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HIV-1 Molecular Epidemiology, Transmission Clusters and Transmitted Drug Resistance Mutations in Central Brazil

We aimed to characterize HIV-1 molecular epidemiology and transmission clusters among heterosexual (HET) and men who have sex with men (MSM) individuals, as well as transmitted drug resistance mutations (TDRM) in Central-Western Brazil. This cross-sectional survey was conducted among 190 antiretrovi...

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Autores principales: Tanaka, Tayana Serpa Ortiz, Leite, Thaysse Ferreira, Freitas, Solange Zacalusni, Cesar, Gabriela Alves, de Rezende, Grazielli Rocha, Lindenberg, Andrea De Siqueira Campos, Guimarães, Monick Lindenmeyer, Motta-Castro, Ana Rita Coimbra
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6371026/
https://www.ncbi.nlm.nih.gov/pubmed/30804893
http://dx.doi.org/10.3389/fmicb.2019.00020
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author Tanaka, Tayana Serpa Ortiz
Leite, Thaysse Ferreira
Freitas, Solange Zacalusni
Cesar, Gabriela Alves
de Rezende, Grazielli Rocha
Lindenberg, Andrea De Siqueira Campos
Guimarães, Monick Lindenmeyer
Motta-Castro, Ana Rita Coimbra
author_facet Tanaka, Tayana Serpa Ortiz
Leite, Thaysse Ferreira
Freitas, Solange Zacalusni
Cesar, Gabriela Alves
de Rezende, Grazielli Rocha
Lindenberg, Andrea De Siqueira Campos
Guimarães, Monick Lindenmeyer
Motta-Castro, Ana Rita Coimbra
author_sort Tanaka, Tayana Serpa Ortiz
collection PubMed
description We aimed to characterize HIV-1 molecular epidemiology and transmission clusters among heterosexual (HET) and men who have sex with men (MSM) individuals, as well as transmitted drug resistance mutations (TDRM) in Central-Western Brazil. This cross-sectional survey was conducted among 190 antiretroviral naïve HIV-1 infected individuals. Proviral DNA was extracted, and nested PCR amplified partial polymerase gene (PR/RT). After sequencing, subtypes were assigned, and the sequences were analyzed for the occurrence of possible transmission networks. Calibrated Population Resistance (CPR) tool from Stanford HIV Database was used to investigate the presence of TDRM. Among 150 individuals whose samples were successfully sequenced, the most prevalent HIV-1 subtype was B, followed by recombinant forms. The occurrence of twenty transmission clusters composed by at least two sequences was verified, suggesting the existence of transmission clusters among individuals from the same or distinct sexual orientations. Intermediate level of TDRM (12%) was found in the study population, and almost half of the subjects with TDRM had more than one resistance mutation. No correlations between sexual orientation and the presence of TDRM, HIV-1 subtypes/recombinants forms were verified. Taken together, the necessity of the continuous monitoring of the TDRM to verify the importance of pre-genotyping and to delineate future strategies in primary antiretroviral therapy. Likewise, the knowledge of the HIV-1 transmission networks in Brazil would allow the implementation of effective HIV-1 prevention strategies in local settings.
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spelling pubmed-63710262019-02-25 HIV-1 Molecular Epidemiology, Transmission Clusters and Transmitted Drug Resistance Mutations in Central Brazil Tanaka, Tayana Serpa Ortiz Leite, Thaysse Ferreira Freitas, Solange Zacalusni Cesar, Gabriela Alves de Rezende, Grazielli Rocha Lindenberg, Andrea De Siqueira Campos Guimarães, Monick Lindenmeyer Motta-Castro, Ana Rita Coimbra Front Microbiol Microbiology We aimed to characterize HIV-1 molecular epidemiology and transmission clusters among heterosexual (HET) and men who have sex with men (MSM) individuals, as well as transmitted drug resistance mutations (TDRM) in Central-Western Brazil. This cross-sectional survey was conducted among 190 antiretroviral naïve HIV-1 infected individuals. Proviral DNA was extracted, and nested PCR amplified partial polymerase gene (PR/RT). After sequencing, subtypes were assigned, and the sequences were analyzed for the occurrence of possible transmission networks. Calibrated Population Resistance (CPR) tool from Stanford HIV Database was used to investigate the presence of TDRM. Among 150 individuals whose samples were successfully sequenced, the most prevalent HIV-1 subtype was B, followed by recombinant forms. The occurrence of twenty transmission clusters composed by at least two sequences was verified, suggesting the existence of transmission clusters among individuals from the same or distinct sexual orientations. Intermediate level of TDRM (12%) was found in the study population, and almost half of the subjects with TDRM had more than one resistance mutation. No correlations between sexual orientation and the presence of TDRM, HIV-1 subtypes/recombinants forms were verified. Taken together, the necessity of the continuous monitoring of the TDRM to verify the importance of pre-genotyping and to delineate future strategies in primary antiretroviral therapy. Likewise, the knowledge of the HIV-1 transmission networks in Brazil would allow the implementation of effective HIV-1 prevention strategies in local settings. Frontiers Media S.A. 2019-01-31 /pmc/articles/PMC6371026/ /pubmed/30804893 http://dx.doi.org/10.3389/fmicb.2019.00020 Text en Copyright © 2019 Tanaka, Leite, Freitas, Cesar, de Rezende, Lindenberg, Guimarães and Motta-Castro. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Tanaka, Tayana Serpa Ortiz
Leite, Thaysse Ferreira
Freitas, Solange Zacalusni
Cesar, Gabriela Alves
de Rezende, Grazielli Rocha
Lindenberg, Andrea De Siqueira Campos
Guimarães, Monick Lindenmeyer
Motta-Castro, Ana Rita Coimbra
HIV-1 Molecular Epidemiology, Transmission Clusters and Transmitted Drug Resistance Mutations in Central Brazil
title HIV-1 Molecular Epidemiology, Transmission Clusters and Transmitted Drug Resistance Mutations in Central Brazil
title_full HIV-1 Molecular Epidemiology, Transmission Clusters and Transmitted Drug Resistance Mutations in Central Brazil
title_fullStr HIV-1 Molecular Epidemiology, Transmission Clusters and Transmitted Drug Resistance Mutations in Central Brazil
title_full_unstemmed HIV-1 Molecular Epidemiology, Transmission Clusters and Transmitted Drug Resistance Mutations in Central Brazil
title_short HIV-1 Molecular Epidemiology, Transmission Clusters and Transmitted Drug Resistance Mutations in Central Brazil
title_sort hiv-1 molecular epidemiology, transmission clusters and transmitted drug resistance mutations in central brazil
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6371026/
https://www.ncbi.nlm.nih.gov/pubmed/30804893
http://dx.doi.org/10.3389/fmicb.2019.00020
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