Cargando…

Intracellular Vesicle Trafficking Genes, RabC-GTP, Are Highly Expressed Under Salinity and Rapid Dehydration but Down-Regulated by Drought in Leaves of Chickpea (Cicer arietinum L.)

Intracellular vesicle trafficking genes, Rab, encoding small GTP binding proteins, have been well studied in medical research, but there is little information concerning these proteins in plants. Some sub-families of the Rab genes have not yet been characterized in plants, such as RabC – otherwise k...

Descripción completa

Detalles Bibliográficos
Autores principales: Khassanova, Gulmira, Kurishbayev, Akhylbek, Jatayev, Satyvaldy, Zhubatkanov, Askar, Zhumalin, Aybek, Turbekova, Arysgul, Amantaev, Bekzak, Lopato, Sergiy, Schramm, Carly, Jenkins, Colin, Soole, Kathleen, Langridge, Peter, Shavrukov, Yuri
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6374294/
https://www.ncbi.nlm.nih.gov/pubmed/30792734
http://dx.doi.org/10.3389/fgene.2019.00040
_version_ 1783395110714605568
author Khassanova, Gulmira
Kurishbayev, Akhylbek
Jatayev, Satyvaldy
Zhubatkanov, Askar
Zhumalin, Aybek
Turbekova, Arysgul
Amantaev, Bekzak
Lopato, Sergiy
Schramm, Carly
Jenkins, Colin
Soole, Kathleen
Langridge, Peter
Shavrukov, Yuri
author_facet Khassanova, Gulmira
Kurishbayev, Akhylbek
Jatayev, Satyvaldy
Zhubatkanov, Askar
Zhumalin, Aybek
Turbekova, Arysgul
Amantaev, Bekzak
Lopato, Sergiy
Schramm, Carly
Jenkins, Colin
Soole, Kathleen
Langridge, Peter
Shavrukov, Yuri
author_sort Khassanova, Gulmira
collection PubMed
description Intracellular vesicle trafficking genes, Rab, encoding small GTP binding proteins, have been well studied in medical research, but there is little information concerning these proteins in plants. Some sub-families of the Rab genes have not yet been characterized in plants, such as RabC – otherwise known as Rab18 in yeast and animals. Our study aimed to identify all CaRab gene sequences in chickpea (Cicer arietinum L.) using bioinformatics approaches, with a particular focus on the CaRabC gene sub-family since it featured in an SNP database. Five isoforms of the CaRabC gene were identified and studied: CaRabC-1a, -1b, -1c, -2a and -2a(∗). Six accessions of both Desi and Kabuli ecotypes, selected from field trials, were tested for tolerance to abiotic stresses, including salinity, drought and rapid dehydration and compared to plant growth under control conditions. Expression analysis of total and individual CaRabC isoforms in leaves of control plants revealed a very high level of expression, with the greatest contribution made by CaRabC-1c. Salinity stress (150 mM NaCl, 12 days in soil) caused a 2-3-fold increased expression of total CaRabC compared to controls, with the highest expression in isoforms CaRabC-1c, -2a(∗) and -1a. Significantly decreased expression of all five isoforms of CaRabC was observed under drought (12 days withheld water) compared to controls. In contrast, both total CaRabC and the CaRabC-1a isoform showed very high expression (up-to eight-fold) in detached leaves over 6 h of dehydration. The results suggest that the CaRabC gene is involved in plant growth and response to abiotic stresses. It was highly expressed in leaves of non-stressed plants and was down-regulated after drought, but salinity and rapid dehydration caused up-regulation to high and very high levels, respectively. The isoforms of CaRabC were differentially expressed, with the highest levels recorded for CaRabC-1c in controls and under salinity stress, and for CaRabC-1a – in rapidly dehydrated leaves. Genotypic variation in CaRabC-1a, comprising eleven SNPs, was found through sequencing of the local chickpea cultivar Yubileiny and germplasm ICC7255 in comparison to the two fully sequenced reference accessions, ICC4958 and Frontier. Amplifluor-like markers based on one of the identified SNPs in CaRabC-1a were designed and successfully used for genotyping chickpea germplasm.
format Online
Article
Text
id pubmed-6374294
institution National Center for Biotechnology Information
language English
publishDate 2019
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-63742942019-02-21 Intracellular Vesicle Trafficking Genes, RabC-GTP, Are Highly Expressed Under Salinity and Rapid Dehydration but Down-Regulated by Drought in Leaves of Chickpea (Cicer arietinum L.) Khassanova, Gulmira Kurishbayev, Akhylbek Jatayev, Satyvaldy Zhubatkanov, Askar Zhumalin, Aybek Turbekova, Arysgul Amantaev, Bekzak Lopato, Sergiy Schramm, Carly Jenkins, Colin Soole, Kathleen Langridge, Peter Shavrukov, Yuri Front Genet Genetics Intracellular vesicle trafficking genes, Rab, encoding small GTP binding proteins, have been well studied in medical research, but there is little information concerning these proteins in plants. Some sub-families of the Rab genes have not yet been characterized in plants, such as RabC – otherwise known as Rab18 in yeast and animals. Our study aimed to identify all CaRab gene sequences in chickpea (Cicer arietinum L.) using bioinformatics approaches, with a particular focus on the CaRabC gene sub-family since it featured in an SNP database. Five isoforms of the CaRabC gene were identified and studied: CaRabC-1a, -1b, -1c, -2a and -2a(∗). Six accessions of both Desi and Kabuli ecotypes, selected from field trials, were tested for tolerance to abiotic stresses, including salinity, drought and rapid dehydration and compared to plant growth under control conditions. Expression analysis of total and individual CaRabC isoforms in leaves of control plants revealed a very high level of expression, with the greatest contribution made by CaRabC-1c. Salinity stress (150 mM NaCl, 12 days in soil) caused a 2-3-fold increased expression of total CaRabC compared to controls, with the highest expression in isoforms CaRabC-1c, -2a(∗) and -1a. Significantly decreased expression of all five isoforms of CaRabC was observed under drought (12 days withheld water) compared to controls. In contrast, both total CaRabC and the CaRabC-1a isoform showed very high expression (up-to eight-fold) in detached leaves over 6 h of dehydration. The results suggest that the CaRabC gene is involved in plant growth and response to abiotic stresses. It was highly expressed in leaves of non-stressed plants and was down-regulated after drought, but salinity and rapid dehydration caused up-regulation to high and very high levels, respectively. The isoforms of CaRabC were differentially expressed, with the highest levels recorded for CaRabC-1c in controls and under salinity stress, and for CaRabC-1a – in rapidly dehydrated leaves. Genotypic variation in CaRabC-1a, comprising eleven SNPs, was found through sequencing of the local chickpea cultivar Yubileiny and germplasm ICC7255 in comparison to the two fully sequenced reference accessions, ICC4958 and Frontier. Amplifluor-like markers based on one of the identified SNPs in CaRabC-1a were designed and successfully used for genotyping chickpea germplasm. Frontiers Media S.A. 2019-02-07 /pmc/articles/PMC6374294/ /pubmed/30792734 http://dx.doi.org/10.3389/fgene.2019.00040 Text en Copyright © 2019 Khassanova, Kurishbayev, Jatayev, Zhubatkanov, Zhumalin, Turbekova, Amantaev, Lopato, Schramm, Jenkins, Soole, Langridge and Shavrukov. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Genetics
Khassanova, Gulmira
Kurishbayev, Akhylbek
Jatayev, Satyvaldy
Zhubatkanov, Askar
Zhumalin, Aybek
Turbekova, Arysgul
Amantaev, Bekzak
Lopato, Sergiy
Schramm, Carly
Jenkins, Colin
Soole, Kathleen
Langridge, Peter
Shavrukov, Yuri
Intracellular Vesicle Trafficking Genes, RabC-GTP, Are Highly Expressed Under Salinity and Rapid Dehydration but Down-Regulated by Drought in Leaves of Chickpea (Cicer arietinum L.)
title Intracellular Vesicle Trafficking Genes, RabC-GTP, Are Highly Expressed Under Salinity and Rapid Dehydration but Down-Regulated by Drought in Leaves of Chickpea (Cicer arietinum L.)
title_full Intracellular Vesicle Trafficking Genes, RabC-GTP, Are Highly Expressed Under Salinity and Rapid Dehydration but Down-Regulated by Drought in Leaves of Chickpea (Cicer arietinum L.)
title_fullStr Intracellular Vesicle Trafficking Genes, RabC-GTP, Are Highly Expressed Under Salinity and Rapid Dehydration but Down-Regulated by Drought in Leaves of Chickpea (Cicer arietinum L.)
title_full_unstemmed Intracellular Vesicle Trafficking Genes, RabC-GTP, Are Highly Expressed Under Salinity and Rapid Dehydration but Down-Regulated by Drought in Leaves of Chickpea (Cicer arietinum L.)
title_short Intracellular Vesicle Trafficking Genes, RabC-GTP, Are Highly Expressed Under Salinity and Rapid Dehydration but Down-Regulated by Drought in Leaves of Chickpea (Cicer arietinum L.)
title_sort intracellular vesicle trafficking genes, rabc-gtp, are highly expressed under salinity and rapid dehydration but down-regulated by drought in leaves of chickpea (cicer arietinum l.)
topic Genetics
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6374294/
https://www.ncbi.nlm.nih.gov/pubmed/30792734
http://dx.doi.org/10.3389/fgene.2019.00040
work_keys_str_mv AT khassanovagulmira intracellularvesicletraffickinggenesrabcgtparehighlyexpressedundersalinityandrapiddehydrationbutdownregulatedbydroughtinleavesofchickpeacicerarietinuml
AT kurishbayevakhylbek intracellularvesicletraffickinggenesrabcgtparehighlyexpressedundersalinityandrapiddehydrationbutdownregulatedbydroughtinleavesofchickpeacicerarietinuml
AT jatayevsatyvaldy intracellularvesicletraffickinggenesrabcgtparehighlyexpressedundersalinityandrapiddehydrationbutdownregulatedbydroughtinleavesofchickpeacicerarietinuml
AT zhubatkanovaskar intracellularvesicletraffickinggenesrabcgtparehighlyexpressedundersalinityandrapiddehydrationbutdownregulatedbydroughtinleavesofchickpeacicerarietinuml
AT zhumalinaybek intracellularvesicletraffickinggenesrabcgtparehighlyexpressedundersalinityandrapiddehydrationbutdownregulatedbydroughtinleavesofchickpeacicerarietinuml
AT turbekovaarysgul intracellularvesicletraffickinggenesrabcgtparehighlyexpressedundersalinityandrapiddehydrationbutdownregulatedbydroughtinleavesofchickpeacicerarietinuml
AT amantaevbekzak intracellularvesicletraffickinggenesrabcgtparehighlyexpressedundersalinityandrapiddehydrationbutdownregulatedbydroughtinleavesofchickpeacicerarietinuml
AT lopatosergiy intracellularvesicletraffickinggenesrabcgtparehighlyexpressedundersalinityandrapiddehydrationbutdownregulatedbydroughtinleavesofchickpeacicerarietinuml
AT schrammcarly intracellularvesicletraffickinggenesrabcgtparehighlyexpressedundersalinityandrapiddehydrationbutdownregulatedbydroughtinleavesofchickpeacicerarietinuml
AT jenkinscolin intracellularvesicletraffickinggenesrabcgtparehighlyexpressedundersalinityandrapiddehydrationbutdownregulatedbydroughtinleavesofchickpeacicerarietinuml
AT soolekathleen intracellularvesicletraffickinggenesrabcgtparehighlyexpressedundersalinityandrapiddehydrationbutdownregulatedbydroughtinleavesofchickpeacicerarietinuml
AT langridgepeter intracellularvesicletraffickinggenesrabcgtparehighlyexpressedundersalinityandrapiddehydrationbutdownregulatedbydroughtinleavesofchickpeacicerarietinuml
AT shavrukovyuri intracellularvesicletraffickinggenesrabcgtparehighlyexpressedundersalinityandrapiddehydrationbutdownregulatedbydroughtinleavesofchickpeacicerarietinuml