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Construction of consolidated bio-saccharification biocatalyst and process optimization for highly efficient lignocellulose solubilization

BACKGROUND: The industrial conversion of biomass to high-value biofuels and biochemical is mainly restricted by lignocellulose solubilization. Consolidated bio-saccharification (CBS) is considered a promising process for lignocellulose solubilization depending on whole-cell biocatalysts that simulta...

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Autores principales: Liu, Shiyue, Liu, Ya-Jun, Feng, Yingang, Li, Bin, Cui, Qiu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6378752/
https://www.ncbi.nlm.nih.gov/pubmed/30820245
http://dx.doi.org/10.1186/s13068-019-1374-2
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author Liu, Shiyue
Liu, Ya-Jun
Feng, Yingang
Li, Bin
Cui, Qiu
author_facet Liu, Shiyue
Liu, Ya-Jun
Feng, Yingang
Li, Bin
Cui, Qiu
author_sort Liu, Shiyue
collection PubMed
description BACKGROUND: The industrial conversion of biomass to high-value biofuels and biochemical is mainly restricted by lignocellulose solubilization. Consolidated bio-saccharification (CBS) is considered a promising process for lignocellulose solubilization depending on whole-cell biocatalysts that simultaneously perform effective cellulase production and hydrolysis. However, it usually takes a long time to reach a high saccharification level using the current CBS biocatalyst and process. RESULTS: To promote the saccharification efficiency and reduce the cost, a Clostridium thermocellum recombinant strain ∆pyrF::KBm was constructed as a new CBS biocatalyst in this study. The key CBS factors, including the medium, inoculum size and cultivation, and substrate load, were investigated and optimized. The saccharification process was also stimulated by adding free hemicellulases, suggesting the need to further enhance hemicellulase activity of the whole-cell catalyst. Under the optimal conditions, the CBS process was shortened by 50% with pretreated wheat straw as the substrate. The sugar yield reached 0.795 g/g and the saccharification level was 89.3%. CONCLUSIONS: This work provided a new biocatalyst and an optimized process of CBS and confirmed that CBS is a feasible strategy for cost-efficient solubilization of lignocellulose, which will greatly promote the industrial utilization of lignocellulosic biomass. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s13068-019-1374-2) contains supplementary material, which is available to authorized users.
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spelling pubmed-63787522019-02-28 Construction of consolidated bio-saccharification biocatalyst and process optimization for highly efficient lignocellulose solubilization Liu, Shiyue Liu, Ya-Jun Feng, Yingang Li, Bin Cui, Qiu Biotechnol Biofuels Research BACKGROUND: The industrial conversion of biomass to high-value biofuels and biochemical is mainly restricted by lignocellulose solubilization. Consolidated bio-saccharification (CBS) is considered a promising process for lignocellulose solubilization depending on whole-cell biocatalysts that simultaneously perform effective cellulase production and hydrolysis. However, it usually takes a long time to reach a high saccharification level using the current CBS biocatalyst and process. RESULTS: To promote the saccharification efficiency and reduce the cost, a Clostridium thermocellum recombinant strain ∆pyrF::KBm was constructed as a new CBS biocatalyst in this study. The key CBS factors, including the medium, inoculum size and cultivation, and substrate load, were investigated and optimized. The saccharification process was also stimulated by adding free hemicellulases, suggesting the need to further enhance hemicellulase activity of the whole-cell catalyst. Under the optimal conditions, the CBS process was shortened by 50% with pretreated wheat straw as the substrate. The sugar yield reached 0.795 g/g and the saccharification level was 89.3%. CONCLUSIONS: This work provided a new biocatalyst and an optimized process of CBS and confirmed that CBS is a feasible strategy for cost-efficient solubilization of lignocellulose, which will greatly promote the industrial utilization of lignocellulosic biomass. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s13068-019-1374-2) contains supplementary material, which is available to authorized users. BioMed Central 2019-02-18 /pmc/articles/PMC6378752/ /pubmed/30820245 http://dx.doi.org/10.1186/s13068-019-1374-2 Text en © The Author(s) 2019 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research
Liu, Shiyue
Liu, Ya-Jun
Feng, Yingang
Li, Bin
Cui, Qiu
Construction of consolidated bio-saccharification biocatalyst and process optimization for highly efficient lignocellulose solubilization
title Construction of consolidated bio-saccharification biocatalyst and process optimization for highly efficient lignocellulose solubilization
title_full Construction of consolidated bio-saccharification biocatalyst and process optimization for highly efficient lignocellulose solubilization
title_fullStr Construction of consolidated bio-saccharification biocatalyst and process optimization for highly efficient lignocellulose solubilization
title_full_unstemmed Construction of consolidated bio-saccharification biocatalyst and process optimization for highly efficient lignocellulose solubilization
title_short Construction of consolidated bio-saccharification biocatalyst and process optimization for highly efficient lignocellulose solubilization
title_sort construction of consolidated bio-saccharification biocatalyst and process optimization for highly efficient lignocellulose solubilization
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6378752/
https://www.ncbi.nlm.nih.gov/pubmed/30820245
http://dx.doi.org/10.1186/s13068-019-1374-2
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