TROY interacts with RKIP to promote glioma development

TROY is a component of the Nogo receptor complex and plays the key role in neuronal survival, migration, and differentiation. Here, we show the up-regulation of TROY in human glioma tissues and cells. Inhibition of TROY expression slowed glioma development in vivo and in vitro. Raf kinase inhibitor...

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Autores principales: Liu, Xiujie, Bao, Yinghui, Meng, Wei, Yang, Ping, An, Yi, Ma, Jie, Tang, Yujie, Liu, Zhigang, Lu, Yan, Zhou, Jianfeng, Zhang, Yong, Feng, Jifeng, Gao, Xiaofei, Su, Zhida, Pu, Yingyan, He, Cheng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2018
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6372479/
https://www.ncbi.nlm.nih.gov/pubmed/30337686
http://dx.doi.org/10.1038/s41388-018-0503-x
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author Liu, Xiujie
Bao, Yinghui
Meng, Wei
Yang, Ping
An, Yi
Ma, Jie
Tang, Yujie
Liu, Zhigang
Lu, Yan
Zhou, Jianfeng
Zhang, Yong
Feng, Jifeng
Gao, Xiaofei
Su, Zhida
Pu, Yingyan
He, Cheng
author_facet Liu, Xiujie
Bao, Yinghui
Meng, Wei
Yang, Ping
An, Yi
Ma, Jie
Tang, Yujie
Liu, Zhigang
Lu, Yan
Zhou, Jianfeng
Zhang, Yong
Feng, Jifeng
Gao, Xiaofei
Su, Zhida
Pu, Yingyan
He, Cheng
author_sort Liu, Xiujie
collection PubMed
description TROY is a component of the Nogo receptor complex and plays the key role in neuronal survival, migration, and differentiation. Here, we show the up-regulation of TROY in human glioma tissues and cells. Inhibition of TROY expression slowed glioma development in vivo and in vitro. Raf kinase inhibitor (RKIP) was found to interact with TROY. The physical interaction of TROY/RKIP was confirmed via co-immunoprecipitation (co-IP) assays. Furthermore, we found that the TROY/RKIP interaction was enhanced by fetal bovine serum (FBS) exposure, and TROY knockdown also led to down-regulation of NF-κB. Finally, disruption of the TROY/RKIP interaction using the TAT-TROY (234–371 aa) protein reduced the glioma development in xenografted mice. This suggests the TROY/RKIP interaction is a potential target for therapy of gliomas.
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spelling pubmed-63724792019-02-14 TROY interacts with RKIP to promote glioma development Liu, Xiujie Bao, Yinghui Meng, Wei Yang, Ping An, Yi Ma, Jie Tang, Yujie Liu, Zhigang Lu, Yan Zhou, Jianfeng Zhang, Yong Feng, Jifeng Gao, Xiaofei Su, Zhida Pu, Yingyan He, Cheng Oncogene Article TROY is a component of the Nogo receptor complex and plays the key role in neuronal survival, migration, and differentiation. Here, we show the up-regulation of TROY in human glioma tissues and cells. Inhibition of TROY expression slowed glioma development in vivo and in vitro. Raf kinase inhibitor (RKIP) was found to interact with TROY. The physical interaction of TROY/RKIP was confirmed via co-immunoprecipitation (co-IP) assays. Furthermore, we found that the TROY/RKIP interaction was enhanced by fetal bovine serum (FBS) exposure, and TROY knockdown also led to down-regulation of NF-κB. Finally, disruption of the TROY/RKIP interaction using the TAT-TROY (234–371 aa) protein reduced the glioma development in xenografted mice. This suggests the TROY/RKIP interaction is a potential target for therapy of gliomas. Nature Publishing Group UK 2018-10-18 2019 /pmc/articles/PMC6372479/ /pubmed/30337686 http://dx.doi.org/10.1038/s41388-018-0503-x Text en © The Author(s) 2018 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Liu, Xiujie
Bao, Yinghui
Meng, Wei
Yang, Ping
An, Yi
Ma, Jie
Tang, Yujie
Liu, Zhigang
Lu, Yan
Zhou, Jianfeng
Zhang, Yong
Feng, Jifeng
Gao, Xiaofei
Su, Zhida
Pu, Yingyan
He, Cheng
TROY interacts with RKIP to promote glioma development
title TROY interacts with RKIP to promote glioma development
title_full TROY interacts with RKIP to promote glioma development
title_fullStr TROY interacts with RKIP to promote glioma development
title_full_unstemmed TROY interacts with RKIP to promote glioma development
title_short TROY interacts with RKIP to promote glioma development
title_sort troy interacts with rkip to promote glioma development
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6372479/
https://www.ncbi.nlm.nih.gov/pubmed/30337686
http://dx.doi.org/10.1038/s41388-018-0503-x
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