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Pre-analytical parameters associated with unsuccessful karyotyping in myeloid neoplasm: a study of 421 samples

Cytogenetics is essential in myeloid neoplasms (MN) and pre-analytical variables are important for karyotyping. We assessed the relationship between pre-analytical variables (time from collection to sample processing, material type, sample cellularity, and diagnosis) and failures of karyotyping. Bon...

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Autores principales: Santos, M.F.M., Oliveira, F.C.A.C., Kishimoto, R.K., Borri, D., Santos, F.P.S., Campregher, P.V., Silveira, P.A.A., Hamerschlak, N., Mangueira, C.L.P., Duarte, F.B., Crepaldi, A.H., Salvino, M.A., Velloso, E.D.R.P.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Associação Brasileira de Divulgação Científica 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6376323/
https://www.ncbi.nlm.nih.gov/pubmed/30785480
http://dx.doi.org/10.1590/1414-431X20188194
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author Santos, M.F.M.
Oliveira, F.C.A.C.
Kishimoto, R.K.
Borri, D.
Santos, F.P.S.
Campregher, P.V.
Silveira, P.A.A.
Hamerschlak, N.
Mangueira, C.L.P.
Duarte, F.B.
Crepaldi, A.H.
Salvino, M.A.
Velloso, E.D.R.P.
author_facet Santos, M.F.M.
Oliveira, F.C.A.C.
Kishimoto, R.K.
Borri, D.
Santos, F.P.S.
Campregher, P.V.
Silveira, P.A.A.
Hamerschlak, N.
Mangueira, C.L.P.
Duarte, F.B.
Crepaldi, A.H.
Salvino, M.A.
Velloso, E.D.R.P.
author_sort Santos, M.F.M.
collection PubMed
description Cytogenetics is essential in myeloid neoplasms (MN) and pre-analytical variables are important for karyotyping. We assessed the relationship between pre-analytical variables (time from collection to sample processing, material type, sample cellularity, and diagnosis) and failures of karyotyping. Bone marrow (BM, n=352) and peripheral blood (PB, n=69) samples were analyzed from acute myeloid leukemia (n=113), myelodysplastic syndromes (n=73), myelodysplastic syndromes/myeloproliferative neoplasms (n=17), myeloproliferative neoplasms (n=137), and other with conclusive diagnosis (n=6), and reactive disorders/no conclusive diagnosis (n=75). The rate of unsuccessful karyotyping was 18.5% and was associated with the use of PB and a low number of nucleated cells (≤7×10(3)/µL) in the sample. High and low cellularity in BM and high and low cellularity in PB samples showed no metaphases in 3.9, 39.7, 41.9, and 84.6% of cases, respectively. Collecting a good BM sample is the key for the success of karyotyping in MN and avoids the use of expensive molecular techniques.
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spelling pubmed-63763232019-03-06 Pre-analytical parameters associated with unsuccessful karyotyping in myeloid neoplasm: a study of 421 samples Santos, M.F.M. Oliveira, F.C.A.C. Kishimoto, R.K. Borri, D. Santos, F.P.S. Campregher, P.V. Silveira, P.A.A. Hamerschlak, N. Mangueira, C.L.P. Duarte, F.B. Crepaldi, A.H. Salvino, M.A. Velloso, E.D.R.P. Braz J Med Biol Res Research Article Cytogenetics is essential in myeloid neoplasms (MN) and pre-analytical variables are important for karyotyping. We assessed the relationship between pre-analytical variables (time from collection to sample processing, material type, sample cellularity, and diagnosis) and failures of karyotyping. Bone marrow (BM, n=352) and peripheral blood (PB, n=69) samples were analyzed from acute myeloid leukemia (n=113), myelodysplastic syndromes (n=73), myelodysplastic syndromes/myeloproliferative neoplasms (n=17), myeloproliferative neoplasms (n=137), and other with conclusive diagnosis (n=6), and reactive disorders/no conclusive diagnosis (n=75). The rate of unsuccessful karyotyping was 18.5% and was associated with the use of PB and a low number of nucleated cells (≤7×10(3)/µL) in the sample. High and low cellularity in BM and high and low cellularity in PB samples showed no metaphases in 3.9, 39.7, 41.9, and 84.6% of cases, respectively. Collecting a good BM sample is the key for the success of karyotyping in MN and avoids the use of expensive molecular techniques. Associação Brasileira de Divulgação Científica 2019-02-14 /pmc/articles/PMC6376323/ /pubmed/30785480 http://dx.doi.org/10.1590/1414-431X20188194 Text en https://creativecommons.org/licenses/by/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License, which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Santos, M.F.M.
Oliveira, F.C.A.C.
Kishimoto, R.K.
Borri, D.
Santos, F.P.S.
Campregher, P.V.
Silveira, P.A.A.
Hamerschlak, N.
Mangueira, C.L.P.
Duarte, F.B.
Crepaldi, A.H.
Salvino, M.A.
Velloso, E.D.R.P.
Pre-analytical parameters associated with unsuccessful karyotyping in myeloid neoplasm: a study of 421 samples
title Pre-analytical parameters associated with unsuccessful karyotyping in myeloid neoplasm: a study of 421 samples
title_full Pre-analytical parameters associated with unsuccessful karyotyping in myeloid neoplasm: a study of 421 samples
title_fullStr Pre-analytical parameters associated with unsuccessful karyotyping in myeloid neoplasm: a study of 421 samples
title_full_unstemmed Pre-analytical parameters associated with unsuccessful karyotyping in myeloid neoplasm: a study of 421 samples
title_short Pre-analytical parameters associated with unsuccessful karyotyping in myeloid neoplasm: a study of 421 samples
title_sort pre-analytical parameters associated with unsuccessful karyotyping in myeloid neoplasm: a study of 421 samples
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6376323/
https://www.ncbi.nlm.nih.gov/pubmed/30785480
http://dx.doi.org/10.1590/1414-431X20188194
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