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Genome-wide transcriptome analysis reveals the molecular mechanism of high temperature-induced floral abortion in Litchi chinensis

BACKGROUND: Warm winter and hot spring attributed to global warming affected floral development and may induce floral abortion, resulted in poor flowering in litchi. To identify genes potentially involved in litchi floral abortion, six RNA-sequencing (RNA-Seq) libraries of the developing panicles (D...

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Autores principales: Liu, Hao, Wang, Congcong, Chen, Houbin, Zhou, Biyan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6371443/
https://www.ncbi.nlm.nih.gov/pubmed/30744557
http://dx.doi.org/10.1186/s12864-019-5493-8
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author Liu, Hao
Wang, Congcong
Chen, Houbin
Zhou, Biyan
author_facet Liu, Hao
Wang, Congcong
Chen, Houbin
Zhou, Biyan
author_sort Liu, Hao
collection PubMed
description BACKGROUND: Warm winter and hot spring attributed to global warming affected floral development and may induce floral abortion, resulted in poor flowering in litchi. To identify genes potentially involved in litchi floral abortion, six RNA-sequencing (RNA-Seq) libraries of the developing panicles (DPs) under low temperature (LT) conditions and the shrinking panicles (SPs) under high temperature (HT) conditions were constructed. RESULTS: 3.07–8.97 × 10(6) clean reads were generated. Digital expression of the DPs with that of the SPs was compared. As a result, 1320 up-regulated and 981 down-regulated differentially expressed genes (DEGs) were identified. From the enriched GO-term, 54 temperature responsive DEGs, 23 hormone homeostasis- or biosynthesis-related DEGs, 137 hormone signal transduction or responsive DEGs, and 18 flowering-related DEGs were identified. Partial Least Squares Structural Equation Modeling (PLS-SEM) analysis indicated that the effects of hormone-related DEGs on NACs, MYBs, WRKYs were stronger than that on flowering-related DEGs. Expression pattern analysis of the inflorescence in ‘Nuomici’ and ‘Huaizhi’ under LT and HT conditions showed that genes homologous to AIL6 (LcAIL6), LHY (LcLHY), MED16 (LcMED16), SKIP20 (LcSKIP20), POD20 (LcPOD20) in the two cultivars had similar expression trends. CONCLUSION: This study elucidated the transcriptome in the HT-induced floral abortion and identified key genes involved in the process. NACs, MYBs, WRKYs may act as central players involved in the HT-induced floral abortion underlying hormonal control. Increased transcript in LcLHY, LcMED16, LcSKIP20, LcPOD20 and decreased transcript in LcAIL6 might be related to the inhibition of floral development. Our studies provided potential genes for the future molecular breeding of new cultivars that can reduce floral abortion under warm climates, and a novel clue to reveal the relationship of biological processes based on the RNA-seq data using PLS-SEM. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s12864-019-5493-8) contains supplementary material, which is available to authorized users.
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spelling pubmed-63714432019-02-21 Genome-wide transcriptome analysis reveals the molecular mechanism of high temperature-induced floral abortion in Litchi chinensis Liu, Hao Wang, Congcong Chen, Houbin Zhou, Biyan BMC Genomics Research Article BACKGROUND: Warm winter and hot spring attributed to global warming affected floral development and may induce floral abortion, resulted in poor flowering in litchi. To identify genes potentially involved in litchi floral abortion, six RNA-sequencing (RNA-Seq) libraries of the developing panicles (DPs) under low temperature (LT) conditions and the shrinking panicles (SPs) under high temperature (HT) conditions were constructed. RESULTS: 3.07–8.97 × 10(6) clean reads were generated. Digital expression of the DPs with that of the SPs was compared. As a result, 1320 up-regulated and 981 down-regulated differentially expressed genes (DEGs) were identified. From the enriched GO-term, 54 temperature responsive DEGs, 23 hormone homeostasis- or biosynthesis-related DEGs, 137 hormone signal transduction or responsive DEGs, and 18 flowering-related DEGs were identified. Partial Least Squares Structural Equation Modeling (PLS-SEM) analysis indicated that the effects of hormone-related DEGs on NACs, MYBs, WRKYs were stronger than that on flowering-related DEGs. Expression pattern analysis of the inflorescence in ‘Nuomici’ and ‘Huaizhi’ under LT and HT conditions showed that genes homologous to AIL6 (LcAIL6), LHY (LcLHY), MED16 (LcMED16), SKIP20 (LcSKIP20), POD20 (LcPOD20) in the two cultivars had similar expression trends. CONCLUSION: This study elucidated the transcriptome in the HT-induced floral abortion and identified key genes involved in the process. NACs, MYBs, WRKYs may act as central players involved in the HT-induced floral abortion underlying hormonal control. Increased transcript in LcLHY, LcMED16, LcSKIP20, LcPOD20 and decreased transcript in LcAIL6 might be related to the inhibition of floral development. Our studies provided potential genes for the future molecular breeding of new cultivars that can reduce floral abortion under warm climates, and a novel clue to reveal the relationship of biological processes based on the RNA-seq data using PLS-SEM. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s12864-019-5493-8) contains supplementary material, which is available to authorized users. BioMed Central 2019-02-11 /pmc/articles/PMC6371443/ /pubmed/30744557 http://dx.doi.org/10.1186/s12864-019-5493-8 Text en © The Author(s). 2019 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research Article
Liu, Hao
Wang, Congcong
Chen, Houbin
Zhou, Biyan
Genome-wide transcriptome analysis reveals the molecular mechanism of high temperature-induced floral abortion in Litchi chinensis
title Genome-wide transcriptome analysis reveals the molecular mechanism of high temperature-induced floral abortion in Litchi chinensis
title_full Genome-wide transcriptome analysis reveals the molecular mechanism of high temperature-induced floral abortion in Litchi chinensis
title_fullStr Genome-wide transcriptome analysis reveals the molecular mechanism of high temperature-induced floral abortion in Litchi chinensis
title_full_unstemmed Genome-wide transcriptome analysis reveals the molecular mechanism of high temperature-induced floral abortion in Litchi chinensis
title_short Genome-wide transcriptome analysis reveals the molecular mechanism of high temperature-induced floral abortion in Litchi chinensis
title_sort genome-wide transcriptome analysis reveals the molecular mechanism of high temperature-induced floral abortion in litchi chinensis
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6371443/
https://www.ncbi.nlm.nih.gov/pubmed/30744557
http://dx.doi.org/10.1186/s12864-019-5493-8
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