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An epidemiological investigation of porcine circovirus 3 infection in cattle in Shandong province, China
BACKGROUND: Porcine circovirus type 3 (PCV3) is a single-stranded, closed circular DNA virus, which causes porcine dermatitis and nephropathy syndrome (PDNS), multisystemic inflammation, and reproductive failure. The present study aimed to investigate the seroprevalence of PCV3 in cattle (Bos taurus...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6375139/ https://www.ncbi.nlm.nih.gov/pubmed/30760271 http://dx.doi.org/10.1186/s12917-019-1793-0 |
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author | Wang, Wei Sun, Wenchao Cao, Liang Zheng, Min Zhu, Yilong Li, Wenjie Liu, Cunxia Zhuang, Xinyu Xing, Jialiang Lu, Huijun Luo, Tingrong Jin, Ningyi |
author_facet | Wang, Wei Sun, Wenchao Cao, Liang Zheng, Min Zhu, Yilong Li, Wenjie Liu, Cunxia Zhuang, Xinyu Xing, Jialiang Lu, Huijun Luo, Tingrong Jin, Ningyi |
author_sort | Wang, Wei |
collection | PubMed |
description | BACKGROUND: Porcine circovirus type 3 (PCV3) is a single-stranded, closed circular DNA virus, which causes porcine dermatitis and nephropathy syndrome (PDNS), multisystemic inflammation, and reproductive failure. The present study aimed to investigate the seroprevalence of PCV3 in cattle (Bos taurus) in Shandong province, China, and examine its genome diversity. RESULTS: PCR amplification and sequencing showed that 74 of 213 bovine samples (34.7%) were positive for PCV3. Among them, the capsid gene (n = 12) and the complete genome (n = 4) were sequenced. These sequences had high identities to the reference capsid gene (98.0–100%) and the complete genome (97.5–99.8%). The PCV3 strains were classified into two different genotypes (PCV3a and PCV3b), according to phylogenetic analysis based on the complete genome and capsid gene sequences. Specifically, the bovine-origin strains in this study were grouped into PCV3a, showing a close relationship with PCV3-US/SD2016 (American strain; GenBank: KX966193.1). Notably, a comparison of the inferred amino acid sequences revealed a mutation from D124 to Y124. CONCLUSION: This was the first seroprevalence and genetic investigation of PCV3 in cattle in Shandong province, China. The results could provide insights into the epidemiology and pathogenesis of this important virus. |
format | Online Article Text |
id | pubmed-6375139 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-63751392019-02-26 An epidemiological investigation of porcine circovirus 3 infection in cattle in Shandong province, China Wang, Wei Sun, Wenchao Cao, Liang Zheng, Min Zhu, Yilong Li, Wenjie Liu, Cunxia Zhuang, Xinyu Xing, Jialiang Lu, Huijun Luo, Tingrong Jin, Ningyi BMC Vet Res Research Article BACKGROUND: Porcine circovirus type 3 (PCV3) is a single-stranded, closed circular DNA virus, which causes porcine dermatitis and nephropathy syndrome (PDNS), multisystemic inflammation, and reproductive failure. The present study aimed to investigate the seroprevalence of PCV3 in cattle (Bos taurus) in Shandong province, China, and examine its genome diversity. RESULTS: PCR amplification and sequencing showed that 74 of 213 bovine samples (34.7%) were positive for PCV3. Among them, the capsid gene (n = 12) and the complete genome (n = 4) were sequenced. These sequences had high identities to the reference capsid gene (98.0–100%) and the complete genome (97.5–99.8%). The PCV3 strains were classified into two different genotypes (PCV3a and PCV3b), according to phylogenetic analysis based on the complete genome and capsid gene sequences. Specifically, the bovine-origin strains in this study were grouped into PCV3a, showing a close relationship with PCV3-US/SD2016 (American strain; GenBank: KX966193.1). Notably, a comparison of the inferred amino acid sequences revealed a mutation from D124 to Y124. CONCLUSION: This was the first seroprevalence and genetic investigation of PCV3 in cattle in Shandong province, China. The results could provide insights into the epidemiology and pathogenesis of this important virus. BioMed Central 2019-02-13 /pmc/articles/PMC6375139/ /pubmed/30760271 http://dx.doi.org/10.1186/s12917-019-1793-0 Text en © The Author(s). 2019 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Research Article Wang, Wei Sun, Wenchao Cao, Liang Zheng, Min Zhu, Yilong Li, Wenjie Liu, Cunxia Zhuang, Xinyu Xing, Jialiang Lu, Huijun Luo, Tingrong Jin, Ningyi An epidemiological investigation of porcine circovirus 3 infection in cattle in Shandong province, China |
title | An epidemiological investigation of porcine circovirus 3 infection in cattle in Shandong province, China |
title_full | An epidemiological investigation of porcine circovirus 3 infection in cattle in Shandong province, China |
title_fullStr | An epidemiological investigation of porcine circovirus 3 infection in cattle in Shandong province, China |
title_full_unstemmed | An epidemiological investigation of porcine circovirus 3 infection in cattle in Shandong province, China |
title_short | An epidemiological investigation of porcine circovirus 3 infection in cattle in Shandong province, China |
title_sort | epidemiological investigation of porcine circovirus 3 infection in cattle in shandong province, china |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6375139/ https://www.ncbi.nlm.nih.gov/pubmed/30760271 http://dx.doi.org/10.1186/s12917-019-1793-0 |
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