Cargando…

Chemoprotective effects of butanol fraction of Buchholzia coriacea (Capparidaceae) against type 2 diabetes and oxidative stress in male Wistar rats

Recent studies have shown that Type 2 diabetes (T2D) in rats can result through a synergy that links obesity to insulin resistance and β-cell dysfunction. The present study achieved T2D via high fructose (20%(w/v), p.o.), streptozotocin single dose (40 mg/kg, i.p.) (HFSTZ) in rats. Also, chemoprotec...

Descripción completa

Detalles Bibliográficos
Autores principales: Okolie, Amanda C., Kale, Oluwafemi E., Osilesi, Odutola
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Portland Press Ltd. 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6379225/
https://www.ncbi.nlm.nih.gov/pubmed/28790167
http://dx.doi.org/10.1042/BSR20170665
_version_ 1783396033736212480
author Okolie, Amanda C.
Kale, Oluwafemi E.
Osilesi, Odutola
author_facet Okolie, Amanda C.
Kale, Oluwafemi E.
Osilesi, Odutola
author_sort Okolie, Amanda C.
collection PubMed
description Recent studies have shown that Type 2 diabetes (T2D) in rats can result through a synergy that links obesity to insulin resistance and β-cell dysfunction. The present study achieved T2D via high fructose (20%(w/v), p.o.), streptozotocin single dose (40 mg/kg, i.p.) (HFSTZ) in rats. Also, chemoprotective potential of butanol fraction of Buchholzia coriacea (BFBC) was demonstrated. Control normal and diabetic untreated (HFSTZ-induced T2D) rats received CM-cellulose (1 mg/kg, p.o.). Diabetic rats received intragastric BFBC (20, 200, 400 mg/kg), glibenclamide (0.07 mg/kg), and BFBC (200 mg/kg) plus glibenclamide treatments, respectively. 2,2-Diphenyl-1-picrylhydrazyl, nitric oxide radical, hydroxyl radical scavenging activities, and α-amylase inhibition were assessed. After 2 weeks of treatments, blood glucose levels, lipid profiles, renal and liver function, serum insulin as well as in vivo oxidative stress biomarkers were assessed. BFBC shows highest antioxidants and α-amylase inhibitory activities in vitro. HFSTZ-induced T2D produced hyperglycemia (P<0.05–0.001; F = 5.26–26.47), serum hyperinsulinemia (six-folds) plus elevated lipid peroxidation levels. Similarly, there were altered lipid profiles, liver and renal biomarker enzymes plus weight loss. BFBC administration alone or in combination with glibenclamide reversed T2D symptomatologies in treated animals, and improved body weights against control diabetic rats. In vivo antioxidant activities also improved while histological sections in treated rats show reduced tissue damage in pancreas, kidneys, liver, and heart, respectively. Oleic, stearic, 2-methyl-pyrrolidine-2-carboxylic, and n-hexadecanoic acids were present in BFBC in large quantities given GC-MS analysis. Overall, data from the present study suggest chemoprotective potentials of BFBC against HFSTZ-induced T2D rats.
format Online
Article
Text
id pubmed-6379225
institution National Center for Biotechnology Information
language English
publishDate 2019
publisher Portland Press Ltd.
record_format MEDLINE/PubMed
spelling pubmed-63792252019-05-28 Chemoprotective effects of butanol fraction of Buchholzia coriacea (Capparidaceae) against type 2 diabetes and oxidative stress in male Wistar rats Okolie, Amanda C. Kale, Oluwafemi E. Osilesi, Odutola Biosci Rep Research Articles Recent studies have shown that Type 2 diabetes (T2D) in rats can result through a synergy that links obesity to insulin resistance and β-cell dysfunction. The present study achieved T2D via high fructose (20%(w/v), p.o.), streptozotocin single dose (40 mg/kg, i.p.) (HFSTZ) in rats. Also, chemoprotective potential of butanol fraction of Buchholzia coriacea (BFBC) was demonstrated. Control normal and diabetic untreated (HFSTZ-induced T2D) rats received CM-cellulose (1 mg/kg, p.o.). Diabetic rats received intragastric BFBC (20, 200, 400 mg/kg), glibenclamide (0.07 mg/kg), and BFBC (200 mg/kg) plus glibenclamide treatments, respectively. 2,2-Diphenyl-1-picrylhydrazyl, nitric oxide radical, hydroxyl radical scavenging activities, and α-amylase inhibition were assessed. After 2 weeks of treatments, blood glucose levels, lipid profiles, renal and liver function, serum insulin as well as in vivo oxidative stress biomarkers were assessed. BFBC shows highest antioxidants and α-amylase inhibitory activities in vitro. HFSTZ-induced T2D produced hyperglycemia (P<0.05–0.001; F = 5.26–26.47), serum hyperinsulinemia (six-folds) plus elevated lipid peroxidation levels. Similarly, there were altered lipid profiles, liver and renal biomarker enzymes plus weight loss. BFBC administration alone or in combination with glibenclamide reversed T2D symptomatologies in treated animals, and improved body weights against control diabetic rats. In vivo antioxidant activities also improved while histological sections in treated rats show reduced tissue damage in pancreas, kidneys, liver, and heart, respectively. Oleic, stearic, 2-methyl-pyrrolidine-2-carboxylic, and n-hexadecanoic acids were present in BFBC in large quantities given GC-MS analysis. Overall, data from the present study suggest chemoprotective potentials of BFBC against HFSTZ-induced T2D rats. Portland Press Ltd. 2019-02-15 /pmc/articles/PMC6379225/ /pubmed/28790167 http://dx.doi.org/10.1042/BSR20170665 Text en © 2019 The Author(s). http://creativecommons.org/licenses/by/4.0/This is an open access article published by Portland Press Limited on behalf of the Biochemical Society and distributed under the Creative Commons Attribution License 4.0 (CC BY) (http://creativecommons.org/licenses/by/4.0/) .
spellingShingle Research Articles
Okolie, Amanda C.
Kale, Oluwafemi E.
Osilesi, Odutola
Chemoprotective effects of butanol fraction of Buchholzia coriacea (Capparidaceae) against type 2 diabetes and oxidative stress in male Wistar rats
title Chemoprotective effects of butanol fraction of Buchholzia coriacea (Capparidaceae) against type 2 diabetes and oxidative stress in male Wistar rats
title_full Chemoprotective effects of butanol fraction of Buchholzia coriacea (Capparidaceae) against type 2 diabetes and oxidative stress in male Wistar rats
title_fullStr Chemoprotective effects of butanol fraction of Buchholzia coriacea (Capparidaceae) against type 2 diabetes and oxidative stress in male Wistar rats
title_full_unstemmed Chemoprotective effects of butanol fraction of Buchholzia coriacea (Capparidaceae) against type 2 diabetes and oxidative stress in male Wistar rats
title_short Chemoprotective effects of butanol fraction of Buchholzia coriacea (Capparidaceae) against type 2 diabetes and oxidative stress in male Wistar rats
title_sort chemoprotective effects of butanol fraction of buchholzia coriacea (capparidaceae) against type 2 diabetes and oxidative stress in male wistar rats
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6379225/
https://www.ncbi.nlm.nih.gov/pubmed/28790167
http://dx.doi.org/10.1042/BSR20170665
work_keys_str_mv AT okolieamandac chemoprotectiveeffectsofbutanolfractionofbuchholziacoriaceacapparidaceaeagainsttype2diabetesandoxidativestressinmalewistarrats
AT kaleoluwafemie chemoprotectiveeffectsofbutanolfractionofbuchholziacoriaceacapparidaceaeagainsttype2diabetesandoxidativestressinmalewistarrats
AT osilesiodutola chemoprotectiveeffectsofbutanolfractionofbuchholziacoriaceacapparidaceaeagainsttype2diabetesandoxidativestressinmalewistarrats