Cargando…

Development and application of high-resolution melting analysis for the classification of infectious laryngotracheitis virus strains and detection of recombinant progeny

Live attenuated vaccines against infectious laryngotracheitis virus (ILTV) are widely used in the poultry industry to control disease and help prevent economic losses. Molecular epidemiological studies of currently circulating strains of ILTV within poultry flocks in Australia have demonstrated the...

Descripción completa

Detalles Bibliográficos
Autores principales: Fakhri, Omid, Hartley, Carol A., Devlin, Joanne M., Browning, Glenn F., Noormohammadi, Amir H., Lee, Sang-Won
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Vienna 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6373279/
https://www.ncbi.nlm.nih.gov/pubmed/30421085
http://dx.doi.org/10.1007/s00705-018-4086-1
_version_ 1783394952742436864
author Fakhri, Omid
Hartley, Carol A.
Devlin, Joanne M.
Browning, Glenn F.
Noormohammadi, Amir H.
Lee, Sang-Won
author_facet Fakhri, Omid
Hartley, Carol A.
Devlin, Joanne M.
Browning, Glenn F.
Noormohammadi, Amir H.
Lee, Sang-Won
author_sort Fakhri, Omid
collection PubMed
description Live attenuated vaccines against infectious laryngotracheitis virus (ILTV) are widely used in the poultry industry to control disease and help prevent economic losses. Molecular epidemiological studies of currently circulating strains of ILTV within poultry flocks in Australia have demonstrated the presence of highly virulent viruses generated by genomic recombination events between vaccine strains. In this study, high-resolution melting (HRM) analysis was used to develop a tool to classify ILTV isolates and to investigate ILTV recombination. The assay was applied to plaque-purified progeny viruses generated after co-infection of chicken embryo kidney (CEK) monolayers with the A20 and Serva ILT vaccine strains and also to viruses isolated from field samples. The results showed that the HRM analysis is a suitable tool for the classification of ILTV isolates and can be used to detect recombination between ILTV vaccine strains in vitro. This method can be used to classify a broad range of ILTV strains to facilitate the classification and genotyping of ILTV and help to further understand recombination in these viruses. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1007/s00705-018-4086-1) contains supplementary material, which is available to authorized users.
format Online
Article
Text
id pubmed-6373279
institution National Center for Biotechnology Information
language English
publishDate 2018
publisher Springer Vienna
record_format MEDLINE/PubMed
spelling pubmed-63732792019-03-01 Development and application of high-resolution melting analysis for the classification of infectious laryngotracheitis virus strains and detection of recombinant progeny Fakhri, Omid Hartley, Carol A. Devlin, Joanne M. Browning, Glenn F. Noormohammadi, Amir H. Lee, Sang-Won Arch Virol Original Article Live attenuated vaccines against infectious laryngotracheitis virus (ILTV) are widely used in the poultry industry to control disease and help prevent economic losses. Molecular epidemiological studies of currently circulating strains of ILTV within poultry flocks in Australia have demonstrated the presence of highly virulent viruses generated by genomic recombination events between vaccine strains. In this study, high-resolution melting (HRM) analysis was used to develop a tool to classify ILTV isolates and to investigate ILTV recombination. The assay was applied to plaque-purified progeny viruses generated after co-infection of chicken embryo kidney (CEK) monolayers with the A20 and Serva ILT vaccine strains and also to viruses isolated from field samples. The results showed that the HRM analysis is a suitable tool for the classification of ILTV isolates and can be used to detect recombination between ILTV vaccine strains in vitro. This method can be used to classify a broad range of ILTV strains to facilitate the classification and genotyping of ILTV and help to further understand recombination in these viruses. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1007/s00705-018-4086-1) contains supplementary material, which is available to authorized users. Springer Vienna 2018-11-12 2019 /pmc/articles/PMC6373279/ /pubmed/30421085 http://dx.doi.org/10.1007/s00705-018-4086-1 Text en © The Author(s) 2018 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.
spellingShingle Original Article
Fakhri, Omid
Hartley, Carol A.
Devlin, Joanne M.
Browning, Glenn F.
Noormohammadi, Amir H.
Lee, Sang-Won
Development and application of high-resolution melting analysis for the classification of infectious laryngotracheitis virus strains and detection of recombinant progeny
title Development and application of high-resolution melting analysis for the classification of infectious laryngotracheitis virus strains and detection of recombinant progeny
title_full Development and application of high-resolution melting analysis for the classification of infectious laryngotracheitis virus strains and detection of recombinant progeny
title_fullStr Development and application of high-resolution melting analysis for the classification of infectious laryngotracheitis virus strains and detection of recombinant progeny
title_full_unstemmed Development and application of high-resolution melting analysis for the classification of infectious laryngotracheitis virus strains and detection of recombinant progeny
title_short Development and application of high-resolution melting analysis for the classification of infectious laryngotracheitis virus strains and detection of recombinant progeny
title_sort development and application of high-resolution melting analysis for the classification of infectious laryngotracheitis virus strains and detection of recombinant progeny
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6373279/
https://www.ncbi.nlm.nih.gov/pubmed/30421085
http://dx.doi.org/10.1007/s00705-018-4086-1
work_keys_str_mv AT fakhriomid developmentandapplicationofhighresolutionmeltinganalysisfortheclassificationofinfectiouslaryngotracheitisvirusstrainsanddetectionofrecombinantprogeny
AT hartleycarola developmentandapplicationofhighresolutionmeltinganalysisfortheclassificationofinfectiouslaryngotracheitisvirusstrainsanddetectionofrecombinantprogeny
AT devlinjoannem developmentandapplicationofhighresolutionmeltinganalysisfortheclassificationofinfectiouslaryngotracheitisvirusstrainsanddetectionofrecombinantprogeny
AT browningglennf developmentandapplicationofhighresolutionmeltinganalysisfortheclassificationofinfectiouslaryngotracheitisvirusstrainsanddetectionofrecombinantprogeny
AT noormohammadiamirh developmentandapplicationofhighresolutionmeltinganalysisfortheclassificationofinfectiouslaryngotracheitisvirusstrainsanddetectionofrecombinantprogeny
AT leesangwon developmentandapplicationofhighresolutionmeltinganalysisfortheclassificationofinfectiouslaryngotracheitisvirusstrainsanddetectionofrecombinantprogeny