Simultaneous multiplexed amplicon sequencing and transcriptome profiling in single cells

We describe Droplet Assisted RNA Targeting by single cell sequencing (DART-seq), a versatile technology that enables multiplexed amplicon sequencing and transcriptome profiling in single cells. We applied DART-seq to simultaneously characterize the non-A-tailed transcripts of a segmented dsRNA virus...

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Autores principales: Saikia, Mridusmita, Burnham, Philip, Keshavjee, Sara H., Wang, Michael F. Z., Heyang, Michael, Moral-Lopez, Pablo, Hinchman, Meleana M., Danko, Charles G., Parker, John S. L., De Vlaminck, Iwijn
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2018
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6378878/
https://www.ncbi.nlm.nih.gov/pubmed/30559431
http://dx.doi.org/10.1038/s41592-018-0259-9
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author Saikia, Mridusmita
Burnham, Philip
Keshavjee, Sara H.
Wang, Michael F. Z.
Heyang, Michael
Moral-Lopez, Pablo
Hinchman, Meleana M.
Danko, Charles G.
Parker, John S. L.
De Vlaminck, Iwijn
author_facet Saikia, Mridusmita
Burnham, Philip
Keshavjee, Sara H.
Wang, Michael F. Z.
Heyang, Michael
Moral-Lopez, Pablo
Hinchman, Meleana M.
Danko, Charles G.
Parker, John S. L.
De Vlaminck, Iwijn
author_sort Saikia, Mridusmita
collection PubMed
description We describe Droplet Assisted RNA Targeting by single cell sequencing (DART-seq), a versatile technology that enables multiplexed amplicon sequencing and transcriptome profiling in single cells. We applied DART-seq to simultaneously characterize the non-A-tailed transcripts of a segmented dsRNA virus and the transcriptome of the infected cell. In addition, we used DART-seq to simultaneously determine the natively paired, variable region heavy and light chain amplicons and the transcriptome of B lymphocytes.
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spelling pubmed-63788782019-06-17 Simultaneous multiplexed amplicon sequencing and transcriptome profiling in single cells Saikia, Mridusmita Burnham, Philip Keshavjee, Sara H. Wang, Michael F. Z. Heyang, Michael Moral-Lopez, Pablo Hinchman, Meleana M. Danko, Charles G. Parker, John S. L. De Vlaminck, Iwijn Nat Methods Article We describe Droplet Assisted RNA Targeting by single cell sequencing (DART-seq), a versatile technology that enables multiplexed amplicon sequencing and transcriptome profiling in single cells. We applied DART-seq to simultaneously characterize the non-A-tailed transcripts of a segmented dsRNA virus and the transcriptome of the infected cell. In addition, we used DART-seq to simultaneously determine the natively paired, variable region heavy and light chain amplicons and the transcriptome of B lymphocytes. 2018-12-17 2019-01 /pmc/articles/PMC6378878/ /pubmed/30559431 http://dx.doi.org/10.1038/s41592-018-0259-9 Text en Users may view, print, copy, and download text and data-mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use:http://www.nature.com/authors/editorial_policies/license.html#terms
spellingShingle Article
Saikia, Mridusmita
Burnham, Philip
Keshavjee, Sara H.
Wang, Michael F. Z.
Heyang, Michael
Moral-Lopez, Pablo
Hinchman, Meleana M.
Danko, Charles G.
Parker, John S. L.
De Vlaminck, Iwijn
Simultaneous multiplexed amplicon sequencing and transcriptome profiling in single cells
title Simultaneous multiplexed amplicon sequencing and transcriptome profiling in single cells
title_full Simultaneous multiplexed amplicon sequencing and transcriptome profiling in single cells
title_fullStr Simultaneous multiplexed amplicon sequencing and transcriptome profiling in single cells
title_full_unstemmed Simultaneous multiplexed amplicon sequencing and transcriptome profiling in single cells
title_short Simultaneous multiplexed amplicon sequencing and transcriptome profiling in single cells
title_sort simultaneous multiplexed amplicon sequencing and transcriptome profiling in single cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6378878/
https://www.ncbi.nlm.nih.gov/pubmed/30559431
http://dx.doi.org/10.1038/s41592-018-0259-9
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