Cargando…

Quercetin glycosides prevent dexamethasone-induced muscle atrophy in mice

Although quercetin has numerous biological benefits, including preventing muscle atrophy due to disuse, no reports have been published to date about the preventive effects and molecular mechanisms underlying drug-induced muscle atrophy. Highly soluble and bioavailable quercetin glycosides (QGs) were...

Descripción completa

Detalles Bibliográficos
Autores principales: Otsuka, Yuta, Egawa, Kahori, Kanzaki, Noriyuki, Izumo, Takayuki, Rogi, Tomohiro, Shibata, Hiroshi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6372881/
https://www.ncbi.nlm.nih.gov/pubmed/30805562
http://dx.doi.org/10.1016/j.bbrep.2019.100618
_version_ 1783394853671927808
author Otsuka, Yuta
Egawa, Kahori
Kanzaki, Noriyuki
Izumo, Takayuki
Rogi, Tomohiro
Shibata, Hiroshi
author_facet Otsuka, Yuta
Egawa, Kahori
Kanzaki, Noriyuki
Izumo, Takayuki
Rogi, Tomohiro
Shibata, Hiroshi
author_sort Otsuka, Yuta
collection PubMed
description Although quercetin has numerous biological benefits, including preventing muscle atrophy due to disuse, no reports have been published to date about the preventive effects and molecular mechanisms underlying drug-induced muscle atrophy. Highly soluble and bioavailable quercetin glycosides (QGs) were used to examine the inhibition of dexamethasone (DEX)-induced muscle atrophy in vivo. Male BALB/cCrSlc mice were treated with or without QGs for 7 days ad libitum, followed by addition of DEX to their drinking water for a further 7 days. The weight of gastrocnemius (GM) adjusted by body weight was significantly decreased on day 7 after DEX treatment. DEX-induced decrease of GM weight was improved by QG co-administration on day 7. The mRNA levels of muscle atrophy-related genes in the gastrocnemius were significantly lowered by QGs on day 1. In particular, the expression of myostatin, a master regulator of muscle mass homeostasis, was suppressed to that of the control level. In murine C2C12 myotubes, quercetin elevated the phosphorylation of Akt, which are downstream of the myostatin pathway, as well as expression of atrogenes. We demonstrated the protective effect of QGs in DEX-induced muscle atrophy, which might depend on the suppression of myostatin signaling.
format Online
Article
Text
id pubmed-6372881
institution National Center for Biotechnology Information
language English
publishDate 2019
publisher Elsevier
record_format MEDLINE/PubMed
spelling pubmed-63728812019-02-25 Quercetin glycosides prevent dexamethasone-induced muscle atrophy in mice Otsuka, Yuta Egawa, Kahori Kanzaki, Noriyuki Izumo, Takayuki Rogi, Tomohiro Shibata, Hiroshi Biochem Biophys Rep Research Article Although quercetin has numerous biological benefits, including preventing muscle atrophy due to disuse, no reports have been published to date about the preventive effects and molecular mechanisms underlying drug-induced muscle atrophy. Highly soluble and bioavailable quercetin glycosides (QGs) were used to examine the inhibition of dexamethasone (DEX)-induced muscle atrophy in vivo. Male BALB/cCrSlc mice were treated with or without QGs for 7 days ad libitum, followed by addition of DEX to their drinking water for a further 7 days. The weight of gastrocnemius (GM) adjusted by body weight was significantly decreased on day 7 after DEX treatment. DEX-induced decrease of GM weight was improved by QG co-administration on day 7. The mRNA levels of muscle atrophy-related genes in the gastrocnemius were significantly lowered by QGs on day 1. In particular, the expression of myostatin, a master regulator of muscle mass homeostasis, was suppressed to that of the control level. In murine C2C12 myotubes, quercetin elevated the phosphorylation of Akt, which are downstream of the myostatin pathway, as well as expression of atrogenes. We demonstrated the protective effect of QGs in DEX-induced muscle atrophy, which might depend on the suppression of myostatin signaling. Elsevier 2019-02-11 /pmc/articles/PMC6372881/ /pubmed/30805562 http://dx.doi.org/10.1016/j.bbrep.2019.100618 Text en © 2019 The Authors http://creativecommons.org/licenses/by/4.0/ This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Research Article
Otsuka, Yuta
Egawa, Kahori
Kanzaki, Noriyuki
Izumo, Takayuki
Rogi, Tomohiro
Shibata, Hiroshi
Quercetin glycosides prevent dexamethasone-induced muscle atrophy in mice
title Quercetin glycosides prevent dexamethasone-induced muscle atrophy in mice
title_full Quercetin glycosides prevent dexamethasone-induced muscle atrophy in mice
title_fullStr Quercetin glycosides prevent dexamethasone-induced muscle atrophy in mice
title_full_unstemmed Quercetin glycosides prevent dexamethasone-induced muscle atrophy in mice
title_short Quercetin glycosides prevent dexamethasone-induced muscle atrophy in mice
title_sort quercetin glycosides prevent dexamethasone-induced muscle atrophy in mice
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6372881/
https://www.ncbi.nlm.nih.gov/pubmed/30805562
http://dx.doi.org/10.1016/j.bbrep.2019.100618
work_keys_str_mv AT otsukayuta quercetinglycosidespreventdexamethasoneinducedmuscleatrophyinmice
AT egawakahori quercetinglycosidespreventdexamethasoneinducedmuscleatrophyinmice
AT kanzakinoriyuki quercetinglycosidespreventdexamethasoneinducedmuscleatrophyinmice
AT izumotakayuki quercetinglycosidespreventdexamethasoneinducedmuscleatrophyinmice
AT rogitomohiro quercetinglycosidespreventdexamethasoneinducedmuscleatrophyinmice
AT shibatahiroshi quercetinglycosidespreventdexamethasoneinducedmuscleatrophyinmice