsia
title_sort angiogenic properties of menstrual stem cells are impaired in women with a history of preeclampsia
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6369467/
https://www.ncbi.nlm.nih.gov/pubmed/30809262
http://dx.doi.org/10.1155/2019/1916542
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