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DNA methylation-mediated silencing of miR-204 is a potential prognostic marker for papillary thyroid carcinoma

BACKGROUND: Papillary thyroid carcinoma (PTC) is the most common endocrine malignancy and its incidence has increased over the last few decades. The molecular mechanisms underlying PTC tumorigenesis and progression are still unclear. PATIENTS AND METHODS: The microRNA (miRNA) expression patterns of...

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Autores principales: Xia, Fada, Wang, Wenlong, Jiang, Bo, Chen, Yong, Li, Xinying
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Dove Medical Press 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6371936/
https://www.ncbi.nlm.nih.gov/pubmed/30799952
http://dx.doi.org/10.2147/CMAR.S184566
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author Xia, Fada
Wang, Wenlong
Jiang, Bo
Chen, Yong
Li, Xinying
author_facet Xia, Fada
Wang, Wenlong
Jiang, Bo
Chen, Yong
Li, Xinying
author_sort Xia, Fada
collection PubMed
description BACKGROUND: Papillary thyroid carcinoma (PTC) is the most common endocrine malignancy and its incidence has increased over the last few decades. The molecular mechanisms underlying PTC tumorigenesis and progression are still unclear. PATIENTS AND METHODS: The microRNA (miRNA) expression patterns of PTC were revealed by miRNA microarray analysis and validated with The Cancer Genome Atlas (TCGA) data. Promoter DNA methylation rates of miR-204 were analyzed by Agena Methylation MassAR-RAY analysis and validated with TCGA data. The underlying molecular mechanisms of miR-204 involved in PTC were studied by bioinformatics analyses. RESULTS: A total of 181 differentially expressed miRNAs (89 downregulated and 92 upregulated miRNAs) between PTC and normal tissues were detected in this study. We identified miR-204 as one of the most significantly downregulated miRNAs in PTC. Downregulation of miR-204 was related to PTC extrathyroidal extension, high T-stage, lymph metastasis, BRAF V600E mutation, and aggressive tall cell variant. The Agena MassARRAY results indicated that 12 CpG sites located at the promoter of miR-204 were hypermethylated in PTC tissues compared to normal tissues. The promoter methylation rates of miR-204 in PTC were negatively correlated with the expression levels of miR-204 and its host gene TRPM3. Downregulated miR-204 expression was related to several important pathways and mechanisms involved in tumorigenesis and progression. CONCLUSION: Promoter DNA methylation-silenced miR-204 could serve as a potential diagnostic biomarker of PTC. Downregulation of miR-204 may play an important role in PTC via its involvement in many tumor-related pathways. Novel target genes and putative mechanisms of miR-204 in PTC need to be further validated.
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spelling pubmed-63719362019-02-22 DNA methylation-mediated silencing of miR-204 is a potential prognostic marker for papillary thyroid carcinoma Xia, Fada Wang, Wenlong Jiang, Bo Chen, Yong Li, Xinying Cancer Manag Res Original Research BACKGROUND: Papillary thyroid carcinoma (PTC) is the most common endocrine malignancy and its incidence has increased over the last few decades. The molecular mechanisms underlying PTC tumorigenesis and progression are still unclear. PATIENTS AND METHODS: The microRNA (miRNA) expression patterns of PTC were revealed by miRNA microarray analysis and validated with The Cancer Genome Atlas (TCGA) data. Promoter DNA methylation rates of miR-204 were analyzed by Agena Methylation MassAR-RAY analysis and validated with TCGA data. The underlying molecular mechanisms of miR-204 involved in PTC were studied by bioinformatics analyses. RESULTS: A total of 181 differentially expressed miRNAs (89 downregulated and 92 upregulated miRNAs) between PTC and normal tissues were detected in this study. We identified miR-204 as one of the most significantly downregulated miRNAs in PTC. Downregulation of miR-204 was related to PTC extrathyroidal extension, high T-stage, lymph metastasis, BRAF V600E mutation, and aggressive tall cell variant. The Agena MassARRAY results indicated that 12 CpG sites located at the promoter of miR-204 were hypermethylated in PTC tissues compared to normal tissues. The promoter methylation rates of miR-204 in PTC were negatively correlated with the expression levels of miR-204 and its host gene TRPM3. Downregulated miR-204 expression was related to several important pathways and mechanisms involved in tumorigenesis and progression. CONCLUSION: Promoter DNA methylation-silenced miR-204 could serve as a potential diagnostic biomarker of PTC. Downregulation of miR-204 may play an important role in PTC via its involvement in many tumor-related pathways. Novel target genes and putative mechanisms of miR-204 in PTC need to be further validated. Dove Medical Press 2019-02-08 /pmc/articles/PMC6371936/ /pubmed/30799952 http://dx.doi.org/10.2147/CMAR.S184566 Text en © 2019 Xia et al. This work is published and licensed by Dove Medical Press Limited The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed.
spellingShingle Original Research
Xia, Fada
Wang, Wenlong
Jiang, Bo
Chen, Yong
Li, Xinying
DNA methylation-mediated silencing of miR-204 is a potential prognostic marker for papillary thyroid carcinoma
title DNA methylation-mediated silencing of miR-204 is a potential prognostic marker for papillary thyroid carcinoma
title_full DNA methylation-mediated silencing of miR-204 is a potential prognostic marker for papillary thyroid carcinoma
title_fullStr DNA methylation-mediated silencing of miR-204 is a potential prognostic marker for papillary thyroid carcinoma
title_full_unstemmed DNA methylation-mediated silencing of miR-204 is a potential prognostic marker for papillary thyroid carcinoma
title_short DNA methylation-mediated silencing of miR-204 is a potential prognostic marker for papillary thyroid carcinoma
title_sort dna methylation-mediated silencing of mir-204 is a potential prognostic marker for papillary thyroid carcinoma
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6371936/
https://www.ncbi.nlm.nih.gov/pubmed/30799952
http://dx.doi.org/10.2147/CMAR.S184566
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