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Synthesis of salt-stable fluorescent nanoparticles (quantum dots) by polyextremophile halophilic bacteria

Here we report the biological synthesis of CdS fluorescent nanoparticles (Quantum Dots, QDs) by polyextremophile halophilic bacteria isolated from Atacama Salt Flat (Chile), Uyuni Salt Flat (Bolivia) and the Dead Sea (Israel). In particular, a Halobacillus sp. DS2, a strain presenting high resistanc...

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Autores principales: Bruna, N., Collao, B., Tello, A., Caravantes, P., Díaz-Silva, N., Monrás, J. P., Órdenes-Aenishanslins, N., Flores, M., Espinoza-Gonzalez, R., Bravo, D., Pérez-Donoso, J. M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6374371/
https://www.ncbi.nlm.nih.gov/pubmed/30760793
http://dx.doi.org/10.1038/s41598-018-38330-8
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author Bruna, N.
Collao, B.
Tello, A.
Caravantes, P.
Díaz-Silva, N.
Monrás, J. P.
Órdenes-Aenishanslins, N.
Flores, M.
Espinoza-Gonzalez, R.
Bravo, D.
Pérez-Donoso, J. M.
author_facet Bruna, N.
Collao, B.
Tello, A.
Caravantes, P.
Díaz-Silva, N.
Monrás, J. P.
Órdenes-Aenishanslins, N.
Flores, M.
Espinoza-Gonzalez, R.
Bravo, D.
Pérez-Donoso, J. M.
author_sort Bruna, N.
collection PubMed
description Here we report the biological synthesis of CdS fluorescent nanoparticles (Quantum Dots, QDs) by polyextremophile halophilic bacteria isolated from Atacama Salt Flat (Chile), Uyuni Salt Flat (Bolivia) and the Dead Sea (Israel). In particular, a Halobacillus sp. DS2, a strain presenting high resistance to NaCl (3–22%), acidic pH (1–4) and cadmium (CdCl(2) MIC: 1,375 mM) was used for QDs biosynthesis studies. Halobacillus sp. synthesize CdS QDs in presence of high NaCl concentrations in a process related with their capacity to generate S(2−) in these conditions. Biosynthesized QDs were purified, characterized and their stability at different NaCl concentrations determined. Hexagonal nanoparticles with highly defined structures (hexagonal phase), monodisperse size distribution (2–5 nm) and composed by CdS, NaCl and cysteine were determined by TEM, EDX, HRXPS and FTIR. In addition, QDs biosynthesized by Halobacillus sp. DS2 displayed increased tolerance to NaCl when compared to QDs produced chemically or biosynthesized by non-halophilic bacteria. This is the first report of biological synthesis of salt-stable QDs and confirms the potential of using extremophile microorganisms to produce novel nanoparticles. Obtained results constitute a new alternative to improve QDs properties, and as consequence, to increase their industrial and biomedical applications.
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spelling pubmed-63743712019-02-19 Synthesis of salt-stable fluorescent nanoparticles (quantum dots) by polyextremophile halophilic bacteria Bruna, N. Collao, B. Tello, A. Caravantes, P. Díaz-Silva, N. Monrás, J. P. Órdenes-Aenishanslins, N. Flores, M. Espinoza-Gonzalez, R. Bravo, D. Pérez-Donoso, J. M. Sci Rep Article Here we report the biological synthesis of CdS fluorescent nanoparticles (Quantum Dots, QDs) by polyextremophile halophilic bacteria isolated from Atacama Salt Flat (Chile), Uyuni Salt Flat (Bolivia) and the Dead Sea (Israel). In particular, a Halobacillus sp. DS2, a strain presenting high resistance to NaCl (3–22%), acidic pH (1–4) and cadmium (CdCl(2) MIC: 1,375 mM) was used for QDs biosynthesis studies. Halobacillus sp. synthesize CdS QDs in presence of high NaCl concentrations in a process related with their capacity to generate S(2−) in these conditions. Biosynthesized QDs were purified, characterized and their stability at different NaCl concentrations determined. Hexagonal nanoparticles with highly defined structures (hexagonal phase), monodisperse size distribution (2–5 nm) and composed by CdS, NaCl and cysteine were determined by TEM, EDX, HRXPS and FTIR. In addition, QDs biosynthesized by Halobacillus sp. DS2 displayed increased tolerance to NaCl when compared to QDs produced chemically or biosynthesized by non-halophilic bacteria. This is the first report of biological synthesis of salt-stable QDs and confirms the potential of using extremophile microorganisms to produce novel nanoparticles. Obtained results constitute a new alternative to improve QDs properties, and as consequence, to increase their industrial and biomedical applications. Nature Publishing Group UK 2019-02-13 /pmc/articles/PMC6374371/ /pubmed/30760793 http://dx.doi.org/10.1038/s41598-018-38330-8 Text en © The Author(s) 2019 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Bruna, N.
Collao, B.
Tello, A.
Caravantes, P.
Díaz-Silva, N.
Monrás, J. P.
Órdenes-Aenishanslins, N.
Flores, M.
Espinoza-Gonzalez, R.
Bravo, D.
Pérez-Donoso, J. M.
Synthesis of salt-stable fluorescent nanoparticles (quantum dots) by polyextremophile halophilic bacteria
title Synthesis of salt-stable fluorescent nanoparticles (quantum dots) by polyextremophile halophilic bacteria
title_full Synthesis of salt-stable fluorescent nanoparticles (quantum dots) by polyextremophile halophilic bacteria
title_fullStr Synthesis of salt-stable fluorescent nanoparticles (quantum dots) by polyextremophile halophilic bacteria
title_full_unstemmed Synthesis of salt-stable fluorescent nanoparticles (quantum dots) by polyextremophile halophilic bacteria
title_short Synthesis of salt-stable fluorescent nanoparticles (quantum dots) by polyextremophile halophilic bacteria
title_sort synthesis of salt-stable fluorescent nanoparticles (quantum dots) by polyextremophile halophilic bacteria
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6374371/
https://www.ncbi.nlm.nih.gov/pubmed/30760793
http://dx.doi.org/10.1038/s41598-018-38330-8
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