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Plant-Mediated RNAi for Controlling Apolygus lucorum
The polyphagous mirid bug Apolygus lucorum (Heteroptera: Miridae) is a serious pest of agricultural crops in China, with more than 200 species of host plants including two very important crops, maize and soybean. Currently, prevention and control of A. lucorum rely mainly on chemical pesticides that...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6374644/ https://www.ncbi.nlm.nih.gov/pubmed/30792724 http://dx.doi.org/10.3389/fpls.2019.00064 |
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author | Liu, Fangzhou Yang, Bin Zhang, Aihong Ding, Derong Wang, Guirong |
author_facet | Liu, Fangzhou Yang, Bin Zhang, Aihong Ding, Derong Wang, Guirong |
author_sort | Liu, Fangzhou |
collection | PubMed |
description | The polyphagous mirid bug Apolygus lucorum (Heteroptera: Miridae) is a serious pest of agricultural crops in China, with more than 200 species of host plants including two very important crops, maize and soybean. Currently, prevention and control of A. lucorum rely mainly on chemical pesticides that cause environmental as well as health related problems. Plant-mediated RNAi has proven to offer great potential for pest control in the past decade. In this study, we screened and obtained seven candidate genes (Alucβ-actin, AlucV-ATPase-A/D/E, AlucEif5A, AlucEcR-A, AlucIAP) by injection-based RNAi which produced A. lucorum mortality rates of 46.01–82.32% at day 7 after injection. Among them, the plant-mediated RNAi of AlucV-ATPase-E was successfully introduced into transgenic maize and soybean, and the populations of A. lucorum were significantly decreased following feeding on the transgenic maize and soybean. These results are intended to provide helpful insight into the generation of bug-resistant plants through a plant-mediated RNAi strategy. |
format | Online Article Text |
id | pubmed-6374644 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-63746442019-02-21 Plant-Mediated RNAi for Controlling Apolygus lucorum Liu, Fangzhou Yang, Bin Zhang, Aihong Ding, Derong Wang, Guirong Front Plant Sci Plant Science The polyphagous mirid bug Apolygus lucorum (Heteroptera: Miridae) is a serious pest of agricultural crops in China, with more than 200 species of host plants including two very important crops, maize and soybean. Currently, prevention and control of A. lucorum rely mainly on chemical pesticides that cause environmental as well as health related problems. Plant-mediated RNAi has proven to offer great potential for pest control in the past decade. In this study, we screened and obtained seven candidate genes (Alucβ-actin, AlucV-ATPase-A/D/E, AlucEif5A, AlucEcR-A, AlucIAP) by injection-based RNAi which produced A. lucorum mortality rates of 46.01–82.32% at day 7 after injection. Among them, the plant-mediated RNAi of AlucV-ATPase-E was successfully introduced into transgenic maize and soybean, and the populations of A. lucorum were significantly decreased following feeding on the transgenic maize and soybean. These results are intended to provide helpful insight into the generation of bug-resistant plants through a plant-mediated RNAi strategy. Frontiers Media S.A. 2019-02-06 /pmc/articles/PMC6374644/ /pubmed/30792724 http://dx.doi.org/10.3389/fpls.2019.00064 Text en Copyright © 2019 Liu, Yang, Zhang, Ding and Wang. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Plant Science Liu, Fangzhou Yang, Bin Zhang, Aihong Ding, Derong Wang, Guirong Plant-Mediated RNAi for Controlling Apolygus lucorum |
title | Plant-Mediated RNAi for Controlling Apolygus lucorum |
title_full | Plant-Mediated RNAi for Controlling Apolygus lucorum |
title_fullStr | Plant-Mediated RNAi for Controlling Apolygus lucorum |
title_full_unstemmed | Plant-Mediated RNAi for Controlling Apolygus lucorum |
title_short | Plant-Mediated RNAi for Controlling Apolygus lucorum |
title_sort | plant-mediated rnai for controlling apolygus lucorum |
topic | Plant Science |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6374644/ https://www.ncbi.nlm.nih.gov/pubmed/30792724 http://dx.doi.org/10.3389/fpls.2019.00064 |
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