Cargando…

Plant-Mediated RNAi for Controlling Apolygus lucorum

The polyphagous mirid bug Apolygus lucorum (Heteroptera: Miridae) is a serious pest of agricultural crops in China, with more than 200 species of host plants including two very important crops, maize and soybean. Currently, prevention and control of A. lucorum rely mainly on chemical pesticides that...

Descripción completa

Detalles Bibliográficos
Autores principales: Liu, Fangzhou, Yang, Bin, Zhang, Aihong, Ding, Derong, Wang, Guirong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6374644/
https://www.ncbi.nlm.nih.gov/pubmed/30792724
http://dx.doi.org/10.3389/fpls.2019.00064
_version_ 1783395204134338560
author Liu, Fangzhou
Yang, Bin
Zhang, Aihong
Ding, Derong
Wang, Guirong
author_facet Liu, Fangzhou
Yang, Bin
Zhang, Aihong
Ding, Derong
Wang, Guirong
author_sort Liu, Fangzhou
collection PubMed
description The polyphagous mirid bug Apolygus lucorum (Heteroptera: Miridae) is a serious pest of agricultural crops in China, with more than 200 species of host plants including two very important crops, maize and soybean. Currently, prevention and control of A. lucorum rely mainly on chemical pesticides that cause environmental as well as health related problems. Plant-mediated RNAi has proven to offer great potential for pest control in the past decade. In this study, we screened and obtained seven candidate genes (Alucβ-actin, AlucV-ATPase-A/D/E, AlucEif5A, AlucEcR-A, AlucIAP) by injection-based RNAi which produced A. lucorum mortality rates of 46.01–82.32% at day 7 after injection. Among them, the plant-mediated RNAi of AlucV-ATPase-E was successfully introduced into transgenic maize and soybean, and the populations of A. lucorum were significantly decreased following feeding on the transgenic maize and soybean. These results are intended to provide helpful insight into the generation of bug-resistant plants through a plant-mediated RNAi strategy.
format Online
Article
Text
id pubmed-6374644
institution National Center for Biotechnology Information
language English
publishDate 2019
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-63746442019-02-21 Plant-Mediated RNAi for Controlling Apolygus lucorum Liu, Fangzhou Yang, Bin Zhang, Aihong Ding, Derong Wang, Guirong Front Plant Sci Plant Science The polyphagous mirid bug Apolygus lucorum (Heteroptera: Miridae) is a serious pest of agricultural crops in China, with more than 200 species of host plants including two very important crops, maize and soybean. Currently, prevention and control of A. lucorum rely mainly on chemical pesticides that cause environmental as well as health related problems. Plant-mediated RNAi has proven to offer great potential for pest control in the past decade. In this study, we screened and obtained seven candidate genes (Alucβ-actin, AlucV-ATPase-A/D/E, AlucEif5A, AlucEcR-A, AlucIAP) by injection-based RNAi which produced A. lucorum mortality rates of 46.01–82.32% at day 7 after injection. Among them, the plant-mediated RNAi of AlucV-ATPase-E was successfully introduced into transgenic maize and soybean, and the populations of A. lucorum were significantly decreased following feeding on the transgenic maize and soybean. These results are intended to provide helpful insight into the generation of bug-resistant plants through a plant-mediated RNAi strategy. Frontiers Media S.A. 2019-02-06 /pmc/articles/PMC6374644/ /pubmed/30792724 http://dx.doi.org/10.3389/fpls.2019.00064 Text en Copyright © 2019 Liu, Yang, Zhang, Ding and Wang. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Liu, Fangzhou
Yang, Bin
Zhang, Aihong
Ding, Derong
Wang, Guirong
Plant-Mediated RNAi for Controlling Apolygus lucorum
title Plant-Mediated RNAi for Controlling Apolygus lucorum
title_full Plant-Mediated RNAi for Controlling Apolygus lucorum
title_fullStr Plant-Mediated RNAi for Controlling Apolygus lucorum
title_full_unstemmed Plant-Mediated RNAi for Controlling Apolygus lucorum
title_short Plant-Mediated RNAi for Controlling Apolygus lucorum
title_sort plant-mediated rnai for controlling apolygus lucorum
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6374644/
https://www.ncbi.nlm.nih.gov/pubmed/30792724
http://dx.doi.org/10.3389/fpls.2019.00064
work_keys_str_mv AT liufangzhou plantmediatedrnaiforcontrollingapolyguslucorum
AT yangbin plantmediatedrnaiforcontrollingapolyguslucorum
AT zhangaihong plantmediatedrnaiforcontrollingapolyguslucorum
AT dingderong plantmediatedrnaiforcontrollingapolyguslucorum
AT wangguirong plantmediatedrnaiforcontrollingapolyguslucorum