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Simultaneous multiplexed amplicon sequencing and transcriptome profiling in single cells

We describe Droplet Assisted RNA Targeting by single cell sequencing (DART-seq), a versatile technology that enables multiplexed amplicon sequencing and transcriptome profiling in single cells. We applied DART-seq to simultaneously characterize the non-A-tailed transcripts of a segmented dsRNA virus...

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Detalles Bibliográficos
Autores principales: Saikia, Mridusmita, Burnham, Philip, Keshavjee, Sara H., Wang, Michael F. Z., Heyang, Michael, Moral-Lopez, Pablo, Hinchman, Meleana M., Danko, Charles G., Parker, John S. L., De Vlaminck, Iwijn
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6378878/
https://www.ncbi.nlm.nih.gov/pubmed/30559431
http://dx.doi.org/10.1038/s41592-018-0259-9
Descripción
Sumario:We describe Droplet Assisted RNA Targeting by single cell sequencing (DART-seq), a versatile technology that enables multiplexed amplicon sequencing and transcriptome profiling in single cells. We applied DART-seq to simultaneously characterize the non-A-tailed transcripts of a segmented dsRNA virus and the transcriptome of the infected cell. In addition, we used DART-seq to simultaneously determine the natively paired, variable region heavy and light chain amplicons and the transcriptome of B lymphocytes.