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The emergence of pathogenic TNF/iNOS producing dendritic cells (Tip-DCs) in a malaria model of acute respiratory distress syndrome (ARDS) is dependent on CCR4
Malaria-associated acute respiratory distress syndrome (MA-ARDS) and acute lung injury (ALI) are complications that cause lung damage and often leads to death. The MA-ARDS/ALI is associated with a Type 1 inflammatory response mediated by T lymphocytes and IFN-γ. Here, we used the Plasmodium berghei...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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© Society for Mucosal Immunology. Published by Elsevier Inc.
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6375779/ https://www.ncbi.nlm.nih.gov/pubmed/30337650 http://dx.doi.org/10.1038/s41385-018-0093-5 |
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author | Galvão-Filho, Bruno de Castro, Júlia Teixeira Figueiredo, Maria Marta Rosmaninho, Claudio Gonçalves Antonelli, Lis Ribeiro do Valle Gazzinelli, Ricardo Tostes |
author_facet | Galvão-Filho, Bruno de Castro, Júlia Teixeira Figueiredo, Maria Marta Rosmaninho, Claudio Gonçalves Antonelli, Lis Ribeiro do Valle Gazzinelli, Ricardo Tostes |
author_sort | Galvão-Filho, Bruno |
collection | PubMed |
description | Malaria-associated acute respiratory distress syndrome (MA-ARDS) and acute lung injury (ALI) are complications that cause lung damage and often leads to death. The MA-ARDS/ALI is associated with a Type 1 inflammatory response mediated by T lymphocytes and IFN-γ. Here, we used the Plasmodium berghei NK65 (PbN)-induced MA-ALI/ARDS model that resembles human disease and confirmed that lung CD4(+) and CD8(+) T cells predominantly expressed Tbet and IFN-γ. Surprisingly, we found that development of MA-ALI/ARDS was dependent on functional CCR4, known to mediate the recruitment of Th2 lymphocytes and regulatory T cells. However, in this Type 1 inflammation-ARDS model, CCR4 was not involved in the recruitment of T lymphocytes, but was required for the emergence of TNF-α/iNOS producing dendritic cells (Tip-DCs) in the lungs. In contrast, recruitment of Tip-DCs and development of MA-ALI/ARDS were not altered in CCR2(−/)(−) mice. Importantly, we showed that NOS2(−/)(−) mice are resistant to PbN-induced lung damage, indicating that reactive nitrogen species produced by Tip-DCs play an essential role in inducing MA-ARDS/ALI. Lastly, our experiments suggest that production of IFN-γ primarily by CD8(+) T cells is required for inducing Tip-DCs differentiation in the lungs and the development of MA-ALI/ARDS model. |
format | Online Article Text |
id | pubmed-6375779 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | © Society for Mucosal Immunology. Published by Elsevier Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-63757792019-04-18 The emergence of pathogenic TNF/iNOS producing dendritic cells (Tip-DCs) in a malaria model of acute respiratory distress syndrome (ARDS) is dependent on CCR4 Galvão-Filho, Bruno de Castro, Júlia Teixeira Figueiredo, Maria Marta Rosmaninho, Claudio Gonçalves Antonelli, Lis Ribeiro do Valle Gazzinelli, Ricardo Tostes Mucosal Immunol Article Malaria-associated acute respiratory distress syndrome (MA-ARDS) and acute lung injury (ALI) are complications that cause lung damage and often leads to death. The MA-ARDS/ALI is associated with a Type 1 inflammatory response mediated by T lymphocytes and IFN-γ. Here, we used the Plasmodium berghei NK65 (PbN)-induced MA-ALI/ARDS model that resembles human disease and confirmed that lung CD4(+) and CD8(+) T cells predominantly expressed Tbet and IFN-γ. Surprisingly, we found that development of MA-ALI/ARDS was dependent on functional CCR4, known to mediate the recruitment of Th2 lymphocytes and regulatory T cells. However, in this Type 1 inflammation-ARDS model, CCR4 was not involved in the recruitment of T lymphocytes, but was required for the emergence of TNF-α/iNOS producing dendritic cells (Tip-DCs) in the lungs. In contrast, recruitment of Tip-DCs and development of MA-ALI/ARDS were not altered in CCR2(−/)(−) mice. Importantly, we showed that NOS2(−/)(−) mice are resistant to PbN-induced lung damage, indicating that reactive nitrogen species produced by Tip-DCs play an essential role in inducing MA-ARDS/ALI. Lastly, our experiments suggest that production of IFN-γ primarily by CD8(+) T cells is required for inducing Tip-DCs differentiation in the lungs and the development of MA-ALI/ARDS model. © Society for Mucosal Immunology. Published by Elsevier Inc. 2019-03 2022-12-31 /pmc/articles/PMC6375779/ /pubmed/30337650 http://dx.doi.org/10.1038/s41385-018-0093-5 Text en Copyright © 2018 © Society for Mucosal Immunology. Published by Elsevier Inc. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active. |
spellingShingle | Article Galvão-Filho, Bruno de Castro, Júlia Teixeira Figueiredo, Maria Marta Rosmaninho, Claudio Gonçalves Antonelli, Lis Ribeiro do Valle Gazzinelli, Ricardo Tostes The emergence of pathogenic TNF/iNOS producing dendritic cells (Tip-DCs) in a malaria model of acute respiratory distress syndrome (ARDS) is dependent on CCR4 |
title | The emergence of pathogenic TNF/iNOS producing dendritic cells (Tip-DCs) in a malaria model of acute respiratory distress syndrome (ARDS) is dependent on CCR4 |
title_full | The emergence of pathogenic TNF/iNOS producing dendritic cells (Tip-DCs) in a malaria model of acute respiratory distress syndrome (ARDS) is dependent on CCR4 |
title_fullStr | The emergence of pathogenic TNF/iNOS producing dendritic cells (Tip-DCs) in a malaria model of acute respiratory distress syndrome (ARDS) is dependent on CCR4 |
title_full_unstemmed | The emergence of pathogenic TNF/iNOS producing dendritic cells (Tip-DCs) in a malaria model of acute respiratory distress syndrome (ARDS) is dependent on CCR4 |
title_short | The emergence of pathogenic TNF/iNOS producing dendritic cells (Tip-DCs) in a malaria model of acute respiratory distress syndrome (ARDS) is dependent on CCR4 |
title_sort | emergence of pathogenic tnf/inos producing dendritic cells (tip-dcs) in a malaria model of acute respiratory distress syndrome (ards) is dependent on ccr4 |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6375779/ https://www.ncbi.nlm.nih.gov/pubmed/30337650 http://dx.doi.org/10.1038/s41385-018-0093-5 |
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