Cargando…

Simplified Techniques for Killing the Carcinogenic, Opisthorchis Viverrini Metacercariae in Cyprinid Fish

Consumption of fluke-free fish is the most important factor in controlling Opisthorchis viverrini (OV) infection in endemic areas such as northeast Thailand and thereby reducing the risk of cholangiocarcinoma. Cooking fish is the best way to avoid infection; however, the cultural practice of eating...

Descripción completa

Detalles Bibliográficos
Autores principales: Sripan, Panupan, Boonmars, Thidarut, Songsri, Jiraporn, Aukkanimart, Ratchadawan, Sriraj, Pranee, Rattanasuwan, Panaratana, Boueroy, Parichart, Suwannatrai, Apiporn, Aunpromma, Surasit, Khuntikeo, Narong, Loilome, Watcharin, Namwat, Nisana, Yongvanit, Puangrat, Wai, Aung Phyo, Khueangchaingkhwang, Sukhonthip, Zhilang, Wu, Pumhirunroj, Benjamabhorn, Artchayasawat, Atchara, Boonjaraspinyo, Sirintip
Formato: Online Artículo Texto
Lenguaje:English
Publicado: West Asia Organization for Cancer Prevention 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6373821/
https://www.ncbi.nlm.nih.gov/pubmed/28669159
http://dx.doi.org/10.22034/APJCP.2017.18.6.1507
_version_ 1783395051580162048
author Sripan, Panupan
Boonmars, Thidarut
Songsri, Jiraporn
Aukkanimart, Ratchadawan
Sriraj, Pranee
Rattanasuwan, Panaratana
Boueroy, Parichart
Suwannatrai, Apiporn
Aunpromma, Surasit
Khuntikeo, Narong
Loilome, Watcharin
Namwat, Nisana
Yongvanit, Puangrat
Wai, Aung Phyo
Khueangchaingkhwang, Sukhonthip
Zhilang, Wu
Pumhirunroj, Benjamabhorn
Artchayasawat, Atchara
Boonjaraspinyo, Sirintip
author_facet Sripan, Panupan
Boonmars, Thidarut
Songsri, Jiraporn
Aukkanimart, Ratchadawan
Sriraj, Pranee
Rattanasuwan, Panaratana
Boueroy, Parichart
Suwannatrai, Apiporn
Aunpromma, Surasit
Khuntikeo, Narong
Loilome, Watcharin
Namwat, Nisana
Yongvanit, Puangrat
Wai, Aung Phyo
Khueangchaingkhwang, Sukhonthip
Zhilang, Wu
Pumhirunroj, Benjamabhorn
Artchayasawat, Atchara
Boonjaraspinyo, Sirintip
author_sort Sripan, Panupan
collection PubMed
description Consumption of fluke-free fish is the most important factor in controlling Opisthorchis viverrini (OV) infection in endemic areas such as northeast Thailand and thereby reducing the risk of cholangiocarcinoma. Cooking fish is the best way to avoid infection; however, the cultural practice of eating raw or fermented fish is difficult to change. We investigated the food preparation process, using freezing, heating and fermentation to kill OV metacercariae in fish. Uncooked cyprinid fish infected with OV were divided into three groups: refrigerated at 4 °C for 24, 48 or 72 h (control group); frozen at -20 °C for 24, 48 or 72 h; or heated by microwaving (at 400 or 800 W) or boiling at 90 °C for 1, 5 or 10 min. Moreover, pickled (fermented) fish were divided into two groups: refrigerated at 4 °C (control) or frozen at -20 °C for 24 or 48 h. The infectivity of recovered metacercariae was confirmed by infecting hamsters with OV and then evaluating the recovery of adult worms after 1 month. We found that a heating process, by boiling or microwaving at 400 or 800 W for at least 5 min, could kill OV metacercariae, and freezing pickled fish at -20 °C for 48 h could kill OV metacercariae in all sizes of fish. The present study found that heating and freezing processes, as well as the fermentation process under optimal conditions, could kill OV metacercariae in a timely manner. This knowledge is valuable for implementation in endemic areas to control OV infection and cholangiocarcinoma.
format Online
Article
Text
id pubmed-6373821
institution National Center for Biotechnology Information
language English
publishDate 2017
publisher West Asia Organization for Cancer Prevention
record_format MEDLINE/PubMed
spelling pubmed-63738212019-03-19 Simplified Techniques for Killing the Carcinogenic, Opisthorchis Viverrini Metacercariae in Cyprinid Fish Sripan, Panupan Boonmars, Thidarut Songsri, Jiraporn Aukkanimart, Ratchadawan Sriraj, Pranee Rattanasuwan, Panaratana Boueroy, Parichart Suwannatrai, Apiporn Aunpromma, Surasit Khuntikeo, Narong Loilome, Watcharin Namwat, Nisana Yongvanit, Puangrat Wai, Aung Phyo Khueangchaingkhwang, Sukhonthip Zhilang, Wu Pumhirunroj, Benjamabhorn Artchayasawat, Atchara Boonjaraspinyo, Sirintip Asian Pac J Cancer Prev Research Article Consumption of fluke-free fish is the most important factor in controlling Opisthorchis viverrini (OV) infection in endemic areas such as northeast Thailand and thereby reducing the risk of cholangiocarcinoma. Cooking fish is the best way to avoid infection; however, the cultural practice of eating raw or fermented fish is difficult to change. We investigated the food preparation process, using freezing, heating and fermentation to kill OV metacercariae in fish. Uncooked cyprinid fish infected with OV were divided into three groups: refrigerated at 4 °C for 24, 48 or 72 h (control group); frozen at -20 °C for 24, 48 or 72 h; or heated by microwaving (at 400 or 800 W) or boiling at 90 °C for 1, 5 or 10 min. Moreover, pickled (fermented) fish were divided into two groups: refrigerated at 4 °C (control) or frozen at -20 °C for 24 or 48 h. The infectivity of recovered metacercariae was confirmed by infecting hamsters with OV and then evaluating the recovery of adult worms after 1 month. We found that a heating process, by boiling or microwaving at 400 or 800 W for at least 5 min, could kill OV metacercariae, and freezing pickled fish at -20 °C for 48 h could kill OV metacercariae in all sizes of fish. The present study found that heating and freezing processes, as well as the fermentation process under optimal conditions, could kill OV metacercariae in a timely manner. This knowledge is valuable for implementation in endemic areas to control OV infection and cholangiocarcinoma. West Asia Organization for Cancer Prevention 2017 /pmc/articles/PMC6373821/ /pubmed/28669159 http://dx.doi.org/10.22034/APJCP.2017.18.6.1507 Text en Copyright: © Asian Pacific Journal of Cancer Prevention http://creativecommons.org/licenses/BY-SA/4.0 This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License
spellingShingle Research Article
Sripan, Panupan
Boonmars, Thidarut
Songsri, Jiraporn
Aukkanimart, Ratchadawan
Sriraj, Pranee
Rattanasuwan, Panaratana
Boueroy, Parichart
Suwannatrai, Apiporn
Aunpromma, Surasit
Khuntikeo, Narong
Loilome, Watcharin
Namwat, Nisana
Yongvanit, Puangrat
Wai, Aung Phyo
Khueangchaingkhwang, Sukhonthip
Zhilang, Wu
Pumhirunroj, Benjamabhorn
Artchayasawat, Atchara
Boonjaraspinyo, Sirintip
Simplified Techniques for Killing the Carcinogenic, Opisthorchis Viverrini Metacercariae in Cyprinid Fish
title Simplified Techniques for Killing the Carcinogenic, Opisthorchis Viverrini Metacercariae in Cyprinid Fish
title_full Simplified Techniques for Killing the Carcinogenic, Opisthorchis Viverrini Metacercariae in Cyprinid Fish
title_fullStr Simplified Techniques for Killing the Carcinogenic, Opisthorchis Viverrini Metacercariae in Cyprinid Fish
title_full_unstemmed Simplified Techniques for Killing the Carcinogenic, Opisthorchis Viverrini Metacercariae in Cyprinid Fish
title_short Simplified Techniques for Killing the Carcinogenic, Opisthorchis Viverrini Metacercariae in Cyprinid Fish
title_sort simplified techniques for killing the carcinogenic, opisthorchis viverrini metacercariae in cyprinid fish
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6373821/
https://www.ncbi.nlm.nih.gov/pubmed/28669159
http://dx.doi.org/10.22034/APJCP.2017.18.6.1507
work_keys_str_mv AT sripanpanupan simplifiedtechniquesforkillingthecarcinogenicopisthorchisviverrinimetacercariaeincyprinidfish
AT boonmarsthidarut simplifiedtechniquesforkillingthecarcinogenicopisthorchisviverrinimetacercariaeincyprinidfish
AT songsrijiraporn simplifiedtechniquesforkillingthecarcinogenicopisthorchisviverrinimetacercariaeincyprinidfish
AT aukkanimartratchadawan simplifiedtechniquesforkillingthecarcinogenicopisthorchisviverrinimetacercariaeincyprinidfish
AT srirajpranee simplifiedtechniquesforkillingthecarcinogenicopisthorchisviverrinimetacercariaeincyprinidfish
AT rattanasuwanpanaratana simplifiedtechniquesforkillingthecarcinogenicopisthorchisviverrinimetacercariaeincyprinidfish
AT boueroyparichart simplifiedtechniquesforkillingthecarcinogenicopisthorchisviverrinimetacercariaeincyprinidfish
AT suwannatraiapiporn simplifiedtechniquesforkillingthecarcinogenicopisthorchisviverrinimetacercariaeincyprinidfish
AT aunprommasurasit simplifiedtechniquesforkillingthecarcinogenicopisthorchisviverrinimetacercariaeincyprinidfish
AT khuntikeonarong simplifiedtechniquesforkillingthecarcinogenicopisthorchisviverrinimetacercariaeincyprinidfish
AT loilomewatcharin simplifiedtechniquesforkillingthecarcinogenicopisthorchisviverrinimetacercariaeincyprinidfish
AT namwatnisana simplifiedtechniquesforkillingthecarcinogenicopisthorchisviverrinimetacercariaeincyprinidfish
AT yongvanitpuangrat simplifiedtechniquesforkillingthecarcinogenicopisthorchisviverrinimetacercariaeincyprinidfish
AT waiaungphyo simplifiedtechniquesforkillingthecarcinogenicopisthorchisviverrinimetacercariaeincyprinidfish
AT khueangchaingkhwangsukhonthip simplifiedtechniquesforkillingthecarcinogenicopisthorchisviverrinimetacercariaeincyprinidfish
AT zhilangwu simplifiedtechniquesforkillingthecarcinogenicopisthorchisviverrinimetacercariaeincyprinidfish
AT pumhirunrojbenjamabhorn simplifiedtechniquesforkillingthecarcinogenicopisthorchisviverrinimetacercariaeincyprinidfish
AT artchayasawatatchara simplifiedtechniquesforkillingthecarcinogenicopisthorchisviverrinimetacercariaeincyprinidfish
AT boonjaraspinyosirintip simplifiedtechniquesforkillingthecarcinogenicopisthorchisviverrinimetacercariaeincyprinidfish