Cargando…
Enhanced resistance to bacterial and oomycete pathogens by short tandem target mimic RNAs in tomato
Nucleotide binding site leucine-rich repeat (NLR) proteins of the plant innate immune system are negatively regulated by the miR482/2118 family miRNAs that are in a distinct 22-nt class of miRNAs with a double mode of action. First, they cleave the target RNA, as with the canonical 21-nt miRNAs, and...
Autores principales: | , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
National Academy of Sciences
2019
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6377479/ https://www.ncbi.nlm.nih.gov/pubmed/30679269 http://dx.doi.org/10.1073/pnas.1814380116 |
_version_ | 1783395746856304640 |
---|---|
author | Canto-Pastor, Alex Santos, Bruno A. M. C. Valli, Adrian A. Summers, William Schornack, Sebastian Baulcombe, David C. |
author_facet | Canto-Pastor, Alex Santos, Bruno A. M. C. Valli, Adrian A. Summers, William Schornack, Sebastian Baulcombe, David C. |
author_sort | Canto-Pastor, Alex |
collection | PubMed |
description | Nucleotide binding site leucine-rich repeat (NLR) proteins of the plant innate immune system are negatively regulated by the miR482/2118 family miRNAs that are in a distinct 22-nt class of miRNAs with a double mode of action. First, they cleave the target RNA, as with the canonical 21-nt miRNAs, and second, they trigger secondary siRNA production using the target RNA as a template. Here, we address the extent to which the miR482/2118 family affects expression of NLR mRNAs and disease resistance. We show that structural differences of miR482/2118 family members in tomato (Solanum lycopersicum) are functionally significant. The predicted target of the miR482 subfamily is a conserved motif in multiple NLR mRNAs, whereas for miR2118b, it is a noncoding RNA target formed by rearrangement of several different NLR genes. From RNA sequencing and degradome data in lines expressing short tandem target mimic (STTM) RNAs of miR482/2118, we confirm the different targets of these miRNAs. The effect on NLR mRNA accumulation is slight, but nevertheless, the tomato STTM lines display enhanced resistance to infection with the oomycete and bacterial pathogens. These data implicate an RNA cascade of miRNAs and secondary siRNAs in the regulation of NLR RNAs and show that the encoded NLR proteins have a role in quantitative disease resistance in addition to dominant gene resistance that has been well characterized elsewhere. We also illustrate the use of STTM RNA in a biotechnological approach for enhancing quantitative disease resistance in highly bred cultivars. |
format | Online Article Text |
id | pubmed-6377479 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | National Academy of Sciences |
record_format | MEDLINE/PubMed |
spelling | pubmed-63774792019-02-19 Enhanced resistance to bacterial and oomycete pathogens by short tandem target mimic RNAs in tomato Canto-Pastor, Alex Santos, Bruno A. M. C. Valli, Adrian A. Summers, William Schornack, Sebastian Baulcombe, David C. Proc Natl Acad Sci U S A Biological Sciences Nucleotide binding site leucine-rich repeat (NLR) proteins of the plant innate immune system are negatively regulated by the miR482/2118 family miRNAs that are in a distinct 22-nt class of miRNAs with a double mode of action. First, they cleave the target RNA, as with the canonical 21-nt miRNAs, and second, they trigger secondary siRNA production using the target RNA as a template. Here, we address the extent to which the miR482/2118 family affects expression of NLR mRNAs and disease resistance. We show that structural differences of miR482/2118 family members in tomato (Solanum lycopersicum) are functionally significant. The predicted target of the miR482 subfamily is a conserved motif in multiple NLR mRNAs, whereas for miR2118b, it is a noncoding RNA target formed by rearrangement of several different NLR genes. From RNA sequencing and degradome data in lines expressing short tandem target mimic (STTM) RNAs of miR482/2118, we confirm the different targets of these miRNAs. The effect on NLR mRNA accumulation is slight, but nevertheless, the tomato STTM lines display enhanced resistance to infection with the oomycete and bacterial pathogens. These data implicate an RNA cascade of miRNAs and secondary siRNAs in the regulation of NLR RNAs and show that the encoded NLR proteins have a role in quantitative disease resistance in addition to dominant gene resistance that has been well characterized elsewhere. We also illustrate the use of STTM RNA in a biotechnological approach for enhancing quantitative disease resistance in highly bred cultivars. National Academy of Sciences 2019-02-12 2019-01-24 /pmc/articles/PMC6377479/ /pubmed/30679269 http://dx.doi.org/10.1073/pnas.1814380116 Text en Copyright © 2019 the Author(s). Published by PNAS. https://creativecommons.org/licenses/by-nc-nd/4.0/ This open access article is distributed under Creative Commons Attribution-NonCommercial-NoDerivatives License 4.0 (CC BY-NC-ND) (https://creativecommons.org/licenses/by-nc-nd/4.0/) . |
spellingShingle | Biological Sciences Canto-Pastor, Alex Santos, Bruno A. M. C. Valli, Adrian A. Summers, William Schornack, Sebastian Baulcombe, David C. Enhanced resistance to bacterial and oomycete pathogens by short tandem target mimic RNAs in tomato |
title | Enhanced resistance to bacterial and oomycete pathogens by short tandem target mimic RNAs in tomato |
title_full | Enhanced resistance to bacterial and oomycete pathogens by short tandem target mimic RNAs in tomato |
title_fullStr | Enhanced resistance to bacterial and oomycete pathogens by short tandem target mimic RNAs in tomato |
title_full_unstemmed | Enhanced resistance to bacterial and oomycete pathogens by short tandem target mimic RNAs in tomato |
title_short | Enhanced resistance to bacterial and oomycete pathogens by short tandem target mimic RNAs in tomato |
title_sort | enhanced resistance to bacterial and oomycete pathogens by short tandem target mimic rnas in tomato |
topic | Biological Sciences |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6377479/ https://www.ncbi.nlm.nih.gov/pubmed/30679269 http://dx.doi.org/10.1073/pnas.1814380116 |
work_keys_str_mv | AT cantopastoralex enhancedresistancetobacterialandoomycetepathogensbyshorttandemtargetmimicrnasintomato AT santosbrunoamc enhancedresistancetobacterialandoomycetepathogensbyshorttandemtargetmimicrnasintomato AT valliadriana enhancedresistancetobacterialandoomycetepathogensbyshorttandemtargetmimicrnasintomato AT summerswilliam enhancedresistancetobacterialandoomycetepathogensbyshorttandemtargetmimicrnasintomato AT schornacksebastian enhancedresistancetobacterialandoomycetepathogensbyshorttandemtargetmimicrnasintomato AT baulcombedavidc enhancedresistancetobacterialandoomycetepathogensbyshorttandemtargetmimicrnasintomato |