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Characteristics of peripheral immune cell subsets in patients with carotid atherosclerosis undergoing carotid endarterectomy

INTRODUCTION: The role of circulating immune cells in the pathophysiology of cerebrovascular accidents is currently under debate. The aim of this study was to characterize peripheral immune cell subsets in patients undergoing carotid endarterectomy (CEA). MATERIAL AND METHODS: We conducted a prospec...

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Autores principales: Kotfis, Katarzyna, Biernawska, Jowita, Zegan-Barańska, Małgorzata, Gutowski, Piotr, Żukowski, Maciej
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Termedia Publishing House 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6374565/
https://www.ncbi.nlm.nih.gov/pubmed/30775603
http://dx.doi.org/10.5114/amsad.2018.79537
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author Kotfis, Katarzyna
Biernawska, Jowita
Zegan-Barańska, Małgorzata
Gutowski, Piotr
Żukowski, Maciej
author_facet Kotfis, Katarzyna
Biernawska, Jowita
Zegan-Barańska, Małgorzata
Gutowski, Piotr
Żukowski, Maciej
author_sort Kotfis, Katarzyna
collection PubMed
description INTRODUCTION: The role of circulating immune cells in the pathophysiology of cerebrovascular accidents is currently under debate. The aim of this study was to characterize peripheral immune cell subsets in patients undergoing carotid endarterectomy (CEA). MATERIAL AND METHODS: We conducted a prospective observational study in a group of 124 patients with significant carotid stenosis undergoing carotid endarterectomy, both symptomatic and asymptomatic. We compared the percentages of circulating immune cells: B lymphocytes, T lymphocytes, T helper, cytotoxic T, CD4+/CD8+ ratio, T regulatory, monocytes and NK cells before CEA and 6 h after the procedure. RESULTS: Total lymphocyte count and cytotoxic T lymphocyte count decreased 6 h after CEA in both subgroups. The NK cell level decrease was statistically significant only in the symptomatic subgroup (19.41 ±9.30 before CEA and 16.52 ±9.37 after CEA; p = 0.0044), but not in the asymptomatic subgroup (17.88 ±9.14 before CEA and 15.91 ±9.51 after CEA; p = 0.0886). The T lymphocyte level showed a statistically significant increase only in symptomatic patients (69.74 ±10.16 before CEA vs. 71.45 ±9.77 after CEA; p = 0.0462), and not in the asymptomatic subgroup (70.08 ±11.19 prior to CEA and 70.21 ±12.35; p = 0.9048). B lymphocyte, helper T lymphocyte and regulatory T (Treg) lymphocyte (CD4+/CD25+) levels showed a significant increase after CEA. CONCLUSIONS: This is the first study to compare circulating immune cells in patients undergoing carotid endarterectomy. Only the symptomatic subgroup experienced a significant decrease in the NK cell level and an increase in the T lymphocyte count after CEA. This study enriches our understanding of immune cell kinetics during carotid endarterectomy.
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spelling pubmed-63745652019-02-15 Characteristics of peripheral immune cell subsets in patients with carotid atherosclerosis undergoing carotid endarterectomy Kotfis, Katarzyna Biernawska, Jowita Zegan-Barańska, Małgorzata Gutowski, Piotr Żukowski, Maciej Arch Med Sci Atheroscler Dis Clinical Research INTRODUCTION: The role of circulating immune cells in the pathophysiology of cerebrovascular accidents is currently under debate. The aim of this study was to characterize peripheral immune cell subsets in patients undergoing carotid endarterectomy (CEA). MATERIAL AND METHODS: We conducted a prospective observational study in a group of 124 patients with significant carotid stenosis undergoing carotid endarterectomy, both symptomatic and asymptomatic. We compared the percentages of circulating immune cells: B lymphocytes, T lymphocytes, T helper, cytotoxic T, CD4+/CD8+ ratio, T regulatory, monocytes and NK cells before CEA and 6 h after the procedure. RESULTS: Total lymphocyte count and cytotoxic T lymphocyte count decreased 6 h after CEA in both subgroups. The NK cell level decrease was statistically significant only in the symptomatic subgroup (19.41 ±9.30 before CEA and 16.52 ±9.37 after CEA; p = 0.0044), but not in the asymptomatic subgroup (17.88 ±9.14 before CEA and 15.91 ±9.51 after CEA; p = 0.0886). The T lymphocyte level showed a statistically significant increase only in symptomatic patients (69.74 ±10.16 before CEA vs. 71.45 ±9.77 after CEA; p = 0.0462), and not in the asymptomatic subgroup (70.08 ±11.19 prior to CEA and 70.21 ±12.35; p = 0.9048). B lymphocyte, helper T lymphocyte and regulatory T (Treg) lymphocyte (CD4+/CD25+) levels showed a significant increase after CEA. CONCLUSIONS: This is the first study to compare circulating immune cells in patients undergoing carotid endarterectomy. Only the symptomatic subgroup experienced a significant decrease in the NK cell level and an increase in the T lymphocyte count after CEA. This study enriches our understanding of immune cell kinetics during carotid endarterectomy. Termedia Publishing House 2018-11-14 /pmc/articles/PMC6374565/ /pubmed/30775603 http://dx.doi.org/10.5114/amsad.2018.79537 Text en Copyright: © 2018 Termedia & Banach http://creativecommons.org/licenses/by-nc-sa/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) License, allowing third parties to copy and redistribute the material in any medium or format and to remix, transform, and build upon the material, provided the original work is properly cited and states its license.
spellingShingle Clinical Research
Kotfis, Katarzyna
Biernawska, Jowita
Zegan-Barańska, Małgorzata
Gutowski, Piotr
Żukowski, Maciej
Characteristics of peripheral immune cell subsets in patients with carotid atherosclerosis undergoing carotid endarterectomy
title Characteristics of peripheral immune cell subsets in patients with carotid atherosclerosis undergoing carotid endarterectomy
title_full Characteristics of peripheral immune cell subsets in patients with carotid atherosclerosis undergoing carotid endarterectomy
title_fullStr Characteristics of peripheral immune cell subsets in patients with carotid atherosclerosis undergoing carotid endarterectomy
title_full_unstemmed Characteristics of peripheral immune cell subsets in patients with carotid atherosclerosis undergoing carotid endarterectomy
title_short Characteristics of peripheral immune cell subsets in patients with carotid atherosclerosis undergoing carotid endarterectomy
title_sort characteristics of peripheral immune cell subsets in patients with carotid atherosclerosis undergoing carotid endarterectomy
topic Clinical Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6374565/
https://www.ncbi.nlm.nih.gov/pubmed/30775603
http://dx.doi.org/10.5114/amsad.2018.79537
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