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Biomethane production from sugar beet pulp under cocultivation with Clostridium cellulovorans and methanogens
This study was demonstrated with a coculture fermentation system using sugar beet pulp (SBP) as a carbon source combining the cellulose-degrading bacterium Clostridium cellulovorans with microbial flora of methane production (MFMP) for the direct conversion of cellulosic biomass to methane (CH(4))....
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Springer Berlin Heidelberg
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6379495/ https://www.ncbi.nlm.nih.gov/pubmed/30778890 http://dx.doi.org/10.1186/s13568-019-0752-2 |
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author | Tomita, Hisao Okazaki, Fumiyoshi Tamaru, Yutaka |
author_facet | Tomita, Hisao Okazaki, Fumiyoshi Tamaru, Yutaka |
author_sort | Tomita, Hisao |
collection | PubMed |
description | This study was demonstrated with a coculture fermentation system using sugar beet pulp (SBP) as a carbon source combining the cellulose-degrading bacterium Clostridium cellulovorans with microbial flora of methane production (MFMP) for the direct conversion of cellulosic biomass to methane (CH(4)). The MFMP was taken from a commercial methane fermentation plant and extremely complicated. Therefore, the MFMP was analyzed by a next-generation sequencing system and the microbiome was identified and classified based on several computer programs. As a result, Methanosarcina mazei (1.34% of total counts) and the other methanogens were found in the MFMP. Interestingly, the simultaneous utilization of hydrogen (H(2)) and carbon dioxide (CO(2)) for methanogenesis was observed in the coculture with Consortium of C. cellulovorans with the MFMP (CCeM) including M. mazei. Furthermore, the CCeM degraded 87.3% of SBP without any pretreatment and produced 34.0 L of CH(4) per 1 kg of dry weight of SBP. Thus, a gas metabolic shift in the fermentation pattern of C. cellulovorans was observed in the CCeM coculture. These results indicated that degradation of agricultural wastes was able to be carried out simultaneously with CH(4) production by C. cellulovorans and the MFMP. |
format | Online Article Text |
id | pubmed-6379495 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Springer Berlin Heidelberg |
record_format | MEDLINE/PubMed |
spelling | pubmed-63794952019-03-10 Biomethane production from sugar beet pulp under cocultivation with Clostridium cellulovorans and methanogens Tomita, Hisao Okazaki, Fumiyoshi Tamaru, Yutaka AMB Express Original Article This study was demonstrated with a coculture fermentation system using sugar beet pulp (SBP) as a carbon source combining the cellulose-degrading bacterium Clostridium cellulovorans with microbial flora of methane production (MFMP) for the direct conversion of cellulosic biomass to methane (CH(4)). The MFMP was taken from a commercial methane fermentation plant and extremely complicated. Therefore, the MFMP was analyzed by a next-generation sequencing system and the microbiome was identified and classified based on several computer programs. As a result, Methanosarcina mazei (1.34% of total counts) and the other methanogens were found in the MFMP. Interestingly, the simultaneous utilization of hydrogen (H(2)) and carbon dioxide (CO(2)) for methanogenesis was observed in the coculture with Consortium of C. cellulovorans with the MFMP (CCeM) including M. mazei. Furthermore, the CCeM degraded 87.3% of SBP without any pretreatment and produced 34.0 L of CH(4) per 1 kg of dry weight of SBP. Thus, a gas metabolic shift in the fermentation pattern of C. cellulovorans was observed in the CCeM coculture. These results indicated that degradation of agricultural wastes was able to be carried out simultaneously with CH(4) production by C. cellulovorans and the MFMP. Springer Berlin Heidelberg 2019-02-18 /pmc/articles/PMC6379495/ /pubmed/30778890 http://dx.doi.org/10.1186/s13568-019-0752-2 Text en © The Author(s) 2019 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. |
spellingShingle | Original Article Tomita, Hisao Okazaki, Fumiyoshi Tamaru, Yutaka Biomethane production from sugar beet pulp under cocultivation with Clostridium cellulovorans and methanogens |
title | Biomethane production from sugar beet pulp under cocultivation with Clostridium cellulovorans and methanogens |
title_full | Biomethane production from sugar beet pulp under cocultivation with Clostridium cellulovorans and methanogens |
title_fullStr | Biomethane production from sugar beet pulp under cocultivation with Clostridium cellulovorans and methanogens |
title_full_unstemmed | Biomethane production from sugar beet pulp under cocultivation with Clostridium cellulovorans and methanogens |
title_short | Biomethane production from sugar beet pulp under cocultivation with Clostridium cellulovorans and methanogens |
title_sort | biomethane production from sugar beet pulp under cocultivation with clostridium cellulovorans and methanogens |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6379495/ https://www.ncbi.nlm.nih.gov/pubmed/30778890 http://dx.doi.org/10.1186/s13568-019-0752-2 |
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