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Spatiotemporal expression of the putative MdtABC efflux pump of Phtotorhabdus luminescens occurs in a protease-dependent manner during insect infection

Photorhabdus luminescens is an enterobacterium establishing a mutualistic symbiosis with nematodes, that also kills insects after septicaemia and connective tissue colonization. The role of the bacterial mdtABC genes encoding a putative multidrug efflux system from the resistance/nodulation/cell div...

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Autores principales: Abi Khattar, Ziad, Lanois, Anne, Hadchity, Linda, Gaudriault, Sophie, Givaudan, Alain
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6375597/
https://www.ncbi.nlm.nih.gov/pubmed/30763358
http://dx.doi.org/10.1371/journal.pone.0212077
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author Abi Khattar, Ziad
Lanois, Anne
Hadchity, Linda
Gaudriault, Sophie
Givaudan, Alain
author_facet Abi Khattar, Ziad
Lanois, Anne
Hadchity, Linda
Gaudriault, Sophie
Givaudan, Alain
author_sort Abi Khattar, Ziad
collection PubMed
description Photorhabdus luminescens is an enterobacterium establishing a mutualistic symbiosis with nematodes, that also kills insects after septicaemia and connective tissue colonization. The role of the bacterial mdtABC genes encoding a putative multidrug efflux system from the resistance/nodulation/cell division family was investigated. We showed that a mdtA mutant and the wild type had similar levels of resistance to antibiotics, antimicrobial peptides, metals, detergents and bile salts. The mdtA mutant was also as pathogenic as the wild-type following intrahaemocoel injection in Locusta migratoria, but had a slightly attenuated phenotype in Spodoptera littoralis. A transcriptional fusion of the mdtA promoter (P(mdtA)) and the green fluorescent protein (gfp) encoding gene was induced by copper in bacteria cultured in vitro. The P(mdtA)-gfp fusion was strongly induced within bacterial aggregates in the haematopoietic organ during late stages of infection in L. migratoria, whereas it was only weakly expressed in insect plasma throughout infection. A medium supplemented with haematopoietic organ extracts induced the P(mdtA)-gfp fusion ex vivo, suggesting that site-specific mdtABC expression resulted from insect signals from the haematopoietic organ. Finally, we showed that protease inhibitors abolished ex vivo activity of the P(mdtA)-gfp fusion in the presence of haematopoietic organ extracts, suggesting that proteolysis by-products play a key role in upregulating the putative MdtABC efflux pump during insect infection with P. luminescens.
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spelling pubmed-63755972019-03-01 Spatiotemporal expression of the putative MdtABC efflux pump of Phtotorhabdus luminescens occurs in a protease-dependent manner during insect infection Abi Khattar, Ziad Lanois, Anne Hadchity, Linda Gaudriault, Sophie Givaudan, Alain PLoS One Research Article Photorhabdus luminescens is an enterobacterium establishing a mutualistic symbiosis with nematodes, that also kills insects after septicaemia and connective tissue colonization. The role of the bacterial mdtABC genes encoding a putative multidrug efflux system from the resistance/nodulation/cell division family was investigated. We showed that a mdtA mutant and the wild type had similar levels of resistance to antibiotics, antimicrobial peptides, metals, detergents and bile salts. The mdtA mutant was also as pathogenic as the wild-type following intrahaemocoel injection in Locusta migratoria, but had a slightly attenuated phenotype in Spodoptera littoralis. A transcriptional fusion of the mdtA promoter (P(mdtA)) and the green fluorescent protein (gfp) encoding gene was induced by copper in bacteria cultured in vitro. The P(mdtA)-gfp fusion was strongly induced within bacterial aggregates in the haematopoietic organ during late stages of infection in L. migratoria, whereas it was only weakly expressed in insect plasma throughout infection. A medium supplemented with haematopoietic organ extracts induced the P(mdtA)-gfp fusion ex vivo, suggesting that site-specific mdtABC expression resulted from insect signals from the haematopoietic organ. Finally, we showed that protease inhibitors abolished ex vivo activity of the P(mdtA)-gfp fusion in the presence of haematopoietic organ extracts, suggesting that proteolysis by-products play a key role in upregulating the putative MdtABC efflux pump during insect infection with P. luminescens. Public Library of Science 2019-02-14 /pmc/articles/PMC6375597/ /pubmed/30763358 http://dx.doi.org/10.1371/journal.pone.0212077 Text en © 2019 Abi Khattar et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Abi Khattar, Ziad
Lanois, Anne
Hadchity, Linda
Gaudriault, Sophie
Givaudan, Alain
Spatiotemporal expression of the putative MdtABC efflux pump of Phtotorhabdus luminescens occurs in a protease-dependent manner during insect infection
title Spatiotemporal expression of the putative MdtABC efflux pump of Phtotorhabdus luminescens occurs in a protease-dependent manner during insect infection
title_full Spatiotemporal expression of the putative MdtABC efflux pump of Phtotorhabdus luminescens occurs in a protease-dependent manner during insect infection
title_fullStr Spatiotemporal expression of the putative MdtABC efflux pump of Phtotorhabdus luminescens occurs in a protease-dependent manner during insect infection
title_full_unstemmed Spatiotemporal expression of the putative MdtABC efflux pump of Phtotorhabdus luminescens occurs in a protease-dependent manner during insect infection
title_short Spatiotemporal expression of the putative MdtABC efflux pump of Phtotorhabdus luminescens occurs in a protease-dependent manner during insect infection
title_sort spatiotemporal expression of the putative mdtabc efflux pump of phtotorhabdus luminescens occurs in a protease-dependent manner during insect infection
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6375597/
https://www.ncbi.nlm.nih.gov/pubmed/30763358
http://dx.doi.org/10.1371/journal.pone.0212077
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