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Influence of temperature on development, reproduction and regeneration in the flatworm model organism, Macrostomum lignano
BACKGROUND: The free-living marine flatworm Macrostomum lignano is a powerful model organism for use in studying mechanisms of regeneration and stem cell regulation due to its combination of biological and experimental properties, including the availability of transgenesis methods, which is unique a...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6371448/ https://www.ncbi.nlm.nih.gov/pubmed/30805201 http://dx.doi.org/10.1186/s40851-019-0122-6 |
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author | Wudarski, Jakub Ustyantsev, Kirill Glazenburg, Lisa Berezikov, Eugene |
author_facet | Wudarski, Jakub Ustyantsev, Kirill Glazenburg, Lisa Berezikov, Eugene |
author_sort | Wudarski, Jakub |
collection | PubMed |
description | BACKGROUND: The free-living marine flatworm Macrostomum lignano is a powerful model organism for use in studying mechanisms of regeneration and stem cell regulation due to its combination of biological and experimental properties, including the availability of transgenesis methods, which is unique among flatworm models. However, due to its relatively recent introduction in research, many aspects of this animal’s biology remain unknown. One such question is the influence of culture temperature on Macrostomum biology. RESULTS: We systematically investigated how different culture temperatures affect development time, reproduction rate, regeneration, heat shock response, and gene knockdown efficiency by RNA interference (RNAi) in M. lignano. We used marker transgenic lines to accurately measure the regeneration endpoint, and to establish the stress response threshold for temperature shock. We found that compared to the culture temperature of 20 °C commonly used for M. lignano, temperatures of 25 °C–30 °C substantially increase the speed of development and regeneration, lead to faster manifestation of RNAi phenotypes, and increase reproduction rate without detectable negative consequences for the animal, while temperatures above 30 °C elicit a heat shock response. CONCLUSIONS: We show that altering temperature conditions can be used to reduce the time required to establish M. lignano cultures, perform RNAi experiments, store important lines, and optimize microinjection procedures for transgenesis. These findings will help to optimize the design of experiments in M. lignano, and thus facilitate future research using this model organism. |
format | Online Article Text |
id | pubmed-6371448 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-63714482019-02-25 Influence of temperature on development, reproduction and regeneration in the flatworm model organism, Macrostomum lignano Wudarski, Jakub Ustyantsev, Kirill Glazenburg, Lisa Berezikov, Eugene Zoological Lett Research Article BACKGROUND: The free-living marine flatworm Macrostomum lignano is a powerful model organism for use in studying mechanisms of regeneration and stem cell regulation due to its combination of biological and experimental properties, including the availability of transgenesis methods, which is unique among flatworm models. However, due to its relatively recent introduction in research, many aspects of this animal’s biology remain unknown. One such question is the influence of culture temperature on Macrostomum biology. RESULTS: We systematically investigated how different culture temperatures affect development time, reproduction rate, regeneration, heat shock response, and gene knockdown efficiency by RNA interference (RNAi) in M. lignano. We used marker transgenic lines to accurately measure the regeneration endpoint, and to establish the stress response threshold for temperature shock. We found that compared to the culture temperature of 20 °C commonly used for M. lignano, temperatures of 25 °C–30 °C substantially increase the speed of development and regeneration, lead to faster manifestation of RNAi phenotypes, and increase reproduction rate without detectable negative consequences for the animal, while temperatures above 30 °C elicit a heat shock response. CONCLUSIONS: We show that altering temperature conditions can be used to reduce the time required to establish M. lignano cultures, perform RNAi experiments, store important lines, and optimize microinjection procedures for transgenesis. These findings will help to optimize the design of experiments in M. lignano, and thus facilitate future research using this model organism. BioMed Central 2019-02-12 /pmc/articles/PMC6371448/ /pubmed/30805201 http://dx.doi.org/10.1186/s40851-019-0122-6 Text en © The Author(s). 2019 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Research Article Wudarski, Jakub Ustyantsev, Kirill Glazenburg, Lisa Berezikov, Eugene Influence of temperature on development, reproduction and regeneration in the flatworm model organism, Macrostomum lignano |
title | Influence of temperature on development, reproduction and regeneration in the flatworm model organism, Macrostomum lignano |
title_full | Influence of temperature on development, reproduction and regeneration in the flatworm model organism, Macrostomum lignano |
title_fullStr | Influence of temperature on development, reproduction and regeneration in the flatworm model organism, Macrostomum lignano |
title_full_unstemmed | Influence of temperature on development, reproduction and regeneration in the flatworm model organism, Macrostomum lignano |
title_short | Influence of temperature on development, reproduction and regeneration in the flatworm model organism, Macrostomum lignano |
title_sort | influence of temperature on development, reproduction and regeneration in the flatworm model organism, macrostomum lignano |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6371448/ https://www.ncbi.nlm.nih.gov/pubmed/30805201 http://dx.doi.org/10.1186/s40851-019-0122-6 |
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