Cargando…

A validation study of loci associated with mastitis resistance in two French dairy sheep breeds

BACKGROUND: The identification of loci associated with resistance to mastitis or of the causative mutations may be helpful in breeding programs for dairy sheep as it is for cattle worldwide. Seven genomic regions that control milk somatic cell counts, an indirect indicator of udder infection, have a...

Descripción completa

Detalles Bibliográficos
Autores principales: Oget, Claire, Allain, Charlotte, Portes, David, Foucras, Gilles, Stella, Alessandra, Astruc, Jean-Michel, Sarry, Julien, Tosser-Klopp, Gwenola, Rupp, Rachel
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6375205/
https://www.ncbi.nlm.nih.gov/pubmed/30760210
http://dx.doi.org/10.1186/s12711-019-0448-8
_version_ 1783395333764546560
author Oget, Claire
Allain, Charlotte
Portes, David
Foucras, Gilles
Stella, Alessandra
Astruc, Jean-Michel
Sarry, Julien
Tosser-Klopp, Gwenola
Rupp, Rachel
author_facet Oget, Claire
Allain, Charlotte
Portes, David
Foucras, Gilles
Stella, Alessandra
Astruc, Jean-Michel
Sarry, Julien
Tosser-Klopp, Gwenola
Rupp, Rachel
author_sort Oget, Claire
collection PubMed
description BACKGROUND: The identification of loci associated with resistance to mastitis or of the causative mutations may be helpful in breeding programs for dairy sheep as it is for cattle worldwide. Seven genomic regions that control milk somatic cell counts, an indirect indicator of udder infection, have already been identified in sheep (Spanish Churra, French Lacaune and Italian Sardinian–Lacaune backcross populations). In this study, we used a 960 custom-designed ovine single nucleotide polymorphism (SNP) chip in Lacaune and Manech Tête Rousse dairy sheep to validate these seven genomic regions associated with mastitis. RESULTS: The most significant SNP (rs868996547) on Ovis aries chromosome (OAR) 3 was a previously described mutation in the suppressor of cytokine signalling 2 (SOCS2) gene. An antagonist effect of this causal candidate between health and growth in Lacaune sheep was confirmed. Effects of the mutation on the infectious status of the udder, i.e. increases in milk somatic cell counts and bacteria shedding, were also identified. This SNP was not present in the data available on Manech Tête Rousse. Three other regions associated with mastitis were also confirmed on OAR16 (Manech Tête Rousse), 19 (Lacaune) and 2 (both breeds). For the OAR2 region, we validated previously detected SNPs in several other breeds (Sarda, Churra, and Chios). For significant SNPs in the four mastitis regions, the effect varied from 0.24 to 0.67 phenotypic standard deviation of the traits. Two of the mastitis quantitative trait loci (QTL) regions (OAR2 and 16) that we validated here were also associated in opposite ways with milk production traits in both populations. CONCLUSIONS: These results indicate, at least in part, a genomic basis for the trade-off between milk production and mastitis resistance. Four of the seven mastitis QTL regions that were previously identified in independent populations, were confirmed in this study, which demonstrates partial sharing of mastitis-related genetic mechanisms between different distant dairy sheep populations.
format Online
Article
Text
id pubmed-6375205
institution National Center for Biotechnology Information
language English
publishDate 2019
publisher BioMed Central
record_format MEDLINE/PubMed
spelling pubmed-63752052019-02-26 A validation study of loci associated with mastitis resistance in two French dairy sheep breeds Oget, Claire Allain, Charlotte Portes, David Foucras, Gilles Stella, Alessandra Astruc, Jean-Michel Sarry, Julien Tosser-Klopp, Gwenola Rupp, Rachel Genet Sel Evol Short Communication BACKGROUND: The identification of loci associated with resistance to mastitis or of the causative mutations may be helpful in breeding programs for dairy sheep as it is for cattle worldwide. Seven genomic regions that control milk somatic cell counts, an indirect indicator of udder infection, have already been identified in sheep (Spanish Churra, French Lacaune and Italian Sardinian–Lacaune backcross populations). In this study, we used a 960 custom-designed ovine single nucleotide polymorphism (SNP) chip in Lacaune and Manech Tête Rousse dairy sheep to validate these seven genomic regions associated with mastitis. RESULTS: The most significant SNP (rs868996547) on Ovis aries chromosome (OAR) 3 was a previously described mutation in the suppressor of cytokine signalling 2 (SOCS2) gene. An antagonist effect of this causal candidate between health and growth in Lacaune sheep was confirmed. Effects of the mutation on the infectious status of the udder, i.e. increases in milk somatic cell counts and bacteria shedding, were also identified. This SNP was not present in the data available on Manech Tête Rousse. Three other regions associated with mastitis were also confirmed on OAR16 (Manech Tête Rousse), 19 (Lacaune) and 2 (both breeds). For the OAR2 region, we validated previously detected SNPs in several other breeds (Sarda, Churra, and Chios). For significant SNPs in the four mastitis regions, the effect varied from 0.24 to 0.67 phenotypic standard deviation of the traits. Two of the mastitis quantitative trait loci (QTL) regions (OAR2 and 16) that we validated here were also associated in opposite ways with milk production traits in both populations. CONCLUSIONS: These results indicate, at least in part, a genomic basis for the trade-off between milk production and mastitis resistance. Four of the seven mastitis QTL regions that were previously identified in independent populations, were confirmed in this study, which demonstrates partial sharing of mastitis-related genetic mechanisms between different distant dairy sheep populations. BioMed Central 2019-02-13 /pmc/articles/PMC6375205/ /pubmed/30760210 http://dx.doi.org/10.1186/s12711-019-0448-8 Text en © The Author(s) 2019 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Short Communication
Oget, Claire
Allain, Charlotte
Portes, David
Foucras, Gilles
Stella, Alessandra
Astruc, Jean-Michel
Sarry, Julien
Tosser-Klopp, Gwenola
Rupp, Rachel
A validation study of loci associated with mastitis resistance in two French dairy sheep breeds
title A validation study of loci associated with mastitis resistance in two French dairy sheep breeds
title_full A validation study of loci associated with mastitis resistance in two French dairy sheep breeds
title_fullStr A validation study of loci associated with mastitis resistance in two French dairy sheep breeds
title_full_unstemmed A validation study of loci associated with mastitis resistance in two French dairy sheep breeds
title_short A validation study of loci associated with mastitis resistance in two French dairy sheep breeds
title_sort validation study of loci associated with mastitis resistance in two french dairy sheep breeds
topic Short Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6375205/
https://www.ncbi.nlm.nih.gov/pubmed/30760210
http://dx.doi.org/10.1186/s12711-019-0448-8
work_keys_str_mv AT ogetclaire avalidationstudyoflociassociatedwithmastitisresistanceintwofrenchdairysheepbreeds
AT allaincharlotte avalidationstudyoflociassociatedwithmastitisresistanceintwofrenchdairysheepbreeds
AT portesdavid avalidationstudyoflociassociatedwithmastitisresistanceintwofrenchdairysheepbreeds
AT foucrasgilles avalidationstudyoflociassociatedwithmastitisresistanceintwofrenchdairysheepbreeds
AT stellaalessandra avalidationstudyoflociassociatedwithmastitisresistanceintwofrenchdairysheepbreeds
AT astrucjeanmichel avalidationstudyoflociassociatedwithmastitisresistanceintwofrenchdairysheepbreeds
AT sarryjulien avalidationstudyoflociassociatedwithmastitisresistanceintwofrenchdairysheepbreeds
AT tosserkloppgwenola avalidationstudyoflociassociatedwithmastitisresistanceintwofrenchdairysheepbreeds
AT rupprachel avalidationstudyoflociassociatedwithmastitisresistanceintwofrenchdairysheepbreeds
AT ogetclaire validationstudyoflociassociatedwithmastitisresistanceintwofrenchdairysheepbreeds
AT allaincharlotte validationstudyoflociassociatedwithmastitisresistanceintwofrenchdairysheepbreeds
AT portesdavid validationstudyoflociassociatedwithmastitisresistanceintwofrenchdairysheepbreeds
AT foucrasgilles validationstudyoflociassociatedwithmastitisresistanceintwofrenchdairysheepbreeds
AT stellaalessandra validationstudyoflociassociatedwithmastitisresistanceintwofrenchdairysheepbreeds
AT astrucjeanmichel validationstudyoflociassociatedwithmastitisresistanceintwofrenchdairysheepbreeds
AT sarryjulien validationstudyoflociassociatedwithmastitisresistanceintwofrenchdairysheepbreeds
AT tosserkloppgwenola validationstudyoflociassociatedwithmastitisresistanceintwofrenchdairysheepbreeds
AT rupprachel validationstudyoflociassociatedwithmastitisresistanceintwofrenchdairysheepbreeds