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Cell Type-Specific Transcriptomics of Lateral Root Formation and Plasticity

Lateral roots are a major determinant of root architecture and are instrumental for the efficient uptake of water and nutrients. Lateral roots consist of multiple cell types each expressing a unique transcriptome at a given developmental stage. Therefore, transcriptome analyses of complete lateral r...

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Detalles Bibliográficos
Autores principales: Kortz, Annika, Hochholdinger, Frank, Yu, Peng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6379339/
https://www.ncbi.nlm.nih.gov/pubmed/30809234
http://dx.doi.org/10.3389/fpls.2019.00021
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author Kortz, Annika
Hochholdinger, Frank
Yu, Peng
author_facet Kortz, Annika
Hochholdinger, Frank
Yu, Peng
author_sort Kortz, Annika
collection PubMed
description Lateral roots are a major determinant of root architecture and are instrumental for the efficient uptake of water and nutrients. Lateral roots consist of multiple cell types each expressing a unique transcriptome at a given developmental stage. Therefore, transcriptome analyses of complete lateral roots provide only average gene expression levels integrated over all cell types. Such analyses have the risk to mask genes, pathways and networks specifically expressed in a particular cell type during lateral root formation. Cell type-specific transcriptomics paves the way for a holistic understanding of the programming and re-programming of cells such as pericycle cells, involved in lateral root initiation. Recent discoveries have advanced the molecular understanding of the intrinsic genetic control of lateral root initiation and elongation. Moreover, the impact of nitrate availability on the transcriptional regulation of lateral root formation in Arabidopsis and cereals has been studied. In this review, we will focus on the systemic dissection of lateral root formation and its interaction with environmental nitrate through cell type-specific transcriptome analyses. These novel discoveries provide a better mechanistic understanding of postembryonic lateral root development in plants.
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spelling pubmed-63793392019-02-26 Cell Type-Specific Transcriptomics of Lateral Root Formation and Plasticity Kortz, Annika Hochholdinger, Frank Yu, Peng Front Plant Sci Plant Science Lateral roots are a major determinant of root architecture and are instrumental for the efficient uptake of water and nutrients. Lateral roots consist of multiple cell types each expressing a unique transcriptome at a given developmental stage. Therefore, transcriptome analyses of complete lateral roots provide only average gene expression levels integrated over all cell types. Such analyses have the risk to mask genes, pathways and networks specifically expressed in a particular cell type during lateral root formation. Cell type-specific transcriptomics paves the way for a holistic understanding of the programming and re-programming of cells such as pericycle cells, involved in lateral root initiation. Recent discoveries have advanced the molecular understanding of the intrinsic genetic control of lateral root initiation and elongation. Moreover, the impact of nitrate availability on the transcriptional regulation of lateral root formation in Arabidopsis and cereals has been studied. In this review, we will focus on the systemic dissection of lateral root formation and its interaction with environmental nitrate through cell type-specific transcriptome analyses. These novel discoveries provide a better mechanistic understanding of postembryonic lateral root development in plants. Frontiers Media S.A. 2019-02-12 /pmc/articles/PMC6379339/ /pubmed/30809234 http://dx.doi.org/10.3389/fpls.2019.00021 Text en Copyright © 2019 Kortz, Hochholdinger and Yu. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Kortz, Annika
Hochholdinger, Frank
Yu, Peng
Cell Type-Specific Transcriptomics of Lateral Root Formation and Plasticity
title Cell Type-Specific Transcriptomics of Lateral Root Formation and Plasticity
title_full Cell Type-Specific Transcriptomics of Lateral Root Formation and Plasticity
title_fullStr Cell Type-Specific Transcriptomics of Lateral Root Formation and Plasticity
title_full_unstemmed Cell Type-Specific Transcriptomics of Lateral Root Formation and Plasticity
title_short Cell Type-Specific Transcriptomics of Lateral Root Formation and Plasticity
title_sort cell type-specific transcriptomics of lateral root formation and plasticity
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6379339/
https://www.ncbi.nlm.nih.gov/pubmed/30809234
http://dx.doi.org/10.3389/fpls.2019.00021
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