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Thermal acclimation of photosynthetic activity and RuBisCO content in two hybrid poplar clones

The mechanistic bases of thermal acclimation of net photosynthetic rate (A(n)) are still difficult to discern, and the data sets available are scarce, particularly for hybrid poplar. In the present study, we examined the contribution of a number of biochemical and biophysical traits on thermal accli...

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Autores principales: Benomar, Lahcen, Moutaoufik, Mohamed Taha, Elferjani, Raed, Isabel, Nathalie, DesRochers, Annie, El Guellab, Ahmed, Khlifa, Rim, Idrissi Hassania, Lala Amina
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6370183/
https://www.ncbi.nlm.nih.gov/pubmed/30742644
http://dx.doi.org/10.1371/journal.pone.0206021
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author Benomar, Lahcen
Moutaoufik, Mohamed Taha
Elferjani, Raed
Isabel, Nathalie
DesRochers, Annie
El Guellab, Ahmed
Khlifa, Rim
Idrissi Hassania, Lala Amina
author_facet Benomar, Lahcen
Moutaoufik, Mohamed Taha
Elferjani, Raed
Isabel, Nathalie
DesRochers, Annie
El Guellab, Ahmed
Khlifa, Rim
Idrissi Hassania, Lala Amina
author_sort Benomar, Lahcen
collection PubMed
description The mechanistic bases of thermal acclimation of net photosynthetic rate (A(n)) are still difficult to discern, and the data sets available are scarce, particularly for hybrid poplar. In the present study, we examined the contribution of a number of biochemical and biophysical traits on thermal acclimation of A(n) for two hybrid poplar clones. We grew cuttings of Populus maximowiczii × Populus nigra (M×N) and Populus maximowiczii × Populus balsamifera (M×B) clones under two day/night temperature of 23°C/18°C and 33°C /27°C and under low and high soil nitrogen level. After ten weeks, we measured leaf RuBisCO (RAR) and RuBisCO activase (RARCA) amounts and the temperature response of A(n), dark respiration (R(d)), stomatal conductance, (g(s)), apparent maximum carboxylation rate of CO(2) (V(cmax)) and apparent photosynthetic electron transport rate (J). Results showed that a 10°C increase in growth temperature resulted in a shift in thermal optimum (T(opt)) of A(n) of 6.2±1.6°C and 8.0±1.2°C for clone M×B and M×N respectively, and an increased A(n) and g(s) at the growth temperature for clone M×B but not M×N. RuBisCO amount was increased by N level but was insensitive to growth temperature while RARCA amount and the ratio of its short to long isoform was stimulated by the warm condition for clone M×N and at low N for clone M×B. The activation energy of apparent V(cmax) and apparent J decreased under the warm condition for clone M×B and remained unchanged for clone M×N. Our study demonstrated the involvement of both RARCA, the activation energy of apparent V(cmax) and stomatal conductance in thermal acclimation of A(n).
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spelling pubmed-63701832019-02-22 Thermal acclimation of photosynthetic activity and RuBisCO content in two hybrid poplar clones Benomar, Lahcen Moutaoufik, Mohamed Taha Elferjani, Raed Isabel, Nathalie DesRochers, Annie El Guellab, Ahmed Khlifa, Rim Idrissi Hassania, Lala Amina PLoS One Research Article The mechanistic bases of thermal acclimation of net photosynthetic rate (A(n)) are still difficult to discern, and the data sets available are scarce, particularly for hybrid poplar. In the present study, we examined the contribution of a number of biochemical and biophysical traits on thermal acclimation of A(n) for two hybrid poplar clones. We grew cuttings of Populus maximowiczii × Populus nigra (M×N) and Populus maximowiczii × Populus balsamifera (M×B) clones under two day/night temperature of 23°C/18°C and 33°C /27°C and under low and high soil nitrogen level. After ten weeks, we measured leaf RuBisCO (RAR) and RuBisCO activase (RARCA) amounts and the temperature response of A(n), dark respiration (R(d)), stomatal conductance, (g(s)), apparent maximum carboxylation rate of CO(2) (V(cmax)) and apparent photosynthetic electron transport rate (J). Results showed that a 10°C increase in growth temperature resulted in a shift in thermal optimum (T(opt)) of A(n) of 6.2±1.6°C and 8.0±1.2°C for clone M×B and M×N respectively, and an increased A(n) and g(s) at the growth temperature for clone M×B but not M×N. RuBisCO amount was increased by N level but was insensitive to growth temperature while RARCA amount and the ratio of its short to long isoform was stimulated by the warm condition for clone M×N and at low N for clone M×B. The activation energy of apparent V(cmax) and apparent J decreased under the warm condition for clone M×B and remained unchanged for clone M×N. Our study demonstrated the involvement of both RARCA, the activation energy of apparent V(cmax) and stomatal conductance in thermal acclimation of A(n). Public Library of Science 2019-02-11 /pmc/articles/PMC6370183/ /pubmed/30742644 http://dx.doi.org/10.1371/journal.pone.0206021 Text en © 2019 Benomar et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Benomar, Lahcen
Moutaoufik, Mohamed Taha
Elferjani, Raed
Isabel, Nathalie
DesRochers, Annie
El Guellab, Ahmed
Khlifa, Rim
Idrissi Hassania, Lala Amina
Thermal acclimation of photosynthetic activity and RuBisCO content in two hybrid poplar clones
title Thermal acclimation of photosynthetic activity and RuBisCO content in two hybrid poplar clones
title_full Thermal acclimation of photosynthetic activity and RuBisCO content in two hybrid poplar clones
title_fullStr Thermal acclimation of photosynthetic activity and RuBisCO content in two hybrid poplar clones
title_full_unstemmed Thermal acclimation of photosynthetic activity and RuBisCO content in two hybrid poplar clones
title_short Thermal acclimation of photosynthetic activity and RuBisCO content in two hybrid poplar clones
title_sort thermal acclimation of photosynthetic activity and rubisco content in two hybrid poplar clones
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6370183/
https://www.ncbi.nlm.nih.gov/pubmed/30742644
http://dx.doi.org/10.1371/journal.pone.0206021
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