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Sirt3 suppresses calcium oxalate-induced renal tubular epithelial cell injury via modification of FoxO3a-mediated autophagy

High oxalic acid and calcium oxalate (CaOx)-induced renal tubular epithelial cell (TEC) injury plays a key role in nephrolithiasis. However, the mechanism remains unknown. Gene array analysis of the mice nephrolithiasis model indicated significant downregulation of sirtuin 3 (Sirt3) and activation o...

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Autores principales: Peng, Yonghan, Yang, Cheng, Shi, Xiaolei, Li, Ling, Dong, Hao, Liu, Changcheng, Fang, Ziyu, Wang, Zeyu, Ming, Shaoxiong, Liu, Min, Xie, Bin, Gao, Xiaofeng, Sun, Yinghao
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6377683/
https://www.ncbi.nlm.nih.gov/pubmed/30674870
http://dx.doi.org/10.1038/s41419-018-1169-6
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author Peng, Yonghan
Yang, Cheng
Shi, Xiaolei
Li, Ling
Dong, Hao
Liu, Changcheng
Fang, Ziyu
Wang, Zeyu
Ming, Shaoxiong
Liu, Min
Xie, Bin
Gao, Xiaofeng
Sun, Yinghao
author_facet Peng, Yonghan
Yang, Cheng
Shi, Xiaolei
Li, Ling
Dong, Hao
Liu, Changcheng
Fang, Ziyu
Wang, Zeyu
Ming, Shaoxiong
Liu, Min
Xie, Bin
Gao, Xiaofeng
Sun, Yinghao
author_sort Peng, Yonghan
collection PubMed
description High oxalic acid and calcium oxalate (CaOx)-induced renal tubular epithelial cell (TEC) injury plays a key role in nephrolithiasis. However, the mechanism remains unknown. Gene array analysis of the mice nephrolithiasis model indicated significant downregulation of sirtuin 3 (Sirt3) and activation of mitogen-activated protein kinase (MAPK) pathway. Kidney biopsy tissues of renal calculi patients also showed decreased Sirt3 expression. Silencing Sirt3 exacerbated oxidative stress and TEC death under CaOx stimulation. Restoring Sirt3 expression by overexpression or enhancing its activity protected renal function and reduced TEC death both in vitro and in vivo. Inhibiting the MAPK pathway resulted in upregulation of Sirt3 expression, preservation of renal function and decreased cell death both in vitro and in vivo. Furthermore, Sirt3 could upregulate FoxO3a activity post-translationally via deacetylation, dephosphorylation and deubiquitination. FoxO3a was found to interact with the promoter region of LC3B and to increase its expression, enhancing TEC autophagy and suppressing cell apoptosis and necrosis. Taken together, our results indicate that the MAPK/Sirt3/FoxO3a pathway modulates renal TEC death and autophagy in TEC injury.
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spelling pubmed-63776832019-02-19 Sirt3 suppresses calcium oxalate-induced renal tubular epithelial cell injury via modification of FoxO3a-mediated autophagy Peng, Yonghan Yang, Cheng Shi, Xiaolei Li, Ling Dong, Hao Liu, Changcheng Fang, Ziyu Wang, Zeyu Ming, Shaoxiong Liu, Min Xie, Bin Gao, Xiaofeng Sun, Yinghao Cell Death Dis Article High oxalic acid and calcium oxalate (CaOx)-induced renal tubular epithelial cell (TEC) injury plays a key role in nephrolithiasis. However, the mechanism remains unknown. Gene array analysis of the mice nephrolithiasis model indicated significant downregulation of sirtuin 3 (Sirt3) and activation of mitogen-activated protein kinase (MAPK) pathway. Kidney biopsy tissues of renal calculi patients also showed decreased Sirt3 expression. Silencing Sirt3 exacerbated oxidative stress and TEC death under CaOx stimulation. Restoring Sirt3 expression by overexpression or enhancing its activity protected renal function and reduced TEC death both in vitro and in vivo. Inhibiting the MAPK pathway resulted in upregulation of Sirt3 expression, preservation of renal function and decreased cell death both in vitro and in vivo. Furthermore, Sirt3 could upregulate FoxO3a activity post-translationally via deacetylation, dephosphorylation and deubiquitination. FoxO3a was found to interact with the promoter region of LC3B and to increase its expression, enhancing TEC autophagy and suppressing cell apoptosis and necrosis. Taken together, our results indicate that the MAPK/Sirt3/FoxO3a pathway modulates renal TEC death and autophagy in TEC injury. Nature Publishing Group UK 2019-01-15 /pmc/articles/PMC6377683/ /pubmed/30674870 http://dx.doi.org/10.1038/s41419-018-1169-6 Text en © The Author(s) 2019 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Peng, Yonghan
Yang, Cheng
Shi, Xiaolei
Li, Ling
Dong, Hao
Liu, Changcheng
Fang, Ziyu
Wang, Zeyu
Ming, Shaoxiong
Liu, Min
Xie, Bin
Gao, Xiaofeng
Sun, Yinghao
Sirt3 suppresses calcium oxalate-induced renal tubular epithelial cell injury via modification of FoxO3a-mediated autophagy
title Sirt3 suppresses calcium oxalate-induced renal tubular epithelial cell injury via modification of FoxO3a-mediated autophagy
title_full Sirt3 suppresses calcium oxalate-induced renal tubular epithelial cell injury via modification of FoxO3a-mediated autophagy
title_fullStr Sirt3 suppresses calcium oxalate-induced renal tubular epithelial cell injury via modification of FoxO3a-mediated autophagy
title_full_unstemmed Sirt3 suppresses calcium oxalate-induced renal tubular epithelial cell injury via modification of FoxO3a-mediated autophagy
title_short Sirt3 suppresses calcium oxalate-induced renal tubular epithelial cell injury via modification of FoxO3a-mediated autophagy
title_sort sirt3 suppresses calcium oxalate-induced renal tubular epithelial cell injury via modification of foxo3a-mediated autophagy
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6377683/
https://www.ncbi.nlm.nih.gov/pubmed/30674870
http://dx.doi.org/10.1038/s41419-018-1169-6
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