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Exonuclease III (XthA) Enforces In Vivo DNA Cloning of Escherichia coli To Create Cohesive Ends

Escherichia coli has an ability to assemble DNA fragments with homologous overlapping sequences of 15 to 40 bp at each end. Several modified protocols have already been reported to improve this simple and useful DNA cloning technology. However, the molecular mechanism by which E. coli accomplishes s...

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Detalles Bibliográficos
Autores principales: Nozaki, Shingo, Niki, Hironori
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Society for Microbiology 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6379578/
https://www.ncbi.nlm.nih.gov/pubmed/30530516
http://dx.doi.org/10.1128/JB.00660-18

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