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Interference phase-contrast imaging technology without beam separation
Interferometers are widely used in science and industry to measure small displacements, changes in refractive index, and surface irregularities. In all interferometers, including phase-contrast microscopes and DICs (differential interference contrast microscopes), light from a single source is split...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6370786/ https://www.ncbi.nlm.nih.gov/pubmed/30742014 http://dx.doi.org/10.1038/s41598-018-38359-9 |
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author | Nishiwaki, Seiji Narumi, Kenji Korenaga, Tsuguhiro |
author_facet | Nishiwaki, Seiji Narumi, Kenji Korenaga, Tsuguhiro |
author_sort | Nishiwaki, Seiji |
collection | PubMed |
description | Interferometers are widely used in science and industry to measure small displacements, changes in refractive index, and surface irregularities. In all interferometers, including phase-contrast microscopes and DICs (differential interference contrast microscopes), light from a single source is split into two beams that travel along different optical paths. They are then recombined to produce interference. The fundamental operation of beam separation makes device configuration more complex and adds to the bulk of the equipment. In this study we propose a new method of observing phase-contrast images without beam separation by using self-interference inside a grating coupler structure disposed on the observation plane. We experimentally demonstrate that the self-interference principle can generate phase-contrast images using a simple configuration. From measurements using a multilevel phase plate, we confirm its phase-contrast depth resolution to approach one- tenth of a wavelength. |
format | Online Article Text |
id | pubmed-6370786 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-63707862019-02-15 Interference phase-contrast imaging technology without beam separation Nishiwaki, Seiji Narumi, Kenji Korenaga, Tsuguhiro Sci Rep Article Interferometers are widely used in science and industry to measure small displacements, changes in refractive index, and surface irregularities. In all interferometers, including phase-contrast microscopes and DICs (differential interference contrast microscopes), light from a single source is split into two beams that travel along different optical paths. They are then recombined to produce interference. The fundamental operation of beam separation makes device configuration more complex and adds to the bulk of the equipment. In this study we propose a new method of observing phase-contrast images without beam separation by using self-interference inside a grating coupler structure disposed on the observation plane. We experimentally demonstrate that the self-interference principle can generate phase-contrast images using a simple configuration. From measurements using a multilevel phase plate, we confirm its phase-contrast depth resolution to approach one- tenth of a wavelength. Nature Publishing Group UK 2019-02-11 /pmc/articles/PMC6370786/ /pubmed/30742014 http://dx.doi.org/10.1038/s41598-018-38359-9 Text en © The Author(s) 2019 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Nishiwaki, Seiji Narumi, Kenji Korenaga, Tsuguhiro Interference phase-contrast imaging technology without beam separation |
title | Interference phase-contrast imaging technology without beam separation |
title_full | Interference phase-contrast imaging technology without beam separation |
title_fullStr | Interference phase-contrast imaging technology without beam separation |
title_full_unstemmed | Interference phase-contrast imaging technology without beam separation |
title_short | Interference phase-contrast imaging technology without beam separation |
title_sort | interference phase-contrast imaging technology without beam separation |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6370786/ https://www.ncbi.nlm.nih.gov/pubmed/30742014 http://dx.doi.org/10.1038/s41598-018-38359-9 |
work_keys_str_mv | AT nishiwakiseiji interferencephasecontrastimagingtechnologywithoutbeamseparation AT narumikenji interferencephasecontrastimagingtechnologywithoutbeamseparation AT korenagatsuguhiro interferencephasecontrastimagingtechnologywithoutbeamseparation |