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Ascorbic acid stimulates the in vitro myoblast proliferation and migration of pacu (Piaractus mesopotamicus)
The postembryonic growth of skeletal muscle in teleost fish involves myoblast proliferation, migration and differentiation, encompassing the main events of embryonic myogenesis. Ascorbic acid plays important cellular and biochemical roles as an antioxidant and contributes to the proper collagen bios...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6379551/ https://www.ncbi.nlm.nih.gov/pubmed/30778153 http://dx.doi.org/10.1038/s41598-019-38536-4 |
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author | Duran, Bruno Oliveira Silva Góes, Guilherme Alcarás Zanella, Bruna Tereza Thomazini Freire, Paula Paccielli Valente, Jessica Silvino Salomão, Rondinelle Artur Simões Fernandes, Ana Mareco, Edson Assunção Carvalho, Robson Francisco Dal-Pai-Silva, Maeli |
author_facet | Duran, Bruno Oliveira Silva Góes, Guilherme Alcarás Zanella, Bruna Tereza Thomazini Freire, Paula Paccielli Valente, Jessica Silvino Salomão, Rondinelle Artur Simões Fernandes, Ana Mareco, Edson Assunção Carvalho, Robson Francisco Dal-Pai-Silva, Maeli |
author_sort | Duran, Bruno Oliveira Silva |
collection | PubMed |
description | The postembryonic growth of skeletal muscle in teleost fish involves myoblast proliferation, migration and differentiation, encompassing the main events of embryonic myogenesis. Ascorbic acid plays important cellular and biochemical roles as an antioxidant and contributes to the proper collagen biosynthesis necessary for the structure of connective and bone tissues. However, whether ascorbic acid can directly influence the mechanisms of fish myogenesis and skeletal muscle growth remains unclear. The aim of our work was to evaluate the effects of ascorbic acid supplementation on the in vitro myoblast proliferation and migration of pacu (Piaractus mesopotamicus). To provide insight into the potential antioxidant role of ascorbic acid, we also treated myoblasts in vitro with menadione, which is a powerful oxidant. Our results show that ascorbic acid-supplemented myoblasts exhibit increased proliferation and migration and are protected against the oxidative stress caused by menadione. In addition, ascorbic acid increased the activity of the antioxidant enzyme superoxide dismutase and the expression of myog and mtor, which are molecular markers related to skeletal muscle myogenesis and protein synthesis, respectively. This work reveals a direct influence of ascorbic acid on the mechanisms of pacu myogenesis and highlights the potential use of ascorbic acid for stimulating fish skeletal muscle growth. |
format | Online Article Text |
id | pubmed-6379551 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-63795512019-02-21 Ascorbic acid stimulates the in vitro myoblast proliferation and migration of pacu (Piaractus mesopotamicus) Duran, Bruno Oliveira Silva Góes, Guilherme Alcarás Zanella, Bruna Tereza Thomazini Freire, Paula Paccielli Valente, Jessica Silvino Salomão, Rondinelle Artur Simões Fernandes, Ana Mareco, Edson Assunção Carvalho, Robson Francisco Dal-Pai-Silva, Maeli Sci Rep Article The postembryonic growth of skeletal muscle in teleost fish involves myoblast proliferation, migration and differentiation, encompassing the main events of embryonic myogenesis. Ascorbic acid plays important cellular and biochemical roles as an antioxidant and contributes to the proper collagen biosynthesis necessary for the structure of connective and bone tissues. However, whether ascorbic acid can directly influence the mechanisms of fish myogenesis and skeletal muscle growth remains unclear. The aim of our work was to evaluate the effects of ascorbic acid supplementation on the in vitro myoblast proliferation and migration of pacu (Piaractus mesopotamicus). To provide insight into the potential antioxidant role of ascorbic acid, we also treated myoblasts in vitro with menadione, which is a powerful oxidant. Our results show that ascorbic acid-supplemented myoblasts exhibit increased proliferation and migration and are protected against the oxidative stress caused by menadione. In addition, ascorbic acid increased the activity of the antioxidant enzyme superoxide dismutase and the expression of myog and mtor, which are molecular markers related to skeletal muscle myogenesis and protein synthesis, respectively. This work reveals a direct influence of ascorbic acid on the mechanisms of pacu myogenesis and highlights the potential use of ascorbic acid for stimulating fish skeletal muscle growth. Nature Publishing Group UK 2019-02-18 /pmc/articles/PMC6379551/ /pubmed/30778153 http://dx.doi.org/10.1038/s41598-019-38536-4 Text en © The Author(s) 2019 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Duran, Bruno Oliveira Silva Góes, Guilherme Alcarás Zanella, Bruna Tereza Thomazini Freire, Paula Paccielli Valente, Jessica Silvino Salomão, Rondinelle Artur Simões Fernandes, Ana Mareco, Edson Assunção Carvalho, Robson Francisco Dal-Pai-Silva, Maeli Ascorbic acid stimulates the in vitro myoblast proliferation and migration of pacu (Piaractus mesopotamicus) |
title | Ascorbic acid stimulates the in vitro myoblast proliferation and migration of pacu (Piaractus mesopotamicus) |
title_full | Ascorbic acid stimulates the in vitro myoblast proliferation and migration of pacu (Piaractus mesopotamicus) |
title_fullStr | Ascorbic acid stimulates the in vitro myoblast proliferation and migration of pacu (Piaractus mesopotamicus) |
title_full_unstemmed | Ascorbic acid stimulates the in vitro myoblast proliferation and migration of pacu (Piaractus mesopotamicus) |
title_short | Ascorbic acid stimulates the in vitro myoblast proliferation and migration of pacu (Piaractus mesopotamicus) |
title_sort | ascorbic acid stimulates the in vitro myoblast proliferation and migration of pacu (piaractus mesopotamicus) |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6379551/ https://www.ncbi.nlm.nih.gov/pubmed/30778153 http://dx.doi.org/10.1038/s41598-019-38536-4 |
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