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SAT: Free Software for the Semi-Automated Analysis of Rodent Brain Sections With 2,3,5-Triphenyltetrazolium Chloride Staining

Ischemic stroke places an increasing burden on individuals, families, and societies around the world. However, effective therapies or drugs for ischemic stroke are lacking. Therefore, animal models mimicking ischemic stroke in humans are of great value for preclinical experiments. middle cerebral ar...

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Autores principales: Shi, Xiao-Fang, Ai, Heng, Lu, Wen, Cai, Fuhong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6379447/
https://www.ncbi.nlm.nih.gov/pubmed/30809120
http://dx.doi.org/10.3389/fnins.2019.00102
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author Shi, Xiao-Fang
Ai, Heng
Lu, Wen
Cai, Fuhong
author_facet Shi, Xiao-Fang
Ai, Heng
Lu, Wen
Cai, Fuhong
author_sort Shi, Xiao-Fang
collection PubMed
description Ischemic stroke places an increasing burden on individuals, families, and societies around the world. However, effective therapies or drugs for ischemic stroke are lacking. Therefore, animal models mimicking ischemic stroke in humans are of great value for preclinical experiments. middle cerebral artery occlusion (MCAO) in mice or rats and subsequent 2,3,5-triphenyltetrazolium chloride (TTC) staining of brain sections are common methods in the study of experimental animal ischemic stroke. In this study, we present and assess the utility of the semi-automated analysis of the TTC staining (SAT) software program, a novel, small, user-friendly, and free software program, in the quantification of the infarct size in rodent brain sections, with TTC staining, by analyzing images captured by cell phones or scan systems. We performed MCAO and TTC staining in adult mice. We then utilized the SAT software and Image J to analyze the infarct size in the brain sections with TTC staining and compared the findings of the two analysis methods. We found that the data on infarct size from SAT and from Image J were comparable, suggesting that the SAT software could be an alternative option to Image J in the evaluation of ischemic stroke.
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spelling pubmed-63794472019-02-26 SAT: Free Software for the Semi-Automated Analysis of Rodent Brain Sections With 2,3,5-Triphenyltetrazolium Chloride Staining Shi, Xiao-Fang Ai, Heng Lu, Wen Cai, Fuhong Front Neurosci Neuroscience Ischemic stroke places an increasing burden on individuals, families, and societies around the world. However, effective therapies or drugs for ischemic stroke are lacking. Therefore, animal models mimicking ischemic stroke in humans are of great value for preclinical experiments. middle cerebral artery occlusion (MCAO) in mice or rats and subsequent 2,3,5-triphenyltetrazolium chloride (TTC) staining of brain sections are common methods in the study of experimental animal ischemic stroke. In this study, we present and assess the utility of the semi-automated analysis of the TTC staining (SAT) software program, a novel, small, user-friendly, and free software program, in the quantification of the infarct size in rodent brain sections, with TTC staining, by analyzing images captured by cell phones or scan systems. We performed MCAO and TTC staining in adult mice. We then utilized the SAT software and Image J to analyze the infarct size in the brain sections with TTC staining and compared the findings of the two analysis methods. We found that the data on infarct size from SAT and from Image J were comparable, suggesting that the SAT software could be an alternative option to Image J in the evaluation of ischemic stroke. Frontiers Media S.A. 2019-02-12 /pmc/articles/PMC6379447/ /pubmed/30809120 http://dx.doi.org/10.3389/fnins.2019.00102 Text en Copyright © 2019 Shi, Ai, Lu and Cai. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Neuroscience
Shi, Xiao-Fang
Ai, Heng
Lu, Wen
Cai, Fuhong
SAT: Free Software for the Semi-Automated Analysis of Rodent Brain Sections With 2,3,5-Triphenyltetrazolium Chloride Staining
title SAT: Free Software for the Semi-Automated Analysis of Rodent Brain Sections With 2,3,5-Triphenyltetrazolium Chloride Staining
title_full SAT: Free Software for the Semi-Automated Analysis of Rodent Brain Sections With 2,3,5-Triphenyltetrazolium Chloride Staining
title_fullStr SAT: Free Software for the Semi-Automated Analysis of Rodent Brain Sections With 2,3,5-Triphenyltetrazolium Chloride Staining
title_full_unstemmed SAT: Free Software for the Semi-Automated Analysis of Rodent Brain Sections With 2,3,5-Triphenyltetrazolium Chloride Staining
title_short SAT: Free Software for the Semi-Automated Analysis of Rodent Brain Sections With 2,3,5-Triphenyltetrazolium Chloride Staining
title_sort sat: free software for the semi-automated analysis of rodent brain sections with 2,3,5-triphenyltetrazolium chloride staining
topic Neuroscience
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6379447/
https://www.ncbi.nlm.nih.gov/pubmed/30809120
http://dx.doi.org/10.3389/fnins.2019.00102
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