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Low let-7d and high miR-205 expression levels positively influence HNSCC patient outcome
INTRODUCTION: Head and neck squamous carcinoma (HNSCC) is one of the most invasive types of cancer with high mortality. A previous study has indicated that low levels of let-7d and miR-205 in HNSCC patients are correlated with poor survival. Let-7d and miR-205 are tumor suppressors and regulators of...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6373017/ https://www.ncbi.nlm.nih.gov/pubmed/30755200 http://dx.doi.org/10.1186/s12929-019-0511-3 |
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author | Kolenda, Tomasz Guglas, Kacper Teresiak, Anna Bliźniak, Renata Lamperska, Katarzyna |
author_facet | Kolenda, Tomasz Guglas, Kacper Teresiak, Anna Bliźniak, Renata Lamperska, Katarzyna |
author_sort | Kolenda, Tomasz |
collection | PubMed |
description | INTRODUCTION: Head and neck squamous carcinoma (HNSCC) is one of the most invasive types of cancer with high mortality. A previous study has indicated that low levels of let-7d and miR-205 in HNSCC patients are correlated with poor survival. Let-7d and miR-205 are tumor suppressors and regulators of epithelial-to-mesenchymal transition (EMT). However, it is unclear if let-7d and miR-205 together influence cancer cells. AIM: To determine if let-7d and miR-205 expression levels influence HNSCC patient outcome. METHODS: The TCGA expression data for let-7d, miR-205 and their targets as well as clinical data were downloaded from cBioPortal and starBase v2.0 for 307 patients. The expression levels of let-7d and miR-205 were verified according to clinicopathological parameters. The let-7d and miR-205 high- and low-expression groups as well as disease-free survival (DFS), overall survival (OS) and expression levels of genes related to EMT, cancer stem cells, metastasis, cell cycle, drug response and irradiation response were investigated. RESULTS: Let-7d and miR-205 were frequently upregulated in HNSCC compared to normal samples, and ROC analysis showed high discrimination ability for let-7d and miR-205 (area 0.7369 and 0.7739, respectively; p < 0.0001). Differences between expression levels of let-7d or miR-205 and grade, angiolymphatic invasion, perineural invasion and alcohol consumption were indicated. No differences were observed in N-stage, tumor localization, gender or patient age. Patients with lower let-7d levels and higher miR-205 levels had significantly better OS (p = 0.0325) than patients with higher let-7d levels and lower miR-205 levels. In the low let-7d level and high miR-205 level group, a lower percentage of more advanced cancers was observed. The analysis of genes related to EMT, cancer stem cells, metastasis, cell cycle, drug response and irradiation response revealed a distinct phenotype of analyzed groups. CONCLUSIONS: The present findings indicated that let-7d down-regulation and miR-205 overexpression create a unique cell phenotype with different behavior compared to cells with upregulated let-7d and down-regulated miR-205. Thus, let-7d and miR-205 are good candidates for new HNSCC biomarkers. |
format | Online Article Text |
id | pubmed-6373017 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-63730172019-02-25 Low let-7d and high miR-205 expression levels positively influence HNSCC patient outcome Kolenda, Tomasz Guglas, Kacper Teresiak, Anna Bliźniak, Renata Lamperska, Katarzyna J Biomed Sci Research INTRODUCTION: Head and neck squamous carcinoma (HNSCC) is one of the most invasive types of cancer with high mortality. A previous study has indicated that low levels of let-7d and miR-205 in HNSCC patients are correlated with poor survival. Let-7d and miR-205 are tumor suppressors and regulators of epithelial-to-mesenchymal transition (EMT). However, it is unclear if let-7d and miR-205 together influence cancer cells. AIM: To determine if let-7d and miR-205 expression levels influence HNSCC patient outcome. METHODS: The TCGA expression data for let-7d, miR-205 and their targets as well as clinical data were downloaded from cBioPortal and starBase v2.0 for 307 patients. The expression levels of let-7d and miR-205 were verified according to clinicopathological parameters. The let-7d and miR-205 high- and low-expression groups as well as disease-free survival (DFS), overall survival (OS) and expression levels of genes related to EMT, cancer stem cells, metastasis, cell cycle, drug response and irradiation response were investigated. RESULTS: Let-7d and miR-205 were frequently upregulated in HNSCC compared to normal samples, and ROC analysis showed high discrimination ability for let-7d and miR-205 (area 0.7369 and 0.7739, respectively; p < 0.0001). Differences between expression levels of let-7d or miR-205 and grade, angiolymphatic invasion, perineural invasion and alcohol consumption were indicated. No differences were observed in N-stage, tumor localization, gender or patient age. Patients with lower let-7d levels and higher miR-205 levels had significantly better OS (p = 0.0325) than patients with higher let-7d levels and lower miR-205 levels. In the low let-7d level and high miR-205 level group, a lower percentage of more advanced cancers was observed. The analysis of genes related to EMT, cancer stem cells, metastasis, cell cycle, drug response and irradiation response revealed a distinct phenotype of analyzed groups. CONCLUSIONS: The present findings indicated that let-7d down-regulation and miR-205 overexpression create a unique cell phenotype with different behavior compared to cells with upregulated let-7d and down-regulated miR-205. Thus, let-7d and miR-205 are good candidates for new HNSCC biomarkers. BioMed Central 2019-02-13 /pmc/articles/PMC6373017/ /pubmed/30755200 http://dx.doi.org/10.1186/s12929-019-0511-3 Text en © The Author(s). 2019 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Research Kolenda, Tomasz Guglas, Kacper Teresiak, Anna Bliźniak, Renata Lamperska, Katarzyna Low let-7d and high miR-205 expression levels positively influence HNSCC patient outcome |
title | Low let-7d and high miR-205 expression levels positively influence HNSCC patient outcome |
title_full | Low let-7d and high miR-205 expression levels positively influence HNSCC patient outcome |
title_fullStr | Low let-7d and high miR-205 expression levels positively influence HNSCC patient outcome |
title_full_unstemmed | Low let-7d and high miR-205 expression levels positively influence HNSCC patient outcome |
title_short | Low let-7d and high miR-205 expression levels positively influence HNSCC patient outcome |
title_sort | low let-7d and high mir-205 expression levels positively influence hnscc patient outcome |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6373017/ https://www.ncbi.nlm.nih.gov/pubmed/30755200 http://dx.doi.org/10.1186/s12929-019-0511-3 |
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