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Ascorbic acid stimulates the in vitro myoblast proliferation and migration of pacu (Piaractus mesopotamicus)

The postembryonic growth of skeletal muscle in teleost fish involves myoblast proliferation, migration and differentiation, encompassing the main events of embryonic myogenesis. Ascorbic acid plays important cellular and biochemical roles as an antioxidant and contributes to the proper collagen bios...

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Autores principales: Duran, Bruno Oliveira Silva, Góes, Guilherme Alcarás, Zanella, Bruna Tereza Thomazini, Freire, Paula Paccielli, Valente, Jessica Silvino, Salomão, Rondinelle Artur Simões, Fernandes, Ana, Mareco, Edson Assunção, Carvalho, Robson Francisco, Dal-Pai-Silva, Maeli
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6379551/
https://www.ncbi.nlm.nih.gov/pubmed/30778153
http://dx.doi.org/10.1038/s41598-019-38536-4
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author Duran, Bruno Oliveira Silva
Góes, Guilherme Alcarás
Zanella, Bruna Tereza Thomazini
Freire, Paula Paccielli
Valente, Jessica Silvino
Salomão, Rondinelle Artur Simões
Fernandes, Ana
Mareco, Edson Assunção
Carvalho, Robson Francisco
Dal-Pai-Silva, Maeli
author_facet Duran, Bruno Oliveira Silva
Góes, Guilherme Alcarás
Zanella, Bruna Tereza Thomazini
Freire, Paula Paccielli
Valente, Jessica Silvino
Salomão, Rondinelle Artur Simões
Fernandes, Ana
Mareco, Edson Assunção
Carvalho, Robson Francisco
Dal-Pai-Silva, Maeli
author_sort Duran, Bruno Oliveira Silva
collection PubMed
description The postembryonic growth of skeletal muscle in teleost fish involves myoblast proliferation, migration and differentiation, encompassing the main events of embryonic myogenesis. Ascorbic acid plays important cellular and biochemical roles as an antioxidant and contributes to the proper collagen biosynthesis necessary for the structure of connective and bone tissues. However, whether ascorbic acid can directly influence the mechanisms of fish myogenesis and skeletal muscle growth remains unclear. The aim of our work was to evaluate the effects of ascorbic acid supplementation on the in vitro myoblast proliferation and migration of pacu (Piaractus mesopotamicus). To provide insight into the potential antioxidant role of ascorbic acid, we also treated myoblasts in vitro with menadione, which is a powerful oxidant. Our results show that ascorbic acid-supplemented myoblasts exhibit increased proliferation and migration and are protected against the oxidative stress caused by menadione. In addition, ascorbic acid increased the activity of the antioxidant enzyme superoxide dismutase and the expression of myog and mtor, which are molecular markers related to skeletal muscle myogenesis and protein synthesis, respectively. This work reveals a direct influence of ascorbic acid on the mechanisms of pacu myogenesis and highlights the potential use of ascorbic acid for stimulating fish skeletal muscle growth.
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spelling pubmed-63795512019-02-21 Ascorbic acid stimulates the in vitro myoblast proliferation and migration of pacu (Piaractus mesopotamicus) Duran, Bruno Oliveira Silva Góes, Guilherme Alcarás Zanella, Bruna Tereza Thomazini Freire, Paula Paccielli Valente, Jessica Silvino Salomão, Rondinelle Artur Simões Fernandes, Ana Mareco, Edson Assunção Carvalho, Robson Francisco Dal-Pai-Silva, Maeli Sci Rep Article The postembryonic growth of skeletal muscle in teleost fish involves myoblast proliferation, migration and differentiation, encompassing the main events of embryonic myogenesis. Ascorbic acid plays important cellular and biochemical roles as an antioxidant and contributes to the proper collagen biosynthesis necessary for the structure of connective and bone tissues. However, whether ascorbic acid can directly influence the mechanisms of fish myogenesis and skeletal muscle growth remains unclear. The aim of our work was to evaluate the effects of ascorbic acid supplementation on the in vitro myoblast proliferation and migration of pacu (Piaractus mesopotamicus). To provide insight into the potential antioxidant role of ascorbic acid, we also treated myoblasts in vitro with menadione, which is a powerful oxidant. Our results show that ascorbic acid-supplemented myoblasts exhibit increased proliferation and migration and are protected against the oxidative stress caused by menadione. In addition, ascorbic acid increased the activity of the antioxidant enzyme superoxide dismutase and the expression of myog and mtor, which are molecular markers related to skeletal muscle myogenesis and protein synthesis, respectively. This work reveals a direct influence of ascorbic acid on the mechanisms of pacu myogenesis and highlights the potential use of ascorbic acid for stimulating fish skeletal muscle growth. Nature Publishing Group UK 2019-02-18 /pmc/articles/PMC6379551/ /pubmed/30778153 http://dx.doi.org/10.1038/s41598-019-38536-4 Text en © The Author(s) 2019 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Duran, Bruno Oliveira Silva
Góes, Guilherme Alcarás
Zanella, Bruna Tereza Thomazini
Freire, Paula Paccielli
Valente, Jessica Silvino
Salomão, Rondinelle Artur Simões
Fernandes, Ana
Mareco, Edson Assunção
Carvalho, Robson Francisco
Dal-Pai-Silva, Maeli
Ascorbic acid stimulates the in vitro myoblast proliferation and migration of pacu (Piaractus mesopotamicus)
title Ascorbic acid stimulates the in vitro myoblast proliferation and migration of pacu (Piaractus mesopotamicus)
title_full Ascorbic acid stimulates the in vitro myoblast proliferation and migration of pacu (Piaractus mesopotamicus)
title_fullStr Ascorbic acid stimulates the in vitro myoblast proliferation and migration of pacu (Piaractus mesopotamicus)
title_full_unstemmed Ascorbic acid stimulates the in vitro myoblast proliferation and migration of pacu (Piaractus mesopotamicus)
title_short Ascorbic acid stimulates the in vitro myoblast proliferation and migration of pacu (Piaractus mesopotamicus)
title_sort ascorbic acid stimulates the in vitro myoblast proliferation and migration of pacu (piaractus mesopotamicus)
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6379551/
https://www.ncbi.nlm.nih.gov/pubmed/30778153
http://dx.doi.org/10.1038/s41598-019-38536-4
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